BBa_K2141001
1
BBa_K2141001
Clover - alpha synuclein (1-60) - mRuby2 complex
2016-10-03T11:00:00Z
2016-10-29T07:47:08Z
Alpha-synuclein is encoded by SNCA gene in human. Clover and mRuby2 proteins are synthetic fluorescent proteins derived from classical fluorescent proteins.
Clover is a yellow-green fluorescent protein derived from avGFP with increased efficiency, approximately 1.5 to 3 times more efficient than classical GFP and YFP reporters. mRuby2 is also a fluorescent protein with a red emission colour. mRuby2 is 2.5 times brighter than another red fluorescent protein mCherry. Both Clover and mRuby2 increase the efficiency when used in a FRET (F??rster resonance energy transfer) system compared to classical fluorescent proteins such as GFP, YFP and RFP. Alpha-synuclein is a neural protein found in presynaptic terminals and responsible from dopamine tuning in the presynaptic terminals. Alpha-synuclein is also said to be involved with methamphetamine. Methamphetamine binds to alpha synuclein's N-terminal domain and causes a conformational change. This complex together will help to detect the presence of methamphetamine by using the conformational change in the alpha-synuclein protein and the FRET effect caused by Clover-mRuby2 fluorescent proteins.
false
false
_2611_
31310
31311
9
false
We used Clover and mRuby2 in order to increase the FRET effect and codon optimizations of all three sequences in order to produce the protein in E.coli with maximum efficiency.
false
Altug Uludag, Nedim Haciosmanoglu, Galip Can Guclu, Yagmur Guneri, Melis Akgun
annotation2485969
1
BamHI resctriction site
range2485969
1
1726
1731
annotation2485968
1
mRuby2
range2485968
1
1015
1725
annotation2485966
1
alpha synuclein (1-93)
range2485966
1
727
1005
annotation2485965
1
GGS linker
range2485965
1
718
726
annotation2485967
1
GGS linker
range2485967
1
1006
1014
annotation2485964
1
Clover
range2485964
1
1
717
BBa_K2141001_sequence
1
atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtccgcggcgagggcgagggcgatgccaccaacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcgtggcctgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatctctttcaaggacgacggtacctacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaaactggagtacaacttcaactctcacaacgtctatattaccgcagacaagcagaagaacggcattaaagctaacttcaagatccgccataacgtggaggacggttctgtacagctcgccgaccattaccagcagaacaccccgatcggcgacggccccgtactgctgcccgacaaccactacctgagccatcagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgcggccggtattacacatggcatggacgagctgtacaagggtggtagtatggatgtgtttatgaaaggcctgagcaaagcgaaagaaggcgtggttgccgcagccgaaaaaaccaaacagggcgtggcggaagcggcgggcaaaaccaaagaaggcgtgctgtatgttggcagcaaaaccaaagaaggcgtggtgcatggcgtggcgaccgttgcggaaaaaaccaaagaacaggtgaccaacgtgggcggcgcagttgtgaccggcgtgaccgcagtggcgcagaaaaccgtggaaggcgcaggcagcattgcggcagcgaccggcggtggtagtatggtgtctaagggcgaagagctgatcaaggaaaatatgcgtatgaaggttgtcatggaaggttcggtcaacggccaccaattcaaatgcacaggtgaaggcgaaggcaatccgtacatgggaactcaaaccatgaggatcaaagtcatcgagggaggacccctgccatttgcctttgacattcttgccacgtcgttcatgtatggcagccgtacttttatcaagtacccgaaaggcattcctgatttctttaaacagtcctttcctgagggttttacttgggaaagagttacgagatacgaagatggtggagtcgtcaccgtcatgcaggacaccagccttgaggatggctgtctcgtttaccacgtccaagtcagaggggtaaactttccctccaatggtcccgtgatgcagaagaagaccaagggttgggagcctaatacagagatgatgtatccagcagatggtggtctgaggggatacactcatatggcactgaaagttgatggtggtggccatctgtcttgctctttcgtaacaacttacaggtcaaaaaagaccgtcgggaacatcaagatgcccggtatccatgccgttgatcaccgcctggaaaggttagaggaaagtgacaatgaaatgttcgtagtacaacgcgaacacgcagttgccaagttcgccgggcttggtggtgggatggacgagctgtacaagggatcc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z