BBa_K2144012
1
BBa_K2144012
Coding sequence for ESP with His6 and LPXTG-tag regulated by T7-promoter
2016-10-12T11:00:00Z
2016-10-13T10:13:53Z
-
Esp, extracellular serine protease, is capable of inhibit the biofilm formation and nasal colonization of S. aureus. This by protelysing receptors essential for biofilm formation and prevention of eDNA exudation.
The inserted LT-sequence contain the gene encoding for His-tag which enables the use of IMAC to purify the combat protein. Furthermore it contains the LXPTG-tag which enables the use of the "match making" enzyme Sortase. The key feature of sortase is the specific conjugation reaction it carries out, where the enzyme recognizes a specific amino acid sequence, a so called sorting motif (LPXTG motif in the case of S.aureus) and conjugate this sequence with another unit carrying an oligo glycine motif. A new peptide bond is formed. The Linker in the LT-sequence enables proper folding of the protein.
false
false
_2614_
31517
31517
9
false
Overhang PCR was performed to insert the Linker-tag-sequence.
false
Oskar ??hman
component2498924
1
BBa_K2144010
component2498928
1
BBa_K2144009
annotation2498924
1
BBa_K2144010
range2498924
1
1
873
annotation2498928
1
BBa_K2144009
range2498928
1
882
932
BBa_K531003
1
BBa_K531003
Esp coding sequence from Staphylococcus epidermidis
2011-07-04T11:00:00Z
2015-05-08T01:12:36Z
Zhang,Y.Q., Ren,S.X., Li,H.L., Wang,Y.X., Fu,G., Yang,J., Qin,Z.Q., Miao,Y.G., Wang,W.Y., Chen,R.S., Shen,Y., Chen,Z., Yuan,Z.H., Zhao,G.P., Qu,D., Danchin,A. and Wen,Y.M. Genome-based analysis of virulence genes in a non-biofilm-forming Staphylococcus epidermidis strain (ATCC 12228). Mol. Microbiol. 49 (6), 1577-1593 (2003).
Staphylococcus epidermidis, a commensal bacterium, was observed to secret the serine protease Esp to inhibit biofilm formation and nasal colonization by Staphylococcus aureus, a human pathogen.
false
false
_697_
0
8860
9
It's complicated
false
eliminate stop codon
false
Qimeng Gao
BBa_K2144010
1
BBa_K2144010
Coding sequence for Esp regulated by T7-promoter
2016-10-12T11:00:00Z
2016-10-19T03:01:31Z
-
Esp, extracellular serine protease, is capable of inhibit the biofilm formation and nasal colonization of S. aureus. This by protelysing receptors essential for biofilm formation and prevention of eDNA exudation.
false
false
_2614_
31509
31517
9
false
This construct was created by 3A assembly
false
Oskar ??hman
component2498906
1
BBa_K531003
component2498905
1
BBa_K525998
annotation2498906
1
BBa_K531003
range2498906
1
41
873
annotation2498905
1
BBa_K525998
range2498905
1
1
32
BBa_K2144009
1
BBa_K2144009
Sequence for His6-tag and LPXTGG-tag
2016-10-11T11:00:00Z
2016-10-12T02:35:45Z
Coming soon
The His6-tag enables purification of proteins with IMAC. The LPXTG-tag enables the use of the "match making" enzyme sortase. The key feature of sortasee is the specific conjugation reaction it carries out, where the enzyme recognizes a specific amino acid sequence, a so called sorting motif (LPXTG motif in the case of S.aureus) and conjugate this sequence with another unit carrying an oligo glycine motif.
