BBa_K2144006 1 BBa_K2144006 Coding sequence for ESP with His6 and LPXTG-tag regulated by T7-promoter 2016-10-11T11:00:00Z 2016-10-12T03:20:01Z - Esp, extracellular serine protease, is capable of inhibit the biofilm formation and nasal colonization of S. aureus. This by protelysing receptors essential for biofilm formation and prevention of eDNA exudation. The inserted LT-sequence contain the gene encoding for His-tag which enables the use of IMAC to purify the combat protein. Furthermore it contains the LXPTG-tag which enables the use of the "match making" enzyme Sortase. The key feature of sortase is the specific conjugation reaction it carries out, where the enzyme recognizes a specific amino acid sequence, a so called sorting motif (LPXTG motif in the case of S.aureus) and conjugate this sequence with another unit carrying an oligo glycine motif. A new peptide bond is formed. The Linker in the LT-sequence enables proper folding of the protein. false false _2614_ 31517 31517 9 false The LT-sequence was inserted with Overhang PCR. false Oskar ??hman component2493561 1 BBa_K2144009 component2493557 1 BBa_K2144002 annotation2493561 1 BBa_K2144009 range2493561 1 1445 1495 annotation2493557 1 BBa_K2144002 range2493557 1 1 1436 BBa_K2144009 1 BBa_K2144009 Sequence for His6-tag and LPXTGG-tag 2016-10-11T11:00:00Z 2016-10-12T02:35:45Z Coming soon The His6-tag enables purification of proteins with IMAC. The LPXTG-tag enables the use of the "match making" enzyme sortase. The key feature of sortasee is the specific conjugation reaction it carries out, where the enzyme recognizes a specific amino acid sequence, a so called sorting motif (LPXTG motif in the case of S.aureus) and conjugate this sequence with another unit carrying an oligo glycine motif. false false _2614_ 31517 31517 9 false Coming soon false Oskar ??hman annotation2493493 1 Linker range2493493 1 1 14 annotation2493495 1 His6 range2493495 1 32 48 annotation2493494 1 LPXTG range2493494 1 15 31 BBa_K525998 1 pT7 Promoter T7 and RBS 2011-09-12T11:00:00Z 2015-05-08T01:12:35Z Synthesis Released HQ 2013 Promoter T7 and RBS false false _690_ 0 8543 9 In stock false Synthesis false Anna Drong annotation2129433 1 RBS range2129433 1 24 27 annotation2129432 1 T7 promoter range2129432 1 1 20 annotation2127788 1 B0034 range2127788 1 21 32 BBa_K2144002 1 BBa_K2144002 ESP capable of inhibiting biofilm 2016-10-11T11:00:00Z 2016-10-12T01:57:08Z Coming soon. Esp, extracellular serine protease, is capable of inhibit the biofilm formation and nasal colonization of S. aureus. This by protelysing receptors essential for biofilm formation and prevention of eDNA exudation. false false _2614_ 31517 31517 9 false Basic 3A Assembly was performed to make this BioBrick false Oskar ??hman component2493468 1 BBa_K531005 component2493465 1 BBa_K525998 annotation2493465 1 BBa_K525998 range2493465 1 1 32 annotation2493468 1 BBa_K531005 range2493468 1 41 1436 BBa_K531005 1 BBa_K531005 P_xyl promoter+optimized <i>esp</i>+<i>rsaA</i> secretion tag 2011-07-25T11:00:00Z 2015-05-08T01:12:36Z From BBa_K531001 BBa_K531002 BBa_K531003 The BioBrick part enables C. crescentus to express and secret Esp protein in the presence of xylose. false false _697_ 0 8860 9 In stock false no stop codon in esp gene and no start codon in rsaA gene false Qimeng Gao annotation2124041 1 BBa_K531001 range2124041 1 1006 1365 annotation2124040 1 BBa_K531003 range2124040 1 165 997 BBa_K525998_sequence 1 taatacgactcactatagggaaagaggagaaa BBa_K2144002_sequence 1 