BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_K2139010 1 BBa_K2139010 Gluc1C with Terminator 2016-10-13T11:00:00Z 2016-10-30T04:00:06Z Codon optimized for Caulobacter crescentus (high GC content. Gluc1C is a monomeric enzyme capable of catalyzing the conversion of cellobiose into monomeric glucose at the non reducing end of the sugar polymer. It can be used in conjunction with an endoglucanase for the direct conversion of cellulose into monomeric glucose. The genbank ascension number for this protein is JQ713769.1 (DNA), AFQ36783.1 (amino acid), and a PDB code of 2O9R_A. This construct was synthesized for use in Caulobacter cresentus and is codon optimized to contain a high GC content. Literature data for this part can be found http://www.sciencedirect.com/science/article/pii/S1046592812003154 false false _2609_ 33491 34108 9 false Paenibacillus sp. MTCC 5639 false UBC iGEM 2016 component2500999 1 BBa_K2139002 component2501006 1 BBa_B0015 annotation2501006 1 BBa_B0015 range2501006 1 1356 1484 annotation2500999 1 BBa_K2139002 range2500999 1 1 1347 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916612 1 BBa_B0012 component1916610 1 BBa_B0010 annotation1916610 1 BBa_B0010 range1916610 1 1 80 annotation1916612 1 BBa_B0012 range1916612 1 89 129 BBa_K2139002 1 BBa_K2139002 Coding Sequence of Gluc1C 2016-10-12T11:00:00Z 2016-10-30T03:51:13Z Paenibacillus sp. MTCC 5639 BBa_K2139000 encompass the coding region for an 1,4-beta-glucosidase gene known as Gluc1C. Gluc1C is a monomeric enyme capable of catalyzing the conversion of cellobiose into monomeric glucose at the non reducing end of the sugar polymer. It can be used in conjunction with an endoglucanase for the direct conversion of cellulose into monomeric glucose. The genbank ascension number for this protein is JQ713769.1 (DNA), AFQ36783.1 (amino acid), and a PDB code of 2O9R_A. This construct was synthesized for use in Caulobacter cresentus and is codon optimized to contain a high GC content. Literature data for this part can be found http://www.sciencedirect.com/science/article/pii/S1046592812003154 false false _2609_ 33491 34108 9 false Codon optimized for Caulobacter cresentus (high GC content) false UBC iGEM 2016 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K2139010_sequence 1 atgagcgagaacacgttcatctttccggcgaccttcatgtgggggacgtcgacgtcctcgtaccagatcgagggcgggacggacgaaggcgggcgcacccccagcatctgggatacgttttgtcaaatccccggcaaggtcatcgggggcgattgcggcgatgtcgcctgcgatcacttccaccactttaaggaggacgtgcagctgatgaagcagctcggctttctgcattaccgtttcagcgtggcctggccgcgcatcatgccggccccgggcatcatcaacgaggaaggcctcctgttctatgagcatctgctcgacgaaatcgaactcgccgggctcatccccatgctcaccctctatcactgggatctcccgcagtggatcgaggatgagggggggtggacgcagcgtgagacgatccagcacttcaagacctacgcgagcgtgatcatggaccgtttcggggaacgcatcaactggtggaacacgatcaacgaaccgtattgcgcctccatcctcgggtatgggacgggggaacatgcccccggccacgagaactggcgggaggccttcaccgccgcccatcacatcctgatgtgtcacggcatcgcctcgaacctgcataaggaaaaggggctgaccgggaagatcgggatcaccctcaatatggagcacgtggacgccgccagcgagcgccccgaagacgtcgcggcggcgatccgccgtgatggcttcatcaaccgttggtttgcggaacccctctttaacgggaagtacccggaagatatggtcgagtggtacggcacctacctcaacgggctggactttgtgcagcccggcgatatggaactgatccaacaacccggcgattttctgggcatcaattactacacgcgtagcatcatccggtcgaccaatgacgcgtccctcctccaggtggagcaagtccacatggaggaacccgtcaccgacatgggctgggagatccatcccgagtcgttctataagctcctcacccgcatcgagaaggacttcagcaagggcctccccatcctcatcaccgaaaacggggccgcgatgcgcgatgaggtggtgaacggccagatcgaagacaccggccgccagcgttatatcgaagagcatctgaaggcgtgtcaccgtttcatcgaagagggggggcaactcaaggggtatttcgtctggtcgtttctcgacaactttgagtgggcgtgggggtacagcaagcgcttcgggatcgtgcatatcaattatgaaacgcaagagcggaccccgaagcaatccgccctctggtttaagcaaatgatggccaagaacgggttctaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K2139002_sequence 1 atgagcgagaacacgttcatctttccggcgaccttcatgtgggggacgtcgacgtcctcgtaccagatcgagggcgggacggacgaaggcgggcgcacccccagcatctgggatacgttttgtcaaatccccggcaaggtcatcgggggcgattgcggcgatgtcgcctgcgatcacttccaccactttaaggaggacgtgcagctgatgaagcagctcggctttctgcattaccgtttcagcgtggcctggccgcgcatcatgccggccccgggcatcatcaacgaggaaggcctcctgttctatgagcatctgctcgacgaaatcgaactcgccgggctcatccccatgctcaccctctatcactgggatctcccgcagtggatcgaggatgagggggggtggacgcagcgtgagacgatccagcacttcaagacctacgcgagcgtgatcatggaccgtttcggggaacgcatcaactggtggaacacgatcaacgaaccgtattgcgcctccatcctcgggtatgggacgggggaacatgcccccggccacgagaactggcgggaggccttcaccgccgcccatcacatcctgatgtgtcacggcatcgcctcgaacctgcataaggaaaaggggctgaccgggaagatcgggatcaccctcaatatggagcacgtggacgccgccagcgagcgccccgaagacgtcgcggcggcgatccgccgtgatggcttcatcaaccgttggtttgcggaacccctctttaacgggaagtacccggaagatatggtcgagtggtacggcacctacctcaacgggctggactttgtgcagcccggcgatatggaactgatccaacaacccggcgattttctgggcatcaattactacacgcgtagcatcatccggtcgaccaatgacgcgtccctcctccaggtggagcaagtccacatggaggaacccgtcaccgacatgggctgggagatccatcccgagtcgttctataagctcctcacccgcatcgagaaggacttcagcaagggcctccccatcctcatcaccgaaaacggggccgcgatgcgcgatgaggtggtgaacggccagatcgaagacaccggccgccagcgttatatcgaagagcatctgaaggcgtgtcaccgtttcatcgaagagggggggcaactcaaggggtatttcgtctggtcgtttctcgacaactttgagtgggcgtgggggtacagcaagcgcttcgggatcgtgcatatcaattatgaaacgcaagagcggaccccgaagcaatccgccctctggtttaagcaaatgatggccaagaacgggttctaa BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z