BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_K2139001 1 BBa_K2139001 Coding Sequence of Endo5a 2016-10-12T11:00:00Z 2016-10-30T03:50:32Z Paenibacillus strain ICGEB2008 BBa_K2139000 encompass the coding region for a endo-beta-1,4-glucanase known as Endo5A. Endo5a catalyzes the conversion of cellulose into smaller fragments by making internal cuts in the cellulose chain. It can be used in conjunction with exoglucanases for the direct conversion of cellulose into monomeric glucose. The polymerization of the protein is monomeric with an optimal temperature of 50 degrees Celsius and pH ranging from 6 to 8. The genbank ascension number for this protein is HQ657203.1 (DNA), AEB00655.1 (amino acid) ,and a PDB code of 1VRX. This construct was synthesized for use in Caulobacter Cresentus and as such as codon optimized to contain a high GC content. Literature data for this part can be found http://www.sciencedirect.com/science/article/pii/S1046592812003154 false false _2609_ 33491 34108 9 false It is codon optimized for Caulobacter cresentus, exhibiting a high GC content. false UBC iGEM 2016 BBa_K2139015 1 BBa_K2139015 Endo5a with terminator 2016-10-13T11:00:00Z 2016-10-30T04:04:02Z Codon optimized for E. coli. E1 catalyzes the conversion of cellulose into smaller fragments by making internal cuts in the cellulose chain. It can be used in conjunction with exoglucanases for the direct conversion of cellulose into monomeric glucose. The polymerization of the protein is monomeric with an optimal temperature and pH over a wide range (unpublished data). The genbank ascension number for this protein is WP_011719450.1 (amino acid) and a PDB code of 1ECE. This construct was codon optimized for E. coli. Literature data for this part can be found http://aem.asm.org/content/76/19/6360.full false false _2609_ 33491 34108 9 false Acidothermus cellulolyticus false UBC iGEM 2016 component2527445 1 BBa_K2139001 component2527452 1 BBa_B0015 annotation2527452 1 BBa_B0015 range2527452 1 1203 1331 annotation2527445 1 BBa_K2139001 range2527445 1 1 1194 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916610 1 BBa_B0010 component1916612 1 BBa_B0012 annotation1916610 1 BBa_B0010 range1916610 1 1 80 annotation1916612 1 BBa_B0012 range1916612 1 89 129 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 annotation1687 1 stop range1687 1 34 34 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K2139015_sequence 1 atgaagaagaagggcctgaagaagaccttctttgtcatcgcgagcctcgtcatggggctgaccctctacggctataccccgccgtccgcggacgccgccagcgtcaaggggtattaccacacccagggcaacaagatcgtcgatgagagcggcaaggaggcggccttcaatgggctcaattggttcggcctggaaacccccaattataccctgcatgggctgtggtcgcggtcgatggatgacatgctcgaccaagtcaagaaggaggggtacaatctcatccggctgccctacagcaatcagctcttcgattcgagctcgcgcgcggattccatcgattactataagaacccggatctggtcggcctgacgccgatccagatcatggacaagctgatcgagaaggccggccagcgtggcatccaaatcatcctggatcgccatcgccccgggtccgggggccagtcggagctgtggtatacctcgcagtacccggaatcccggtggatctccgattggaagatgctcgccgaacgttacaagaataacccgaccgtgatcggggcggacctgcacaacgagccccacggccaagccagctgggggaccggggatgtgagcaccgactggcgtctggccgcgcaacgtgcgggcaacgcgatcctctccgtgaacccgaattggctgatcctggtggaaggcgtcgaccacaatgtccagggcaacaattcccagtattggtggggcggcaacctgaccggcgtggccaactaccccgtcgtcctggatgtccccaaccgggtcgtctattccccgcatgattacgggccgggggtgtcgagccaaccctggttcaacgactcgacgtttccgagcaatctccccgccatctgggaccaaacctggggctatatctccaagcaaaacatcgcgccggtgctcgtgggcgagtttggcgggcgcaacgtggatagctcgagcccggaaggcaagtggcaaaatgcgctcgtggactacatcggggcgaacaacctctattttacctactggtccctcaaccccaattcgggcgataccggcggcctgctcctcgacgattgggtgacgtggaatcgcccgaagcaagacatgctgagccggatcatgaagccggtgaactttatcgcggaacaagcgaaggcctccgcggagtagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K2139001_sequence 1 atgaagaagaagggcctgaagaagaccttctttgtcatcgcgagcctcgtcatggggctgaccctctacggctataccccgccgtccgcggacgccgccagcgtcaaggggtattaccacacccagggcaacaagatcgtcgatgagagcggcaaggaggcggccttcaatgggctcaattggttcggcctggaaacccccaattataccctgcatgggctgtggtcgcggtcgatggatgacatgctcgaccaagtcaagaaggaggggtacaatctcatccggctgccctacagcaatcagctcttcgattcgagctcgcgcgcggattccatcgattactataagaacccggatctggtcggcctgacgccgatccagatcatggacaagctgatcgagaaggccggccagcgtggcatccaaatcatcctggatcgccatcgccccgggtccgggggccagtcggagctgtggtatacctcgcagtacccggaatcccggtggatctccgattggaagatgctcgccgaacgttacaagaataacccgaccgtgatcggggcggacctgcacaacgagccccacggccaagccagctgggggaccggggatgtgagcaccgactggcgtctggccgcgcaacgtgcgggcaacgcgatcctctccgtgaacccgaattggctgatcctggtggaaggcgtcgaccacaatgtccagggcaacaattcccagtattggtggggcggcaacctgaccggcgtggccaactaccccgtcgtcctggatgtccccaaccgggtcgtctattccccgcatgattacgggccgggggtgtcgagccaaccctggttcaacgactcgacgtttccgagcaatctccccgccatctgggaccaaacctggggctatatctccaagcaaaacatcgcgccggtgctcgtgggcgagtttggcgggcgcaacgtggatagctcgagcccggaaggcaagtggcaaaatgcgctcgtggactacatcggggcgaacaacctctattttacctactggtccctcaaccccaattcgggcgataccggcggcctgctcctcgacgattgggtgacgtggaatcgcccgaagcaagacatgctgagccggatcatgaagccggtgaactttatcgcggaacaagcgaaggcctccgcggagtag BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z