LOCUS       BBa_K2144011             658 bp    DNA     linear       22-Nov-2024
DEFINITION  Coding sequence for Nuclease with His6 and LPXTG tag regulated by 
            T7-promoter
ACCESSION   BBa_K2144011
VERSION     BBa_K2144011.1  
FEATURES             Location/Qualifiers
     misc_feature    21..32
                     /label=B0034
     promoter        1..20
                     /label=T7 promoter
     RBS             24..27
                     /label=RBS
     promoter        1..32
                     /label=BBa_K525998
     CDS             39..599
                     /label=BBa_K729004
     CDS             1..599
                     /label=BBa_K2144000
     misc_feature    608..621
                     /label=Linker
     misc_feature    622..638
                     /label=LPXTG
     misc_feature    639..655
                     /label=His6
     misc_feature    608..658
                     /label=BBa_K2144009
ORIGIN
        1 taatacgact cactataggg aaagaggaga aatactagat gaaaaagatt tggctggcgc
       61 tggctggttt agttttagcg tttagcgcat cggcgcaaac agataacggc gtaaatagaa
      121 gtggttctga agatccaaca gtatatagtg caacttcaac taaaaaatta cataaagaac
      181 ctgcgacttt aattaaagcg attgatggtg atacggttaa attaatgtac aaaggtcaac
      241 caatgacatt cagactatta ttggttgata cacctgaaac aaagcatcct aaaaaaggtg
      301 tagagaaata tggtcctgaa gcaagtgcat ttacgaaaaa aatggtagaa aatgcaaaga
      361 aaattgaagt cgagtttgac aaaggtcaaa gaactgataa atatggacgt ggcttagcgt
      421 atatttatgc tgatggaaaa atggtaaacg aagctttagt tcgtcaaggc ttggctaaag
      481 ttgcttatgt ttacaaacct aacaatacac atgaacaaca tttaagaaaa agtgaagcac
      541 aagcgaaaaa agagaaatta aatatttgga gcgaagacaa cgctgattca ggtcaataat
      601 actagagggc ggcggcggca gccttcctga aactggcggc caccaccacc accaccac
//