BBa_K2144006
1
BBa_K2144006
Coding sequence for ESP with His6 and LPXTG-tag regulated by T7-promoter
2016-10-11T11:00:00Z
2016-10-12T03:20:01Z
-
Esp, extracellular serine protease, is capable of inhibit the biofilm formation and nasal colonization of S. aureus. This by protelysing receptors essential for biofilm formation and prevention of eDNA exudation.
The inserted LT-sequence contain the gene encoding for His-tag which enables the use of IMAC to purify the combat protein. Furthermore it contains the LXPTG-tag which enables the use of the "match making" enzyme Sortase. The key feature of sortase is the specific conjugation reaction it carries out, where the enzyme recognizes a specific amino acid sequence, a so called sorting motif (LPXTG motif in the case of S.aureus) and conjugate this sequence with another unit carrying an oligo glycine motif. A new peptide bond is formed. The Linker in the LT-sequence enables proper folding of the protein.
false
false
_2614_
31517
31517
9
false
The LT-sequence was inserted with Overhang PCR.
false
Oskar ??hman
component2493557
1
BBa_K2144002
component2493561
1
BBa_K2144009
annotation2493561
1
BBa_K2144009
range2493561
1
1445
1495
annotation2493557
1
BBa_K2144002
range2493557
1
1
1436
BBa_K2144009
1
BBa_K2144009
Sequence for His6-tag and LPXTGG-tag
2016-10-11T11:00:00Z
2016-10-12T02:35:45Z
Coming soon
The His6-tag enables purification of proteins with IMAC. The LPXTG-tag enables the use of the "match making" enzyme sortase. The key feature of sortasee is the specific conjugation reaction it carries out, where the enzyme recognizes a specific amino acid sequence, a so called sorting motif (LPXTG motif in the case of S.aureus) and conjugate this sequence with another unit carrying an oligo glycine motif.
false
false
_2614_
31517
31517
9
false
Coming soon
false
Oskar ??hman
annotation2493495
1
His6
range2493495
1
32
48
annotation2493493
1
Linker
range2493493
1
1
14
annotation2493494
1
LPXTG
range2493494
1
15
31
BBa_K2144002
1
BBa_K2144002
ESP capable of inhibiting biofilm
2016-10-11T11:00:00Z
2016-10-12T01:57:08Z
Coming soon.
Esp, extracellular serine protease, is capable of inhibit the biofilm formation and nasal colonization of S. aureus. This by protelysing receptors essential for biofilm formation and prevention of eDNA exudation.
false
false
_2614_
31517
31517
9
false
Basic 3A Assembly was performed to make this BioBrick
false
Oskar ??hman
component2493465
1
BBa_K525998
component2493468
1
BBa_K531005
annotation2493465
1
BBa_K525998
range2493465
1
1
32
annotation2493468
1
BBa_K531005
range2493468
1
41
1436
BBa_K525998
1
pT7
Promoter T7 and RBS
2011-09-12T11:00:00Z
2015-05-08T01:12:35Z
Synthesis
Released HQ 2013
Promoter T7 and RBS
false
false
_690_
0
8543
9
In stock
false
Synthesis
false
Anna Drong
annotation2129433
1
RBS
range2129433
1
24
27
annotation2129432
1
T7 promoter
range2129432
1
1
20
annotation2127788
1
B0034
range2127788
1
21
32
BBa_K531005
1
BBa_K531005
P<sub>xyl</sub> promoter+optimized <i>esp</i>+<i>rsaA</i> secretion tag
2011-07-25T11:00:00Z
2015-05-08T01:12:36Z
From BBa_K531001 BBa_K531002 BBa_K531003
The BioBrick part enables C. crescentus to express and secret Esp protein in the presence of xylose.
false
false
_697_
0
8860
9
In stock
false
no stop codon in esp gene and no start codon in rsaA gene
false
Qimeng Gao
annotation2124041
1
BBa_K531001
range2124041
1
1006
1365
annotation2124040
1
BBa_K531003
range2124040
1
165
997
BBa_K525998_sequence
1
taatacgactcactatagggaaagaggagaaa
BBa_K2144002_sequence
1
