BBa_K2144008 1 BBa_K2144008 Coding sequence for Sortase A with His-tag 2016-10-12T11:00:00Z 2016-10-13T01:11:52Z iGEM 2016 isolated this part from S.Aureus Sortase A is a bacterial enzyme with the ability to break and form new peptide bonds. The key feature of the enzyme is the specific conjugation reaction it carries out, where the enzyme recognizes a specific amino acid sequence, a so called sorting motif (LPXTG motif in the case of S.aureus) and conjugate this sequence with another unit carrying an oligo glycine motif. A new peptide bond is formed. This Biobrick also contains GB1 and a His-tag to enable IMAC-purification. false false _2614_ 31517 31517 9 false - false Oskar ??hman annotation2496409 1 His-tag range2496409 1 610 625 annotation2496408 1 Sortase A range2496408 1 165 609 annotation2496406 1 GB1 range2496406 1 1 164 BBa_K2144008_sequence 1 atgacatataaattaattttaaatggtaaaacattaaaaggtgaaacaacaacagaagctgttgatgctgctacagctgaaaaagtttttaaacaatatgctaatgataatggtgttgatggtgaatggacatatgatgatgctacaaaaacatttacagttacagaacaagctaaacctcaaattccgaaagataaatcgaaagtggcaggctatattgaaattccagatgctgatattaaagaaccagtatatccaggaccagcaacacctgaacaattaaatagaggtgtaagctttgcagaagaaaatgaatcactagatgatcaaaatatttcaattgcaggacacactttcattgaccgtccgaactatcaatttacaaatcttaaagcagccaaaaaaggtagtatggtgtactttaaagttggtaatgaaacacgtaagtataaaatgacaagtataagagatgttaagcctacagatgtaggagttctcgatgaacaaaaaggtaaagataaacaattaacattaattacttgtgatgattacaatgaaaagacaggcgtttgggaaaaacgtaaaatctttgtagctacagaagtcaaacatcatcaccatcaccactaaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z