LOCUS       BBa_K2142005            2338 bp    DNA     linear       26-Dec-2024
DEFINITION  Composite part containing inducible mazF toxin  and dam metylase
ACCESSION   BBa_K2142005
VERSION     BBa_K2142005.1  
FEATURES             Location/Qualifiers
     misc_feature    1..3
                     /label=stop
     CDS             1..1084
                     /label=LacI
     misc_feature    1081..1084
                     /label=start
     RBS             1085..1092
                     /label=rbs
     promoter        1093..1153
                     /label=LacIq promoter
     misc_feature    1..1153
                     /label=BBa_K731300
     misc_feature    1194..1219
                     /label=lacO operator
     promoter        1162..1188
                     /label=Ptac promoter
     promoter        1..1239
                     /label=BBa_K731500
     RBS             1248..1259
                     /label=BBa_J61100
     CDS             1266..1475
                     /label=BBa_K2142003
     RBS             1484..1495
                     /label=BBa_J61100
     CDS             1502..2338
                     /label=BBa_K2142004
ORIGIN
        1 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
       61 cgcgcgggga gaggcggttt gcgtattggg cgccagggtg gtttttcttt tcaccagtga
      121 gacgggcaac agctgattgc ccttcaccgc ctggccctga gagagttgca gcaagcggtc
      181 cacgctggtt tgccccagca ggcgaaaatc ctgtttgatg gtggttaacg gcgggatata
      241 acatgagctg tcttcggtat cgtcgtatcc cactaccgag atatccgcac caacgcgcag
      301 cccggactcg gtaatggcgc gcattgcgcc cagcgccatc tgatcgttgg caaccagcat
      361 cgcagtggga acgatgccct cattcagcat ttgcatggtt tgttgaaaac cggacatggc
      421 actccagtcg ccttcccgtt ccgctatcgg ctgaatttga ttgcgagtga gatatttatg
      481 ccagccagcc agacgcagac gcgccgagac agaacttaat gggcccgcta acagcgcgat
      541 ttgctggtga cccaatgcga ccagatgctc cacgcccagt cgcgtaccgt cttcatggga
      601 gaaaataata ctgttgatgg gtgtctggtc agagacatca agaaataacg ccggaacatt
      661 agtgcaggca gcttccacag caatggcatc ctggtcatcc agcggatagt taatgatcag
      721 cccactgacg cgttgcgcga gaagattgtg caccgccgct ttacaggctt cgacgccgct
      781 tcgttctacc atcgacacca ccacgctggc acccagttga tcggcgcgag atttaatcgc
      841 cgcgacaatt tgcgacggcg cgtgcagggc cagactggag gtggcaacgc caatcagcaa
      901 cgactgtttg cccgccagtt gttgtgccac gcggttggga atgtaattca gctccgccat
      961 cgccgcttcc actttttccc gcgttttcgc agaaacgtgg ctggcctggt tcaccacgcg
     1021 ggaaacggtc tgataagaga caccggcata ctctgcgaca tcgtataacg ttactggttt
     1081 cacattcacc accctgaatt gactctcttc cgggcgctat catgccatac cgcgaaaggt
     1141 tttgcgccat tctactagag gttgacaatt aatcatcggc tcgtataatg tgtgggaatt
     1201 gtgagcggat aacaattccc tcatgaaata attttgtttt actagagaaa gaggggacat
     1261 actagatggt aagccgatac gtacccgata tgggcgatct gatttgggtt gattttgacc
     1321 cgacaaaagg tagcgagcaa gctggacatc gtccagctgt tgtcctgagt cctttcatgt
     1381 acaacaacaa aacaggtatg tgtctgtgtg ttccttatac aacgcaatca aaaggatatc
     1441 cgttcgaagt tgttttatcc ggtcaggaaa cgtgatacta gagaaagagg ggacatacta
     1501 gatgaagaaa aatcgcgctt ttttgaagtg ggcagggggc aagtatcccc tgcttgatga
     1561 tattaaacgg catttgccca agggcgaatg tctggttgag ccttttgtag gtgccgggtc
     1621 ggtgtttctc aacaccgact tttctcgtta tatccttgcc gatatcaata gcgacctgat
     1681 cagtctctat aacattgtga agatgcgtac tgatgagtac gtacaggccg cacgcgagct
     1741 gtttgttccc gaaacaaatt gcgccgaggt ttactatcag ttccgcgaag agttcaacaa
     1801 aagccaggat ccgttccgtc gggcggtact gtttttatat ttgaaccgct acggttacaa
     1861 cggcctgtgt cgttacaatc tgcgcggtga gtttaacgtg ccgttcggcc gctacaaaaa
     1921 accctatttc ccggaagcag agttgtatca cttcgctgaa aaagcgcaga atgccttttt
     1981 ctattgtgag tcttacgccg atagcatggc gcgcgcagat gatgcatccg tcgtctattg
     2041 cgatccgcct tatgcaccgc tgtctgcgac cgccaacttt acggcgtatc acacaaacag
     2101 ttttacgctt gaacaacaag cgcatctggc ggagatcgcc gaaggtctgg ttgagcgcca
     2161 tattccagtg ctgatctcca atcacgatac gatgttaacg cgtgagtggt atcagcgcgc
     2221 aaaattgcat gtcgtcaaag ttcgacgcag tataagcagc aacggcggca cacgtaaaaa
     2281 ggtggacgaa ctgctggctt tgtacaaacc aggagtcgtt tcacccgcga aaaaataa
//