BBa_K2140005 1 BBa_K2140005 StarScafold NonSequential 1 2016-10-12T11:00:00Z 2016-10-13T04:36:23Z The Leucine Zipper, Linker and Cystine regions we're designed denovo from the literature, while The inteins are a combination of engineered and non-engineered genomic gene segments. 1. Stevens, A. J., Brown, Z. Z., Shah, N. H., Sekar, G., Cowburn, D., & Muir, T. W. (2016). Design of a Split Intein with Exceptional Protein Splicing Activity. Journal of the American Chemical Society, 138(7), 2162-2165. 2. Carvajal-Vallejos, P., Palliss??, R., Mootz, H. D., & Schmidt, S. R. (2012). Unprecedented rates and efficiencies revealed for new natural split inteins from metagenomic sources. Journal of Biological Chemistry, 287(34), 28686-28696. 3. Dassa, B., London, N., Stoddard, B. L., Schueler-Furman, O., & Pietrokovski, S. (2009). Fractured genes: a novel genomic arrangement involving new split inteins and a new homing endonuclease family. Nucleic acids research, gkp095. Sequential intein activation is not required for enzyme splicing, we have a set of four previously tested inteins we can use for enzyme co-localisation which have been shown to have a high splicing rate, yield and orthologous splicing reactions. This part contains the coding sequence for our Non-Sequential StarScaffold, which allows us to check structural changes that occur due to splicing reactions occurring in the same mixture but at alternate temperatures. false false _2610_ 31465 31465 9 false The length of the linker and leucine zipper regions along with the order of the intein segments. false Robert Ashley Naturani annotation2497201 1 ATG (reverse compliment) range2497201 1 1306 1308 BBa_K2140005_sequence 1 cccttgaaagtaaagattctccgaacccccacctcctttcgcacgagaacgcaagaaatctccgacttttaaaccgctattaatcgtttttaagccgtctttagtgggaaatttgtgattagcagagacgcggatttttttcccagatttactcttcaggacaatctcgaaaaccggttgtttgataatcggtaacacttccgtcacctgtacaggtccacactcgctttcaagccagtcccccactttaacatcacgaatctcgatagctttgccattagtaactaccattgtatctaagctcaggcagcggttcagttgtgatgacccaccgccaccttgggccaacttcttcttcaactgcgccaattttttttttaaggcttgtaacttccacttcagttgggcaagcttttttttgtttgcttgcagcttcttttttaattgcgcacctcctttgcgtccacaaccacgttttccacctgtgctacagatcgaagaattatgcactacggtaccgcgtacattgtaggaatgatcttgattcacggtaaggtcgtggaccttccctttgtaatgcttcgtttcgatgcttgtgatttctttcagcttaaatttcatcgacccgcctcctcctgggccctggaaaagtacctcaagcgaccccccaccgccaatggcgacaacaagttcgtcgtcgtccgtaagctcatcagcacgaacatatccgcggttctttgtatacactggatggtctggcgtacaacgaattgttttcaccccgtgtaaagtgtcaatttccaattcaaccaattctgcgttcggacgcgtaagctgggcggctttgattggggcccactcgatgttgtccgtatgggtattgaatgcaagtacctggatatttttgtcattatcaaagatttcaactacttctttaatcgtagtctttccatagttgcgtgtaatgatttccgaagatccgactaaacaacaggggttcgttccgctcccgccgcccccgccacctttgcggccacagccacgtttgccaccttgtgcaagttccttttccaactgggcgttttccttctcaagtgcttgcagttcccactcaagttgcgcaagctccttttccaaggcctgaagctctttttccaactgggcactcccccctccgcctacatcggacgagcttgaattgtgcgtcaagatatcgttcgcataaaacaaatggttgccgctaacctcgatgtcgattaattcacgctcgtcaagttcttcaattttcagaattttcttaagcatcat igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z