BBa_K214001 1 BBa_K214001 Pconst-RBS-Lpp-OmpA-Linker-TEV-TEV-Linker-ANP 2009-10-18T11:00:00Z 2015-05-08T01:11:29Z Combination of biobrick sequences Pconst(BBa_J23119) RBS (BBa_B0034), Lpp (BBa_J36835), OmpA (BBa_J36837), Linker (BBa_K157013-sequence between restriction sites), TEV cut site (BBa_K128002), ANP (consensus mature sequence, Genbank) ANP outer membrane expression construct, with twin TEV cut sites designed for release of adhesion. Over expression of sequence can be toxic in bacteria. false false _316_ 0 4135 9 It's complicated false Several restriction sites between domains to allow for future ease of modification. false QGEM_09 annotation2054697 1 Dual TEV cut site (K128002) range2054697 1 546 587 annotation2054699 1 Sal I range2054699 1 489 494 annotation2054703 1 RBS (B0034) range2054703 1 36 47 annotation2054694 1 Linker (K157013-modified) range2054694 1 594 638 annotation2054702 1 Hind III range2054702 1 48 53 annotation2054701 1 Lpp (J36835) range2054701 1 54 140 annotation2054700 1 OmpA (J36837) range2054700 1 147 488 annotation2054698 1 Linker (K157013-modified) range2054698 1 495 539 annotation2054695 1 BamHI range2054695 1 639 644 annotation2054693 1 Mlu I range2054693 1 588 593 annotation2054696 1 ANP (K214002) range2054696 1 645 731 annotation2054704 1 Xho I range2054704 1 540 545 annotation2054692 1 Pconst (J23119) range2054692 1 1 35 BBa_K214001_sequence 1 ttgacagctagctcagtcctaggtataatgctagcaaagaggagaaaaagcttatgaaagctactaaactggtactgggcgcggtaatcctgggttctactctgctggcaggttgctccagcaacgctaaaatcgatcagactagaaacccgtatgttggctttgaaatgggttacgactggttaggtcgtatgccgtacaaaggcagcgttgaaaacggtgcatacaaagctcagggcgttcaactgaccgctaaactgggttacccaatcactgacgacctggacatctacactcgtctgggtggcatggtatggcgtgcagacactaaatccaacgtttatggtaaaaaccacgacaccggcgtttctccggtcttcgctggcggtgttgagtacgcgatcactcctgaaatcgctacccgtctggaataccagtggaccaacaacatcggtgacgcacacaccatcggcactcgtccggacaacgtcgacggtggaggaggttctggaggcggtggaagtggtggcggaggtagcctcgaggaaaacctgtacttccagggtgaaaacctgtacttccagggtacgcgtggtggaggaggttctggaggcggtggaagtggtggcggaggtagcggatccagcctgcggagatccagctgcttcgggggcaggatggacaggattggagcccagagcggactgggctgtaacagcttccggtactaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z