LOCUS       BBa_K2141002            2169 bp    DNA     linear       22-Dec-2024
DEFINITION  ECFP-meth scFv-EYFP
ACCESSION   BBa_K2141002
VERSION     BBa_K2141002.1  
FEATURES             Location/Qualifiers
     RBS             1..39
                     /label=RBS
     misc_feature    40..42
                     /label=ATG
     CDS             43..756
                     /label=ECFP
     CDS             757..1089
                     /label=antibody chain 1
     misc_feature    1090..1107
                     /label=linker
     CDS             1108..1458
                     /label=antibody chain 2
     CDS             1459..2166
                     /label=EYFP
     misc_feature    2167..2169
                     /label=stop codon
ORIGIN
        1 ctagaaataa ttttgtttaa ctttaagaag gagatataca tggtgagcaa gggcgaggag
       61 ctgttcaccg gggttgtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
      121 ttcagcgtgt ccggtgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
      181 atctgcacta ccggcaagct gcccgtgccc tggcccaccc tcgtgactac cctgacctgg
      241 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt tttcaagtcc
      301 gccatgcccg aaggctacgt tcaggagcgc accatcttct tcaaggacga cggcaactac
      361 aagacccgcg ccgaggtgaa gttcgagggt gacaccctgg ttaaccgcat cgagctgaag
      421 ggcatcgact tcaaggagga cggtaacatc ctgggtcata agctggagta caactacatc
      481 agccacaacg tgtatatcac cgccgacaag cagaagaacg gcatcaaggc gaacttcaag
      541 atccgccaca acatcgagga cggcagcgtt cagctcgccg accactacca gcagaacacc
      601 cccatcggcg acggccccgt tctgctgccc gacaaccact acctgagcac ccagtccgcc
      661 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
      721 gccgggatca ctctcggcat ggacgagctg tacaagagcc agattgttct gacccagagc
      781 ccggcgatta tgagcgcgag cccgggcgaa aaagtgaccc tgacctgtag cgccagctct
      841 agcgttagct ccagccatct gtattggtat cagcagaaac cgggcagcag cccgaaactg
      901 tggatttata gcaccagcaa cctggcgagc ggcgtgccgg cgcgctttag cggcagcggc
      961 agcggcacca gctatagcct gaccattagc agcatggaag cggaagatgc ggcgagctat
     1021 ttttgccatc agtggagcag ctttccgttt acctttggca gcggcaccaa actggaaatt
     1081 aaacgcgcgg gtggtggatc cggtggcgaa gtgcagctgc aagaaagcgg cccgagcctg
     1141 gtgaaaccga gccagaccct gagcctgacc tgttctgtga ccggcgatag cgtgacctct
     1201 ggctattgga gctggattcg ccagtttccg ggcaacaaac tggattatat gggctatatt
     1261 agctatcgcg gcagcaccta ctataacccg agcctgaaat ctcgcattag cattacccgc
     1321 gataccagca aaaaccaggt gtatctgcaa ctgaaaagcg tgagctctga agataccgcc
     1381 acctactatt gcagctactt cgattctgac gattacgcga tggaatattg gggccagggc
     1441 accagcgtga ccgtgagcgt gagcaagggc gaggagctgt tcaccggggt tgtgcccatc
     1501 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtctgg cgagggtgag
     1561 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
     1621 gtgccctggc ccaccctcgt gaccaccttc ggctacggcc tgcaatgctt cgcccgctac
     1681 cccgaccaca tgaagcagca cgacttcttt aagtccgcca tgcccgaagg ctacgtccag
     1741 gagcgcacca tcttctttaa ggacgatggc aactacaaga cccgcgccga ggtgaagttc
     1801 gagggtgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
     1861 aacatcctgg ggcacaagct ggagtataac tacaacagcc acaacgtcta tatcatggcc
     1921 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc
     1981 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     2041 ctgcccgaca accactacct gagctaccag tccgccctga gcaaagaccc caacgagaag
     2101 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
     2161 gagctgtaa
//