LOCUS       BBa_K2144012             932 bp    DNA     linear       12-Jun-2024
DEFINITION  Coding sequence for ESP with His6 and LPXTG-tag regulated by 
            T7-promoter
ACCESSION   BBa_K2144012
VERSION     BBa_K2144012.1  
FEATURES             Location/Qualifiers
     misc_feature    21..32
                     /label=B0034
     promoter        1..20
                     /label=T7 promoter
     RBS             24..27
                     /label=RBS
     promoter        1..32
                     /label=BBa_K525998
     CDS             41..873
                     /label=BBa_K531003
     CDS             1..873
                     /label=BBa_K2144010
     misc_feature    882..895
                     /label=Linker
     misc_feature    896..912
                     /label=LPXTG
     misc_feature    913..929
                     /label=His6
     misc_feature    882..932
                     /label=BBa_K2144009
ORIGIN
        1 taatacgact cactataggg aaagaggaga aatactagag gtctgaatat ttatatcagg
       61 tatattgttt cttaggaatt gttgaacgaa atcattaata taaacactgc tattgtattt
      121 attatctacg ccaccataat gtattccaat aacttgattt ttaccattaa atactggaga
      181 tccagagttt ccacctacag tacttaggtc atatcttaat tcctcgccac caatatagac
      241 aactttacct acactttccc acattgtggc taatggtttg tcaccagggt aaccagtaac
      301 agtgatgttt tcattaattc tagtgtctgt attgctactt atagttgcag gtttaactac
      361 ttgaccaata tgttgattat gttcgtttgg tgacactctt aagattgcta aatcactatt
      421 accaggatac ggtatgattt cttgacccac aaatttgcca ttaggataat cattttcatt
      481 tttagctgaa ggatggacac taatatttct tggattaccc ttagctccat taacaacgtg
      541 tttattagtt aatatttcat tttcacctac aacaacacct gaaccactca tatatccacc
      601 atctattggt atataaataa aactaacagc atcataatga ccttgtgtag tattaaaaat
      661 ttgatgtcta ttattattag gtaatattac acttgggtaa cttttatttt gacttggttt
      721 gatattatga ctcgaactat taatatctaa aacatttttg ttttctgtgc caagtgagga
      781 atgattatgg ctttctgtat cggtttttgc ataagaagta tttaccattg tagtagttgc
      841 taacgctgca attgtcattg tacatataga cattactaga gggcggcggc ggcagccttc
      901 ctgaaactgg cggccaccac caccaccacc ac
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