LOCUS BBa_K2144012 932 bp DNA linear 31-Oct-2024 DEFINITION Coding sequence for ESP with His6 and LPXTG-tag regulated by T7-promoter ACCESSION BBa_K2144012 VERSION BBa_K2144012.1 FEATURES Location/Qualifiers misc_feature 21..32 /label=B0034 promoter 1..20 /label=T7 promoter RBS 24..27 /label=RBS promoter 1..32 /label=BBa_K525998 CDS 41..873 /label=BBa_K531003 CDS 1..873 /label=BBa_K2144010 misc_feature 882..895 /label=Linker misc_feature 896..912 /label=LPXTG misc_feature 913..929 /label=His6 misc_feature 882..932 /label=BBa_K2144009 ORIGIN 1 taatacgact cactataggg aaagaggaga aatactagag gtctgaatat ttatatcagg 61 tatattgttt cttaggaatt gttgaacgaa atcattaata taaacactgc tattgtattt 121 attatctacg ccaccataat gtattccaat aacttgattt ttaccattaa atactggaga 181 tccagagttt ccacctacag tacttaggtc atatcttaat tcctcgccac caatatagac 241 aactttacct acactttccc acattgtggc taatggtttg tcaccagggt aaccagtaac 301 agtgatgttt tcattaattc tagtgtctgt attgctactt atagttgcag gtttaactac 361 ttgaccaata tgttgattat gttcgtttgg tgacactctt aagattgcta aatcactatt 421 accaggatac ggtatgattt cttgacccac aaatttgcca ttaggataat cattttcatt 481 tttagctgaa ggatggacac taatatttct tggattaccc ttagctccat taacaacgtg 541 tttattagtt aatatttcat tttcacctac aacaacacct gaaccactca tatatccacc 601 atctattggt atataaataa aactaacagc atcataatga ccttgtgtag tattaaaaat 661 ttgatgtcta ttattattag gtaatattac acttgggtaa cttttatttt gacttggttt 721 gatattatga ctcgaactat taatatctaa aacatttttg ttttctgtgc caagtgagga 781 atgattatgg ctttctgtat cggtttttgc ataagaagta tttaccattg tagtagttgc 841 taacgctgca attgtcattg tacatataga cattactaga gggcggcggc ggcagccttc 901 ctgaaactgg cggccaccac caccaccacc ac //