false
false
_2614_
31517
31517
9
false
Coming soon
false
Oskar ??hman
annotation2493495
1
His6
range2493495
1
32
48
annotation2493493
1
Linker
range2493493
1
1
14
annotation2493494
1
LPXTG
range2493494
1
15
31
BBa_K525998
1
pT7
Promoter T7 and RBS
2011-09-12T11:00:00Z
2015-05-08T01:12:35Z
Synthesis
Released HQ 2013
Promoter T7 and RBS
false
false
_690_
0
8543
9
In stock
false
Synthesis
false
Anna Drong
annotation2129433
1
RBS
range2129433
1
24
27
annotation2129432
1
T7 promoter
range2129432
1
1
20
annotation2127788
1
B0034
range2127788
1
21
32
BBa_K531003_sequence
1
gtctgaatatttatatcaggtatattgtttcttaggaattgttgaacgaaatcattaatataaacactgctattgtatttattatctacgccaccataatgtattccaataacttgatttttaccattaaatactggagatccagagtttccacctacagtacttaggtcatatcttaattcctcgccaccaatatagacaactttacctacactttcccacattgtggctaatggtttgtcaccagggtaaccagtaacagtgatgttttcattaattctagtgtctgtattgctacttatagttgcaggtttaactacttgaccaatatgttgattatgttcgtttggtgacactcttaagattgctaaatcactattaccaggatacggtatgatttcttgacccacaaatttgccattaggataatcattttcatttttagctgaaggatggacactaatatttcttggattacccttagctccattaacaacgtgtttattagttaatatttcattttcacctacaacaacacctgaaccactcatatatccaccatctattggtatataaataaaactaacagcatcataatgaccttgtgtagtattaaaaatttgatgtctattattattaggtaatattacacttgggtaacttttattttgacttggtttgatattatgactcgaactattaatatctaaaacatttttgttttctgtgccaagtgaggaatgattatggctttctgtatcggtttttgcataagaagtatttaccattgtagtagttgctaacgctgcaattgtcattgtacatatagacat
BBa_K2144012_sequence
1
taatacgactcactatagggaaagaggagaaatactagaggtctgaatatttatatcaggtatattgtttcttaggaattgttgaacgaaatcattaatataaacactgctattgtatttattatctacgccaccataatgtattccaataacttgatttttaccattaaatactggagatccagagtttccacctacagtacttaggtcatatcttaattcctcgccaccaatatagacaactttacctacactttcccacattgtggctaatggtttgtcaccagggtaaccagtaacagtgatgttttcattaattctagtgtctgtattgctacttatagttgcaggtttaactacttgaccaatatgttgattatgttcgtttggtgacactcttaagattgctaaatcactattaccaggatacggtatgatttcttgacccacaaatttgccattaggataatcattttcatttttagctgaaggatggacactaatatttcttggattacccttagctccattaacaacgtgtttattagttaatatttcattttcacctacaacaacacctgaaccactcatatatccaccatctattggtatataaataaaactaacagcatcataatgaccttgtgtagtattaaaaatttgatgtctattattattaggtaatattacacttgggtaacttttattttgacttggtttgatattatgactcgaactattaatatctaaaacatttttgttttctgtgccaagtgaggaatgattatggctttctgtatcggtttttgcataagaagtatttaccattgtagtagttgctaacgctgcaattgtcattgtacatatagacattactagagggcggcggcggcagccttcctgaaactggcggccaccaccaccaccaccac
BBa_K2144010_sequence
1
taatacgactcactatagggaaagaggagaaatactagaggtctgaatatttatatcaggtatattgtttcttaggaattgttgaacgaaatcattaatataaacactgctattgtatttattatctacgccaccataatgtattccaataacttgatttttaccattaaatactggagatccagagtttccacctacagtacttaggtcatatcttaattcctcgccaccaatatagacaactttacctacactttcccacattgtggctaatggtttgtcaccagggtaaccagtaacagtgatgttttcattaattctagtgtctgtattgctacttatagttgcaggtttaactacttgaccaatatgttgattatgttcgtttggtgacactcttaagattgctaaatcactattaccaggatacggtatgatttcttgacccacaaatttgccattaggataatcattttcatttttagctgaaggatggacactaatatttcttggattacccttagctccattaacaacgtgtttattagttaatatttcattttcacctacaacaacacctgaaccactcatatatccaccatctattggtatataaataaaactaacagcatcataatgaccttgtgtagtattaaaaatttgatgtctattattattaggtaatattacacttgggtaacttttattttgacttggtttgatattatgactcgaactattaatatctaaaacatttttgttttctgtgccaagtgaggaatgattatggctttctgtatcggtttttgcataagaagtatttaccattgtagtagttgctaacgctgcaattgtcattgtacatatagacat
BBa_K525998_sequence
1
taatacgactcactatagggaaagaggagaaa
BBa_K2144009_sequence
1
ggcggcggcggcagccttcctgaaactggcggccaccaccaccaccaccac
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z