taatacgactcactatagggaaagaggagaaatactagaggcggcttctagcatggaccgcccgcgcccgtgaggccgaggatttcgcgctggtcagacaacctacttgccgtccccacatgttagcgctaccaagtgccgacgaacgcgcgccgccgacggtgtcggcgcttcagacgctcgagttttggggagaaatactagatgaagaagcgcttcctgtcgatctgcaccatgaccatcgccgccctggccacgacgaccatggtgaacacgagctatgcgaagaccgacaccgagtcccacaaccactcgtcgctgggtaccgaaaacaagaatgtcctggacatcaacagcagctcccacaacatcaagccgagccagaacaagagctatccgagcgtgatcctgccgaataacaaccgccaccagatcttcaacacgacccaagggcactatgacgccgtgtcgttcatctacatcccgatcgacggcggctacatcccgatcgacggcggctacatgagcggctccggcgtcgtcgtcggcgaaacgagatcctcacgaataagcacgtcgtgaacggcgcgaagggcaacccccggaatatctccgtccatccctcggccaagaacgagaacgattatcccaatggtaagttcgtgggccaagaaatcatcccgtaccccggtaactcggacctggccatcctgcgcgtgagccccaacgagcacaatcaacacatcggccaggtggtcaagcccgcgacgatcagcagcaacacggacacccgcatcaacgagaacatcaccgtcaccggctaccccggcgacaagcccctggcgaccatgtgggagagcgtcggcaaggtggtctatatcggcggcgaggagctgcgctatgacctcagcacggtgggcggcaacagcggctcgcccgtcttcaacggcaagaaccaggtgatcggcatccactatggtggtgtcgacaacaagtataactcgagcgtctacatcaatgacttcgtgcagcaattcctgcgcaacaacatcccggacatcaacatccagactagagatatcaacgctatcggcacctcgaccgctttcgtgacgatcaccgacgccgctgtcggcgacaagctcgacctcgtcggcatctcgacgaacggcgctatcgctgacggcgccttcggcgctgcggtcaccctgggcgctgctgcgaccctggctcagtacctggacgctgctgctgccggcgacggcagcggcacctcggttgccaagtggttccagttcggcggcgacacctatgtcgtcgttgacagctcggctggcgcgaccttcgtcagcggcgctgacgcggtgatcaagctgaccggtctggtcacgctgaccacctcggccttcgccaccgaagtcctgacgctcgcctaa BBa_K2144009_sequence 1 ggcggcggcggcagccttcctgaaactggcggccaccaccaccaccaccac BBa_K531005_sequence 1 gcggcttctagcatggaccgcccgcgcccgtgaggccgaggatttcgcgctggtcagacaacctacttgccgtccccacatgttagcgctaccaagtgccgacgaacgcgcgccgccgacggtgtcggcgcttcagacgctcgagttttggggagaaatactagatgaagaagcgcttcctgtcgatctgcaccatgaccatcgccgccctggccacgacgaccatggtgaacacgagctatgcgaagaccgacaccgagtcccacaaccactcgtcgctgggtaccgaaaacaagaatgtcctggacatcaacagcagctcccacaacatcaagccgagccagaacaagagctatccgagcgtgatcctgccgaataacaaccgccaccagatcttcaacacgacccaagggcactatgacgccgtgtcgttcatctacatcccgatcgacggcggctacatcccgatcgacggcggctacatgagcggctccggcgtcgtcgtcggcgaaacgagatcctcacgaataagcacgtcgtgaacggcgcgaagggcaacccccggaatatctccgtccatccctcggccaagaacgagaacgattatcccaatggtaagttcgtgggccaagaaatcatcccgtaccccggtaactcggacctggccatcctgcgcgtgagccccaacgagcacaatcaacacatcggccaggtggtcaagcccgcgacgatcagcagcaacacggacacccgcatcaacgagaacatcaccgtcaccggctaccccggcgacaagcccctggcgaccatgtgggagagcgtcggcaaggtggtctatatcggcggcgaggagctgcgctatgacctcagcacggtgggcggcaacagcggctcgcccgtcttcaacggcaagaaccaggtgatcggcatccactatggtggtgtcgacaacaagtataactcgagcgtctacatcaatgacttcgtgcagcaattcctgcgcaacaacatcccggacatcaacatccagactagagatatcaacgctatcggcacctcgaccgctttcgtgacgatcaccgacgccgctgtcggcgacaagctcgacctcgtcggcatctcgacgaacggcgctatcgctgacggcgccttcggcgctgcggtcaccctgggcgctgctgcgaccctggctcagtacctggacgctgctgctgccggcgacggcagcggcacctcggttgccaagtggttccagttcggcggcgacacctatgtcgtcgttgacagctcggctggcgcgaccttcgtcagcggcgctgacgcggtgatcaagctgaccggtctggtcacgctgaccacctcggccttcgccaccgaagtcctgacgctcgcctaa BBa_K2144006_sequence 1 taatacgactcactatagggaaagaggagaaatactagaggcggcttctagcatggaccgcccgcgcccgtgaggccgaggatttcgcgctggtcagacaacctacttgccgtccccacatgttagcgctaccaagtgccgacgaacgcgcgccgccgacggtgtcggcgcttcagacgctcgagttttggggagaaatactagatgaagaagcgcttcctgtcgatctgcaccatgaccatcgccgccctggccacgacgaccatggtgaacacgagctatgcgaagaccgacaccgagtcccacaaccactcgtcgctgggtaccgaaaacaagaatgtcctggacatcaacagcagctcccacaacatcaagccgagccagaacaagagctatccgagcgtgatcctgccgaataacaaccgccaccagatcttcaacacgacccaagggcactatgacgccgtgtcgttcatctacatcccgatcgacggcggctacatcccgatcgacggcggctacatgagcggctccggcgtcgtcgtcggcgaaacgagatcctcacgaataagcacgtcgtgaacggcgcgaagggcaacccccggaatatctccgtccatccctcggccaagaacgagaacgattatcccaatggtaagttcgtgggccaagaaatcatcccgtaccccggtaactcggacctggccatcctgcgcgtgagccccaacgagcacaatcaacacatcggccaggtggtcaagcccgcgacgatcagcagcaacacggacacccgcatcaacgagaacatcaccgtcaccggctaccccggcgacaagcccctggcgaccatgtgggagagcgtcggcaaggtggtctatatcggcggcgaggagctgcgctatgacctcagcacggtgggcggcaacagcggctcgcccgtcttcaacggcaagaaccaggtgatcggcatccactatggtggtgtcgacaacaagtataactcgagcgtctacatcaatgacttcgtgcagcaattcctgcgcaacaacatcccggacatcaacatccagactagagatatcaacgctatcggcacctcgaccgctttcgtgacgatcaccgacgccgctgtcggcgacaagctcgacctcgtcggcatctcgacgaacggcgctatcgctgacggcgccttcggcgctgcggtcaccctgggcgctgctgcgaccctggctcagtacctggacgctgctgctgccggcgacggcagcggcacctcggttgccaagtggttccagttcggcggcgacacctatgtcgtcgttgacagctcggctggcgcgaccttcgtcagcggcgctgacgcggtgatcaagctgaccggtctggtcacgctgaccacctcggccttcgccaccgaagtcctgacgctcgcctaatactagagggcggcggcggcagccttcctgaaactggcggccaccaccaccaccaccac igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z