LOCUS       BBa_K2144007             259 bp    DNA     linear       22-Dec-2024
DEFINITION  Coding sequence for Defensin with His6 and LPXTG-tag regulated by 
            T7-promoter
ACCESSION   BBa_K2144007
VERSION     BBa_K2144007.1  
FEATURES             Location/Qualifiers
     misc_feature    21..32
                     /label=B0034
     promoter        1..20
                     /label=T7 promoter
     RBS             24..27
                     /label=RBS
     promoter        1..32
                     /label=BBa_K525998
     misc_feature    39..41
                     /label=Methionine
     misc_feature    198..200
                     /label=Term
     CDS             42..197
                     /label=mature peptide
     CDS             39..200
                     /label=BBa_K1104301
     CDS             1..200
                     /label=BBa_K2144003
     misc_feature    209..222
                     /label=Linker
     misc_feature    223..239
                     /label=LPXTG
     misc_feature    240..256
                     /label=His6
     misc_feature    209..259
                     /label=BBa_K2144009
ORIGIN
        1 taatacgact cactataggg aaagaggaga aatactagat ggtaacttgt gaccttctct
       61 cattcaaagg acaagttaat gacagtgctt gcgctgctaa ctgtctcagt ttgggtaaag
      121 ctggaggtca ttgcgagaaa ggagtttgta tttgtcgaaa aaccagtttc aaagatctct
      181 gggacaaacg tttcggttaa tactagaggg cggcggcggc agccttcctg aaactggcgg
      241 ccaccaccac caccaccac
//