LOCUS       BBa_K2144005             838 bp    DNA     linear       22-Dec-2024
DEFINITION  Coding sequence for trunctated Lysostaphin with His6 and LPXTG-tag 
            regulated by T7-promoter
ACCESSION   BBa_K2144005
VERSION     BBa_K2144005.1  
FEATURES             Location/Qualifiers
     misc_feature    21..32
                     /label=B0034
     promoter        1..20
                     /label=T7 promoter
     RBS             24..27
                     /label=RBS
     promoter        1..32
                     /label=BBa_K525998
     CDS             39..779
                     /label=BBa_K748002
     CDS             1..779
                     /label=BBa_K2144001
     misc_feature    788..801
                     /label=Linker
     misc_feature    802..818
                     /label=LPXTG
     misc_feature    819..835
                     /label=His6
     misc_feature    788..838
                     /label=BBa_K2144009
ORIGIN
        1 taatacgact cactataggg aaagaggaga aatactagat gacacatgaa cattcagcac
       61 aatggttgaa taattacaaa aaaggatatg gttacggtcc ttatccatta ggtataaatg
      121 gcggtatgca ctacggagtt gattttttta tgaatattgg aacaccagta aaagctattt
      181 caagcggaaa aatagttgaa gctggttgga gtaattacgg aggaggtaat caaataggtc
      241 ttattgaaaa tgatggagtg catagacaat ggtatatgca tctaagtaaa tataatgtta
      301 aagtaggaga ttatgtcaaa gctggtcaaa taatcggttg gtctggaagc actggttatt
      361 ctacagcacc acatttacac ttccaaagaa tggttaattc attttcaaat tcaactgccc
      421 aagatccaat gcctttctta aagagcgcag gatatggaaa agcaggtggt acagtaactc
      481 caacgccgaa tacaggttgg aaaacaaaca aatatggcac actatataaa tcagagtcag
      541 ctagcttcac acctaataca gatataataa caagaacgac tggtccattt agaagcatgc
      601 cgcagtcagg agtcttaaaa gcaggtcaaa caattcatta tgatgaagtg atgaaacaag
      661 acggtcatgt ttgggtaggt tatacaggta acagtggcca acgtatttac ttgcctgtaa
      721 gaacatggaa taaatctact aatactttag gtgttctttg gggaactata aagtaataat
      781 actagagggc ggcggcggca gccttcctga aactggcggc caccaccacc accaccac
//