BBa_K2141003 1 BBa_K2141003 yfp-bicistronic anti-meth antibody-cfp 2016-10-13T11:00:00Z 2016-10-14T05:02:45Z It is ordered via IDT sponsorship. Anti-meth scFv is coming from mus musculus. YFP and CFP are the common fluorescent proteins which are derivatives of GFP. This construct consists one domain of anti-meth scFv antibody (termed as c1 in the project) conjugated with YFP, and the other domain of anti-meth scFv antibody (termed as c2 in the project) conjugated with CFP. Also, the construct has its own T7 promoter sequence in addition to the T7 promoter in the vector it is cloned into. This design constructed with the thought of separately producing the two domains of the anti-meth antibody which are conjugated with fluorescence proteins YFP and CFP, when the antibody domains came together in the presence of methamphetamine, it will give a FRET (f??rster resonance energy transfer) emission between CFP and YFP. false false _2611_ 31311 31311 9 false Bicistronic construct is designed to obtain a FRET effect by the approach of two domain towards each other in the presence of methamphetamine false Altug Uludag, Nedim Haciosmanoglu, Galip Can Guclu, Yagmur Guneri, Melis Akgun annotation2509989 1 ATG range2509989 1 40 42 annotation2509993 1 C1 range2509993 1 724 1053 annotation2510050 1 Stop codon range2510050 1 2238 2240 annotation2509988 1 RBS range2509988 1 1 39 annotation2509997 1 RBS range2509997 1 1115 1154 annotation2510016 1 C2 range2510016 1 1158 1508 annotation2510040 1 CFP range2510040 1 1527 2237 annotation2509996 1 T7 promoter range2509996 1 1063 1114 annotation2509992 1 SacI restriction site range2509992 1 718 723 annotation2510017 1 GS linker range2510017 1 1509 1520 annotation2509990 1 YFP range2509990 1 44 705 annotation2510018 1 AclI restriction site range2510018 1 1521 1526 annotation2509994 1 Stop codon range2509994 1 1054 1056 annotation2510015 1 ATG range2510015 1 1155 1157 annotation2509995 1 XhoI restriction site range2509995 1 1057 1062 annotation2509991 1 GS linker range2509991 1 706 717 BBa_K2141003_sequence 1 ctagaaataattttgtttaactttaagaaggagatatacatgaccggcgtggtgccgattctggtggaactggatggcgatgtgaacggccataaatttagcgtgagcggcgaaggcgaaggcgatgcgacctatggcaaactgaccctgaaactgatttgcaccaccggcaaactgccggtgccgtttccgaccctggtgaccaccctgcttcagtgctttgcgcgctatccggatcatatgaaacagcatgatttcttcaaaagcgcgatgccggaaggctatgtgcaggaacgcaccattttcttcaaagatgatggcaactataaaacccgcgcggaagtgaaatttgaaggcgataccctggtgaaccgcattgaactgaaaggcattgatttcaaagaagatggcaacattctgggccataaactggaatataactataacagccataacgtgtatattaccgcggataaacagaaaaacggcattaaagcgaacttcaaaattcgccataacattgaagatggcggcgtgcagctggcggatcattatcagcagaacaccccgattggcgatggcccggtgctgctgccggataaccattatctgagctatcagagcaaactgagcaaagatccgaacgaaaaacgcgatcatatggtgctgctggaatttgtgaccgcggcgggcggcagcagcagcggcggcggctcggagctccagattgtgctgacccagagcccggcgattatgagcgcgagcccgggcgaaaaagtgaccctgacctgtagcgcgagcagcagcgtgagcagcagccatctgtattggtatcagcagaaaccgggcagcagcccgaaactgtggatttatagcaccagcaacctggcgagcggcgtgccggcgcgctttagcggcagcggcagcggcaccagctatagcctgaccattagcagcatggaagcggaagatgcggcgagctatttttgccatcagtggagcagctttccgtttacctttggcagcggcaccaaactggaaattaaacgcgcgtaactcgaggcgtagaggatcgagatctcgatcccgcgaaattaatacgactcactataggctagaaataattttgtttaactttaagaaggagatataccatggaagtgcagctgcaagaaagcggcccgagcctggtgaaaccgagccagaccctgagcctgacctgtagtgtgaccggcgatagcgtgaccagcggctattggagctggattcgccagtttccgggcaacaaactggattatatgggctatattagctatcgcggcagcacctattataacccgagcctgaaaagccgcattagcattacccgcgataccagcaaaaaccaggtgtatctgcaactgaaaagcgtgagcagcgaagataccgcgacctattattgcagctattttgatagcgatgattatgcgatggaatattggggccagggcaccagcgtgaccgtgagcggcggcggctcgaacgttatggtgagcaaaggcgaagaactgtttaccggcgtggtgccgattctggtggaactggatggcgatgtgaacggccataaattcagcgtgtctggcgaaggcgaaggcgatgcgacctatggcaaactgaccctgaaatttatttgcacgaccggcaaactgccggtgccgtggccgaccctggtgacgaccctgggcgtgcagtgctttgcgcgctatccggatcatatgaaacagcatgatttcttcaaaagcgcgatgccggaaggctatgtgcaggaacgcaccattttcttcaaagatgatggcaactataaaacccgcgcggaagtgaaatttgaaggcgataccctggtgaaccgcattgaactgaaaggcattgattttaaagaagatggcaacattctgggccataaactggaatataactatattagcggcaacgtgtatattaccgcggataaacagaaaaacggcattaaagcgaacttcaaaattcgccataacattgaagatggtggcgtgcagctggcggatcattatcagcagaacaccccgattggcgatggcccggtgctgctgccggataaccactatctgagcacccagagcgcgctgagcaaagatccgaacgaaaaacgcgatcatatggtgctgctggaatttctgaccgccgcgggcattaccctgggcatggatgaactgtataaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z