LOCUS       BBa_K2144006            1495 bp    DNA     linear       23-Dec-2024
DEFINITION  Coding sequence for ESP with His6 and LPXTG-tag regulated by 
            T7-promoter
ACCESSION   BBa_K2144006
VERSION     BBa_K2144006.1  
FEATURES             Location/Qualifiers
     misc_feature    21..32
                     /label=B0034
     promoter        1..20
                     /label=T7 promoter
     RBS             24..27
                     /label=RBS
     promoter        1..32
                     /label=BBa_K525998
     misc_feature    205..1037
                     /label=BBa_K531003
     misc_feature    1046..1405
                     /label=BBa_K531001
     misc_feature    41..1436
                     /label=BBa_K531005
     CDS             1..1436
                     /label=BBa_K2144002
     misc_feature    1445..1458
                     /label=Linker
     misc_feature    1459..1475
                     /label=LPXTG
     misc_feature    1476..1492
                     /label=His6
     misc_feature    1445..1495
                     /label=BBa_K2144009
ORIGIN
        1 taatacgact cactataggg aaagaggaga aatactagag gcggcttcta gcatggaccg
       61 cccgcgcccg tgaggccgag gatttcgcgc tggtcagaca acctacttgc cgtccccaca
      121 tgttagcgct accaagtgcc gacgaacgcg cgccgccgac ggtgtcggcg cttcagacgc
      181 tcgagttttg gggagaaata ctagatgaag aagcgcttcc tgtcgatctg caccatgacc
      241 atcgccgccc tggccacgac gaccatggtg aacacgagct atgcgaagac cgacaccgag
      301 tcccacaacc actcgtcgct gggtaccgaa aacaagaatg tcctggacat caacagcagc
      361 tcccacaaca tcaagccgag ccagaacaag agctatccga gcgtgatcct gccgaataac
      421 aaccgccacc agatcttcaa cacgacccaa gggcactatg acgccgtgtc gttcatctac
      481 atcccgatcg acggcggcta catcccgatc gacggcggct acatgagcgg ctccggcgtc
      541 gtcgtcggcg aaacgagatc ctcacgaata agcacgtcgt gaacggcgcg aagggcaacc
      601 cccggaatat ctccgtccat ccctcggcca agaacgagaa cgattatccc aatggtaagt
      661 tcgtgggcca agaaatcatc ccgtaccccg gtaactcgga cctggccatc ctgcgcgtga
      721 gccccaacga gcacaatcaa cacatcggcc aggtggtcaa gcccgcgacg atcagcagca
      781 acacggacac ccgcatcaac gagaacatca ccgtcaccgg ctaccccggc gacaagcccc
      841 tggcgaccat gtgggagagc gtcggcaagg tggtctatat cggcggcgag gagctgcgct
      901 atgacctcag cacggtgggc ggcaacagcg gctcgcccgt cttcaacggc aagaaccagg
      961 tgatcggcat ccactatggt ggtgtcgaca acaagtataa ctcgagcgtc tacatcaatg
     1021 acttcgtgca gcaattcctg cgcaacaaca tcccggacat caacatccag actagagata
     1081 tcaacgctat cggcacctcg accgctttcg tgacgatcac cgacgccgct gtcggcgaca
     1141 agctcgacct cgtcggcatc tcgacgaacg gcgctatcgc tgacggcgcc ttcggcgctg
     1201 cggtcaccct gggcgctgct gcgaccctgg ctcagtacct ggacgctgct gctgccggcg
     1261 acggcagcgg cacctcggtt gccaagtggt tccagttcgg cggcgacacc tatgtcgtcg
     1321 ttgacagctc ggctggcgcg accttcgtca gcggcgctga cgcggtgatc aagctgaccg
     1381 gtctggtcac gctgaccacc tcggccttcg ccaccgaagt cctgacgctc gcctaatact
     1441 agagggcggc ggcggcagcc ttcctgaaac tggcggccac caccaccacc accac
//