LOCUS       BBa_K2141003            2240 bp    DNA     linear       22-Nov-2024
DEFINITION  yfp-bicistronic anti-meth antibody-cfp
ACCESSION   BBa_K2141003
VERSION     BBa_K2141003.1  
FEATURES             Location/Qualifiers
     RBS             1..39
                     /label=RBS
     misc_feature    40..42
                     /label=ATG
     CDS             44..705
                     /label=YFP
     misc_feature    706..717
                     /label=GS linker
     misc_feature    718..723
                     /label=SacI restriction site
     CDS             724..1053
                     /label=C1
     misc_feature    1054..1056
                     /label=Stop codon
     misc_feature    1057..1062
                     /label=XhoI restriction site
     promoter        1063..1114
                     /label=T7 promoter
     RBS             1115..1154
                     /label=RBS
     misc_feature    1155..1157
                     /label=ATG
     CDS             1158..1508
                     /label=C2
     misc_feature    1509..1520
                     /label=GS linker
     misc_feature    1521..1526
                     /label=AclI restriction site
     CDS             1527..2237
                     /label=CFP
     misc_feature    2238..2240
                     /label=Stop codon
ORIGIN
        1 ctagaaataa ttttgtttaa ctttaagaag gagatataca tgaccggcgt ggtgccgatt
       61 ctggtggaac tggatggcga tgtgaacggc cataaattta gcgtgagcgg cgaaggcgaa
      121 ggcgatgcga cctatggcaa actgaccctg aaactgattt gcaccaccgg caaactgccg
      181 gtgccgtttc cgaccctggt gaccaccctg cttcagtgct ttgcgcgcta tccggatcat
      241 atgaaacagc atgatttctt caaaagcgcg atgccggaag gctatgtgca ggaacgcacc
      301 attttcttca aagatgatgg caactataaa acccgcgcgg aagtgaaatt tgaaggcgat
      361 accctggtga accgcattga actgaaaggc attgatttca aagaagatgg caacattctg
      421 ggccataaac tggaatataa ctataacagc cataacgtgt atattaccgc ggataaacag
      481 aaaaacggca ttaaagcgaa cttcaaaatt cgccataaca ttgaagatgg cggcgtgcag
      541 ctggcggatc attatcagca gaacaccccg attggcgatg gcccggtgct gctgccggat
      601 aaccattatc tgagctatca gagcaaactg agcaaagatc cgaacgaaaa acgcgatcat
      661 atggtgctgc tggaatttgt gaccgcggcg ggcggcagca gcagcggcgg cggctcggag
      721 ctccagattg tgctgaccca gagcccggcg attatgagcg cgagcccggg cgaaaaagtg
      781 accctgacct gtagcgcgag cagcagcgtg agcagcagcc atctgtattg gtatcagcag
      841 aaaccgggca gcagcccgaa actgtggatt tatagcacca gcaacctggc gagcggcgtg
      901 ccggcgcgct ttagcggcag cggcagcggc accagctata gcctgaccat tagcagcatg
      961 gaagcggaag atgcggcgag ctatttttgc catcagtgga gcagctttcc gtttaccttt
     1021 ggcagcggca ccaaactgga aattaaacgc gcgtaactcg aggcgtagag gatcgagatc
     1081 tcgatcccgc gaaattaata cgactcacta taggctagaa ataattttgt ttaactttaa
     1141 gaaggagata taccatggaa gtgcagctgc aagaaagcgg cccgagcctg gtgaaaccga
     1201 gccagaccct gagcctgacc tgtagtgtga ccggcgatag cgtgaccagc ggctattgga
     1261 gctggattcg ccagtttccg ggcaacaaac tggattatat gggctatatt agctatcgcg
     1321 gcagcaccta ttataacccg agcctgaaaa gccgcattag cattacccgc gataccagca
     1381 aaaaccaggt gtatctgcaa ctgaaaagcg tgagcagcga agataccgcg acctattatt
     1441 gcagctattt tgatagcgat gattatgcga tggaatattg gggccagggc accagcgtga
     1501 ccgtgagcgg cggcggctcg aacgttatgg tgagcaaagg cgaagaactg tttaccggcg
     1561 tggtgccgat tctggtggaa ctggatggcg atgtgaacgg ccataaattc agcgtgtctg
     1621 gcgaaggcga aggcgatgcg acctatggca aactgaccct gaaatttatt tgcacgaccg
     1681 gcaaactgcc ggtgccgtgg ccgaccctgg tgacgaccct gggcgtgcag tgctttgcgc
     1741 gctatccgga tcatatgaaa cagcatgatt tcttcaaaag cgcgatgccg gaaggctatg
     1801 tgcaggaacg caccattttc ttcaaagatg atggcaacta taaaacccgc gcggaagtga
     1861 aatttgaagg cgataccctg gtgaaccgca ttgaactgaa aggcattgat tttaaagaag
     1921 atggcaacat tctgggccat aaactggaat ataactatat tagcggcaac gtgtatatta
     1981 ccgcggataa acagaaaaac ggcattaaag cgaacttcaa aattcgccat aacattgaag
     2041 atggtggcgt gcagctggcg gatcattatc agcagaacac cccgattggc gatggcccgg
     2101 tgctgctgcc ggataaccac tatctgagca cccagagcgc gctgagcaaa gatccgaacg
     2161 aaaaacgcga tcatatggtg ctgctggaat ttctgaccgc cgcgggcatt accctgggca
     2221 tggatgaact gtataaataa
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