LOCUS       BBa_K2144004             698 bp    DNA     linear       17-May-2024
DEFINITION  Coding sequence for Nuclease with His6 and LPXTG tag regulated by 
            T7-promoter
ACCESSION   BBa_K2144004
VERSION     BBa_K2144004.1  
FEATURES             Location/Qualifiers
     misc_feature    21..32
                     /label=B0034
     promoter        1..20
                     /label=T7 promoter
     RBS             24..27
                     /label=RBS
     promoter        1..32
                     /label=BBa_K525998
     misc_feature    61..72
                     /label=B0034
     promoter        41..60
                     /label=T7 promoter
     RBS             64..67
                     /label=RBS
     promoter        41..72
                     /label=BBa_K525998
     CDS             79..639
                     /label=BBa_K729004
     CDS             41..639
                     /label=BBa_K2144000
     misc_feature    648..661
                     /label=Linker
     misc_feature    662..678
                     /label=LPXTG
     misc_feature    679..695
                     /label=His6
     misc_feature    648..698
                     /label=BBa_K2144009
ORIGIN
        1 taatacgact cactataggg aaagaggaga aatactagag taatacgact cactataggg
       61 aaagaggaga aatactagat gaaaaagatt tggctggcgc tggctggttt agttttagcg
      121 tttagcgcat cggcgcaaac agataacggc gtaaatagaa gtggttctga agatccaaca
      181 gtatatagtg caacttcaac taaaaaatta cataaagaac ctgcgacttt aattaaagcg
      241 attgatggtg atacggttaa attaatgtac aaaggtcaac caatgacatt cagactatta
      301 ttggttgata cacctgaaac aaagcatcct aaaaaaggtg tagagaaata tggtcctgaa
      361 gcaagtgcat ttacgaaaaa aatggtagaa aatgcaaaga aaattgaagt cgagtttgac
      421 aaaggtcaaa gaactgataa atatggacgt ggcttagcgt atatttatgc tgatggaaaa
      481 atggtaaacg aagctttagt tcgtcaaggc ttggctaaag ttgcttatgt ttacaaacct
      541 aacaatacac atgaacaaca tttaagaaaa agtgaagcac aagcgaaaaa agagaaatta
      601 aatatttgga gcgaagacaa cgctgattca ggtcaataat actagagggc ggcggcggca
      661 gccttcctga aactggcggc caccaccacc accaccac
//