BBa_K2141001 1 BBa_K2141001 Clover - alpha synuclein (1-60) - mRuby2 complex 2016-10-03T11:00:00Z 2016-10-29T07:47:08Z Alpha-synuclein is encoded by SNCA gene in human. Clover and mRuby2 proteins are synthetic fluorescent proteins derived from classical fluorescent proteins. Clover is a yellow-green fluorescent protein derived from avGFP with increased efficiency, approximately 1.5 to 3 times more efficient than classical GFP and YFP reporters. mRuby2 is also a fluorescent protein with a red emission colour. mRuby2 is 2.5 times brighter than another red fluorescent protein mCherry. Both Clover and mRuby2 increase the efficiency when used in a FRET (F??rster resonance energy transfer) system compared to classical fluorescent proteins such as GFP, YFP and RFP. Alpha-synuclein is a neural protein found in presynaptic terminals and responsible from dopamine tuning in the presynaptic terminals. Alpha-synuclein is also said to be involved with methamphetamine. Methamphetamine binds to alpha synuclein's N-terminal domain and causes a conformational change. This complex together will help to detect the presence of methamphetamine by using the conformational change in the alpha-synuclein protein and the FRET effect caused by Clover-mRuby2 fluorescent proteins. false false _2611_ 31310 31311 9 false We used Clover and mRuby2 in order to increase the FRET effect and codon optimizations of all three sequences in order to produce the protein in E.coli with maximum efficiency. false Altug Uludag, Nedim Haciosmanoglu, Galip Can Guclu, Yagmur Guneri, Melis Akgun annotation2485969 1 BamHI resctriction site range2485969 1 1726 1731 annotation2485968 1 mRuby2 range2485968 1 1015 1725 annotation2485966 1 alpha synuclein (1-93) range2485966 1 727 1005 annotation2485965 1 GGS linker range2485965 1 718 726 annotation2485967 1 GGS linker range2485967 1 1006 1014 annotation2485964 1 Clover range2485964 1 1 717 BBa_K2141001_sequence 1 atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtccgcggcgagggcgagggcgatgccaccaacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcgtggcctgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatctctttcaaggacgacggtacctacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaaactggagtacaacttcaactctcacaacgtctatattaccgcagacaagcagaagaacggcattaaagctaacttcaagatccgccataacgtggaggacggttctgtacagctcgccgaccattaccagcagaacaccccgatcggcgacggccccgtactgctgcccgacaaccactacctgagccatcagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgcggccggtattacacatggcatggacgagctgtacaagggtggtagtatggatgtgtttatgaaaggcctgagcaaagcgaaagaaggcgtggttgccgcagccgaaaaaaccaaacagggcgtggcggaagcggcgggcaaaaccaaagaaggcgtgctgtatgttggcagcaaaaccaaagaaggcgtggtgcatggcgtggcgaccgttgcggaaaaaaccaaagaacaggtgaccaacgtgggcggcgcagttgtgaccggcgtgaccgcagtggcgcagaaaaccgtggaaggcgcaggcagcattgcggcagcgaccggcggtggtagtatggtgtctaagggcgaagagctgatcaaggaaaatatgcgtatgaaggttgtcatggaaggttcggtcaacggccaccaattcaaatgcacaggtgaaggcgaaggcaatccgtacatgggaactcaaaccatgaggatcaaagtcatcgagggaggacccctgccatttgcctttgacattcttgccacgtcgttcatgtatggcagccgtacttttatcaagtacccgaaaggcattcctgatttctttaaacagtcctttcctgagggttttacttgggaaagagttacgagatacgaagatggtggagtcgtcaccgtcatgcaggacaccagccttgaggatggctgtctcgtttaccacgtccaagtcagaggggtaaactttccctccaatggtcccgtgatgcagaagaagaccaagggttgggagcctaatacagagatgatgtatccagcagatggtggtctgaggggatacactcatatggcactgaaagttgatggtggtggccatctgtcttgctctttcgtaacaacttacaggtcaaaaaagaccgtcgggaacatcaagatgcccggtatccatgccgttgatcaccgcctggaaaggttagaggaaagtgacaatgaaatgttcgtagtacaacgcgaacacgcagttgccaagttcgccgggcttggtggtgggatggacgagctgtacaagggatcc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z