taatacgactcactatagggaaagaggagaaatactagaggcggcttctagcatggaccgcccgcgcccgtgaggccgaggatttcgcgctggtcagacaacctacttgccgtccccacatgttagcgctaccaagtgccgacgaacgcgcgccgccgacggtgtcggcgcttcagacgctcgagttttggggagaaatactagatgaagaagcgcttcctgtcgatctgcaccatgaccatcgccgccctggccacgacgaccatggtgaacacgagctatgcgaagaccgacaccgagtcccacaaccactcgtcgctgggtaccgaaaacaagaatgtcctggacatcaacagcagctcccacaacatcaagccgagccagaacaagagctatccgagcgtgatcctgccgaataacaaccgccaccagatcttcaacacgacccaagggcactatgacgccgtgtcgttcatctacatcccgatcgacggcggctacatcccgatcgacggcggctacatgagcggctccggcgtcgtcgtcggcgaaacgagatcctcacgaataagcacgtcgtgaacggcgcgaagggcaacccccggaatatctccgtccatccctcggccaagaacgagaacgattatcccaatggtaagttcgtgggccaagaaatcatcccgtaccccggtaactcggacctggccatcctgcgcgtgagccccaacgagcacaatcaacacatcggccaggtggtcaagcccgcgacgatcagcagcaacacggacacccgcatcaacgagaacatcaccgtcaccggctaccccggcgacaagcccctggcgaccatgtgggagagcgtcggcaaggtggtctatatcggcggcgaggagctgcgctatgacctcagcacggtgggcggcaacagcggctcgcccgtcttcaacggcaagaaccaggtgatcggcatccactatggtggtgtcgacaacaagtataactcgagcgtctacatcaatgacttcgtgcagcaattcctgcgcaacaacatcccggacatcaacatccagactagagatatcaacgctatcggcacctcgaccgctttcgtgacgatcaccgacgccgctgtcggcgacaagctcgacctcgtcggcatctcgacgaacggcgctatcgctgacggcgccttcggcgctgcggtcaccctgggcgctgctgcgaccctggctcagtacctggacgctgctgctgccggcgacggcagcggcacctcggttgccaagtggttccagttcggcggcgacacctatgtcgtcgttgacagctcggctggcgcgaccttcgtcagcggcgctgacgcggtgatcaagctgaccggtctggtcacgctgaccacctcggccttcgccaccgaagtcctgacgctcgcctaa
BBa_K2144009_sequence
1
ggcggcggcggcagccttcctgaaactggcggccaccaccaccaccaccac
BBa_K531005_sequence
1
gcggcttctagcatggaccgcccgcgcccgtgaggccgaggatttcgcgctggtcagacaacctacttgccgtccccacatgttagcgctaccaagtgccgacgaacgcgcgccgccgacggtgtcggcgcttcagacgctcgagttttggggagaaatactagatgaagaagcgcttcctgtcgatctgcaccatgaccatcgccgccctggccacgacgaccatggtgaacacgagctatgcgaagaccgacaccgagtcccacaaccactcgtcgctgggtaccgaaaacaagaatgtcctggacatcaacagcagctcccacaacatcaagccgagccagaacaagagctatccgagcgtgatcctgccgaataacaaccgccaccagatcttcaacacgacccaagggcactatgacgccgtgtcgttcatctacatcccgatcgacggcggctacatcccgatcgacggcggctacatgagcggctccggcgtcgtcgtcggcgaaacgagatcctcacgaataagcacgtcgtgaacggcgcgaagggcaacccccggaatatctccgtccatccctcggccaagaacgagaacgattatcccaatggtaagttcgtgggccaagaaatcatcccgtaccccggtaactcggacctggccatcctgcgcgtgagccccaacgagcacaatcaacacatcggccaggtggtcaagcccgcgacgatcagcagcaacacggacacccgcatcaacgagaacatcaccgtcaccggctaccccggcgacaagcccctggcgaccatgtgggagagcgtcggcaaggtggtctatatcggcggcgaggagctgcgctatgacctcagcacggtgggcggcaacagcggctcgcccgtcttcaacggcaagaaccaggtgatcggcatccactatggtggtgtcgacaacaagtataactcgagcgtctacatcaatgacttcgtgcagcaattcctgcgcaacaacatcccggacatcaacatccagactagagatatcaacgctatcggcacctcgaccgctttcgtgacgatcaccgacgccgctgtcggcgacaagctcgacctcgtcggcatctcgacgaacggcgctatcgctgacggcgccttcggcgctgcggtcaccctgggcgctgctgcgaccctggctcagtacctggacgctgctgctgccggcgacggcagcggcacctcggttgccaagtggttccagttcggcggcgacacctatgtcgtcgttgacagctcggctggcgcgaccttcgtcagcggcgctgacgcggtgatcaagctgaccggtctggtcacgctgaccacctcggccttcgccaccgaagtcctgacgctcgcctaa
BBa_K2144006_sequence
1
taatacgactcactatagggaaagaggagaaatactagaggcggcttctagcatggaccgcccgcgcccgtgaggccgaggatttcgcgctggtcagacaacctacttgccgtccccacatgttagcgctaccaagtgccgacgaacgcgcgccgccgacggtgtcggcgcttcagacgctcgagttttggggagaaatactagatgaagaagcgcttcctgtcgatctgcaccatgaccatcgccgccctggccacgacgaccatggtgaacacgagctatgcgaagaccgacaccgagtcccacaaccactcgtcgctgggtaccgaaaacaagaatgtcctggacatcaacagcagctcccacaacatcaagccgagccagaacaagagctatccgagcgtgatcctgccgaataacaaccgccaccagatcttcaacacgacccaagggcactatgacgccgtgtcgttcatctacatcccgatcgacggcggctacatcccgatcgacggcggctacatgagcggctccggcgtcgtcgtcggcgaaacgagatcctcacgaataagcacgtcgtgaacggcgcgaagggcaacccccggaatatctccgtccatccctcggccaagaacgagaacgattatcccaatggtaagttcgtgggccaagaaatcatcccgtaccccggtaactcggacctggccatcctgcgcgtgagccccaacgagcacaatcaacacatcggccaggtggtcaagcccgcgacgatcagcagcaacacggacacccgcatcaacgagaacatcaccgtcaccggctaccccggcgacaagcccctggcgaccatgtgggagagcgtcggcaaggtggtctatatcggcggcgaggagctgcgctatgacctcagcacggtgggcggcaacagcggctcgcccgtcttcaacggcaagaaccaggtgatcggcatccactatggtggtgtcgacaacaagtataactcgagcgtctacatcaatgacttcgtgcagcaattcctgcgcaacaacatcccggacatcaacatccagactagagatatcaacgctatcggcacctcgaccgctttcgtgacgatcaccgacgccgctgtcggcgacaagctcgacctcgtcggcatctcgacgaacggcgctatcgctgacggcgccttcggcgctgcggtcaccctgggcgctgctgcgaccctggctcagtacctggacgctgctgctgccggcgacggcagcggcacctcggttgccaagtggttccagttcggcggcgacacctatgtcgtcgttgacagctcggctggcgcgaccttcgtcagcggcgctgacgcggtgatcaagctgaccggtctggtcacgctgaccacctcggccttcgccaccgaagtcctgacgctcgcctaatactagagggcggcggcggcagccttcctgaaactggcggccaccaccaccaccaccac
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z