BBa_K2144009
1
BBa_K2144009
Sequence for His6-tag and LPXTGG-tag
2016-10-11T11:00:00Z
2016-10-12T02:35:45Z
Coming soon
The His6-tag enables purification of proteins with IMAC. The LPXTG-tag enables the use of the "match making" enzyme sortase. The key feature of sortasee is the specific conjugation reaction it carries out, where the enzyme recognizes a specific amino acid sequence, a so called sorting motif (LPXTG motif in the case of S.aureus) and conjugate this sequence with another unit carrying an oligo glycine motif.
false
false
_2614_
31517
31517
9
false
Coming soon
false
Oskar ??hman
annotation2493493
1
Linker
range2493493
1
1
14
annotation2493494
1
LPXTG
range2493494
1
15
31
annotation2493495
1
His6
range2493495
1
32
48
BBa_K2144009_sequence
1
ggcggcggcggcagccttcctgaaactggcggccaccaccaccaccaccac
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z