BBa_K2140005
1
BBa_K2140005
StarScafold NonSequential 1
2016-10-12T11:00:00Z
2016-10-13T04:36:23Z
The Leucine Zipper, Linker and Cystine regions we're designed denovo from the literature, while The inteins are a combination of engineered and non-engineered genomic gene segments.
1. Stevens, A. J., Brown, Z. Z., Shah, N. H., Sekar, G., Cowburn, D., & Muir, T. W. (2016). Design of a Split Intein with Exceptional Protein Splicing Activity. Journal of the American Chemical Society, 138(7), 2162-2165.
2. Carvajal-Vallejos, P., Palliss??, R., Mootz, H. D., & Schmidt, S. R. (2012). Unprecedented rates and efficiencies revealed for new natural split inteins from metagenomic sources. Journal of Biological Chemistry, 287(34), 28686-28696.
3. Dassa, B., London, N., Stoddard, B. L., Schueler-Furman, O., & Pietrokovski, S. (2009). Fractured genes: a novel genomic arrangement involving new split inteins and a new homing endonuclease family. Nucleic acids research, gkp095.
Sequential intein activation is not required for enzyme splicing, we have a set of four previously tested inteins we can use for enzyme co-localisation which have been shown to have a high splicing rate, yield and orthologous splicing reactions. This part contains the coding sequence for our Non-Sequential StarScaffold, which allows us to check structural changes that occur due to splicing reactions occurring in the same mixture but at alternate temperatures.
false
false
_2610_
31465
31465
9
false
The length of the linker and leucine zipper regions along with the order of the intein segments.
false
Robert Ashley Naturani
annotation2497201
1
ATG (reverse compliment)
range2497201
1
1306
1308
BBa_K2140005_sequence
1
cccttgaaagtaaagattctccgaacccccacctcctttcgcacgagaacgcaagaaatctccgacttttaaaccgctattaatcgtttttaagccgtctttagtgggaaatttgtgattagcagagacgcggatttttttcccagatttactcttcaggacaatctcgaaaaccggttgtttgataatcggtaacacttccgtcacctgtacaggtccacactcgctttcaagccagtcccccactttaacatcacgaatctcgatagctttgccattagtaactaccattgtatctaagctcaggcagcggttcagttgtgatgacccaccgccaccttgggccaacttcttcttcaactgcgccaattttttttttaaggcttgtaacttccacttcagttgggcaagcttttttttgtttgcttgcagcttcttttttaattgcgcacctcctttgcgtccacaaccacgttttccacctgtgctacagatcgaagaattatgcactacggtaccgcgtacattgtaggaatgatcttgattcacggtaaggtcgtggaccttccctttgtaatgcttcgtttcgatgcttgtgatttctttcagcttaaatttcatcgacccgcctcctcctgggccctggaaaagtacctcaagcgaccccccaccgccaatggcgacaacaagttcgtcgtcgtccgtaagctcatcagcacgaacatatccgcggttctttgtatacactggatggtctggcgtacaacgaattgttttcaccccgtgtaaagtgtcaatttccaattcaaccaattctgcgttcggacgcgtaagctgggcggctttgattggggcccactcgatgttgtccgtatgggtattgaatgcaagtacctggatatttttgtcattatcaaagatttcaactacttctttaatcgtagtctttccatagttgcgtgtaatgatttccgaagatccgactaaacaacaggggttcgttccgctcccgccgcccccgccacctttgcggccacagccacgtttgccaccttgtgcaagttccttttccaactgggcgttttccttctcaagtgcttgcagttcccactcaagttgcgcaagctccttttccaaggcctgaagctctttttccaactgggcactcccccctccgcctacatcggacgagcttgaattgtgcgtcaagatatcgttcgcataaaacaaatggttgccgctaacctcgatgtcgattaattcacgctcgtcaagttcttcaattttcagaattttcttaagcatcat
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z