BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K2143001 1 BBa_K2143001 NahR_L154F Transcription Factor 2016-10-14T11:00:00Z 2016-10-15T11:42:45Z Pseudomonas putida Nah7 Plasmid Allele of NahR transcription factor that is able to induce the promoter Psal in the absence of Salicylate. false false _2613_ 30588 30588 9 false Optimized for use in E. coli. false Bryan Pasqualucci annotation2512428 1 NahR_L154F range2512428 1 1 903 BBa_K2143000 1 BBa_K2143000 NahR_L154F constitutive transcription factor binds upstream of Psal 2016-10-11T11:00:00Z 2016-10-15T11:44:32Z NCBI Reference Sequence: NC_007926.1 Codes for a mutant version of NahR transcription factor that binds upstream of Psal promoter. Wild Type version requires salicylate to induce Psal. This allele can induce Psal in the absence of salicylate. This part is useful to create a positive feedback loop when place downstream of Psal. false false _2613_ 30588 30588 9 false This part contains two copies of NahR_L154F. We did this because because we are using it as a positive feedback loop, we wanted the concentration of this mutant to be greater than the concentration of the inducer required allele. false Bryan Pasqualucci component2512440 1 BBa_B0034 component2512434 1 BBa_K2143001 component2512438 1 BBa_K2143001 component2512436 1 BBa_B0034 annotation2512436 1 BBa_B0034 range2512436 1 912 923 annotation2512434 1 BBa_K2143001 range2512434 1 1 903 annotation2512438 1 BBa_K2143001 range2512438 1 930 1832 annotation2512440 1 BBa_B0034 range2512440 1 1841 1852 BBa_K2143001_sequence 1 atggagttacgtgacttagatttgaacttacttgttgtctttaaccaattacttgtagatcgccgcgtgtccatcactgcggagaacttagggttaactcaaccagcggtatcaaacgcccttaaacgcctgcgcacctcactgcaagatcccctgtttgtccgtacccaccagggcatggaaccgacgccatatgccgctcatttggctgagcccgtaacttcggctatgcacgcattacgcaatgcgcttcaacaccacgaaagttttgaccctttaacatccgagcgtactttcacattagccatgacagacatcggtgagatttattttatgccccgtcttatggatgtccttgcacatcaagcaccgaactgcgtcatcagtactgtgcgcgatagttccatgtcgttaatgcaagcactgcaaaacgggacagtcgatcttgccgtcggcttgtttcctaatcttcagacgggattttttcagcgtcgcttattacagaatcattatgtatgcttatgtcgcaaggatcatcctgtcacccgcgaaccccttacgctggaacgcttttgttcctacggtcatgtacgtgttatcgcagcgggaacaggccacggggaagtggatacatacatgacacgcgtgggtattcgtcgcgatattcgcttggaggttccccacttcgctgccgtagggcacatcttacaacgcaccgatctgcttgctacagttcctatccacttagccgactgttgcgtggagccattcggcctttctgctcttccacatcccgtagtattgccggagatcgcgattaatatgttttggcatgcgaaataccataaagaccttgcaaatatctggttacgtcagctgatgtttgatttattcaccgactga BBa_B0034_sequence 1 aaagaggagaaa BBa_K2143000_sequence 1 atggagttacgtgacttagatttgaacttacttgttgtctttaaccaattacttgtagatcgccgcgtgtccatcactgcggagaacttagggttaactcaaccagcggtatcaaacgcccttaaacgcctgcgcacctcactgcaagatcccctgtttgtccgtacccaccagggcatggaaccgacgccatatgccgctcatttggctgagcccgtaacttcggctatgcacgcattacgcaatgcgcttcaacaccacgaaagttttgaccctttaacatccgagcgtactttcacattagccatgacagacatcggtgagatttattttatgccccgtcttatggatgtccttgcacatcaagcaccgaactgcgtcatcagtactgtgcgcgatagttccatgtcgttaatgcaagcactgcaaaacgggacagtcgatcttgccgtcggcttgtttcctaatcttcagacgggattttttcagcgtcgcttattacagaatcattatgtatgcttatgtcgcaaggatcatcctgtcacccgcgaaccccttacgctggaacgcttttgttcctacggtcatgtacgtgttatcgcagcgggaacaggccacggggaagtggatacatacatgacacgcgtgggtattcgtcgcgatattcgcttggaggttccccacttcgctgccgtagggcacatcttacaacgcaccgatctgcttgctacagttcctatccacttagccgactgttgcgtggagccattcggcctttctgctcttccacatcccgtagtattgccggagatcgcgattaatatgttttggcatgcgaaataccataaagaccttgcaaatatctggttacgtcagctgatgtttgatttattcaccgactgatactagagaaagaggagaaatactagatggagttacgtgacttagatttgaacttacttgttgtctttaaccaattacttgtagatcgccgcgtgtccatcactgcggagaacttagggttaactcaaccagcggtatcaaacgcccttaaacgcctgcgcacctcactgcaagatcccctgtttgtccgtacccaccagggcatggaaccgacgccatatgccgctcatttggctgagcccgtaacttcggctatgcacgcattacgcaatgcgcttcaacaccacgaaagttttgaccctttaacatccgagcgtactttcacattagccatgacagacatcggtgagatttattttatgccccgtcttatggatgtccttgcacatcaagcaccgaactgcgtcatcagtactgtgcgcgatagttccatgtcgttaatgcaagcactgcaaaacgggacagtcgatcttgccgtcggcttgtttcctaatcttcagacgggattttttcagcgtcgcttattacagaatcattatgtatgcttatgtcgcaaggatcatcctgtcacccgcgaaccccttacgctggaacgcttttgttcctacggtcatgtacgtgttatcgcagcgggaacaggccacggggaagtggatacatacatgacacgcgtgggtattcgtcgcgatattcgcttggaggttccccacttcgctgccgtagggcacatcttacaacgcaccgatctgcttgctacagttcctatccacttagccgactgttgcgtggagccattcggcctttctgctcttccacatcccgtagtattgccggagatcgcgattaatatgttttggcatgcgaaataccataaagaccttgcaaatatctggttacgtcagctgatgtttgatttattcaccgactgatactagagaaagaggagaaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z