BBa_K2144012 1 BBa_K2144012 Coding sequence for ESP with His6 and LPXTG-tag regulated by T7-promoter 2016-10-12T11:00:00Z 2016-10-13T10:13:53Z - Esp, extracellular serine protease, is capable of inhibit the biofilm formation and nasal colonization of S. aureus. This by protelysing receptors essential for biofilm formation and prevention of eDNA exudation. The inserted LT-sequence contain the gene encoding for His-tag which enables the use of IMAC to purify the combat protein. Furthermore it contains the LXPTG-tag which enables the use of the "match making" enzyme Sortase. The key feature of sortase is the specific conjugation reaction it carries out, where the enzyme recognizes a specific amino acid sequence, a so called sorting motif (LPXTG motif in the case of S.aureus) and conjugate this sequence with another unit carrying an oligo glycine motif. A new peptide bond is formed. The Linker in the LT-sequence enables proper folding of the protein. false false _2614_ 31517 31517 9 false Overhang PCR was performed to insert the Linker-tag-sequence. false Oskar ??hman component2498924 1 BBa_K2144010 component2498928 1 BBa_K2144009 annotation2498924 1 BBa_K2144010 range2498924 1 1 873 annotation2498928 1 BBa_K2144009 range2498928 1 882 932 BBa_K531003 1 BBa_K531003 Esp coding sequence from Staphylococcus epidermidis 2011-07-04T11:00:00Z 2015-05-08T01:12:36Z Zhang,Y.Q., Ren,S.X., Li,H.L., Wang,Y.X., Fu,G., Yang,J., Qin,Z.Q., Miao,Y.G., Wang,W.Y., Chen,R.S., Shen,Y., Chen,Z., Yuan,Z.H., Zhao,G.P., Qu,D., Danchin,A. and Wen,Y.M. Genome-based analysis of virulence genes in a non-biofilm-forming Staphylococcus epidermidis strain (ATCC 12228). Mol. Microbiol. 49 (6), 1577-1593 (2003). Staphylococcus epidermidis, a commensal bacterium, was observed to secret the serine protease Esp to inhibit biofilm formation and nasal colonization by Staphylococcus aureus, a human pathogen. false false _697_ 0 8860 9 It's complicated false eliminate stop codon false Qimeng Gao BBa_K2144010 1 BBa_K2144010 Coding sequence for Esp regulated by T7-promoter 2016-10-12T11:00:00Z 2016-10-19T03:01:31Z - Esp, extracellular serine protease, is capable of inhibit the biofilm formation and nasal colonization of S. aureus. This by protelysing receptors essential for biofilm formation and prevention of eDNA exudation. false false _2614_ 31509 31517 9 false This construct was created by 3A assembly false Oskar ??hman component2498906 1 BBa_K531003 component2498905 1 BBa_K525998 annotation2498906 1 BBa_K531003 range2498906 1 41 873 annotation2498905 1 BBa_K525998 range2498905 1 1 32 BBa_K2144009 1 BBa_K2144009 Sequence for His6-tag and LPXTGG-tag 2016-10-11T11:00:00Z 2016-10-12T02:35:45Z Coming soon The His6-tag enables purification of proteins with IMAC. The LPXTG-tag enables the use of the "match making" enzyme sortase. The key feature of sortasee is the specific conjugation reaction it carries out, where the enzyme recognizes a specific amino acid sequence, a so called sorting motif (LPXTG motif in the case of S.aureus) and conjugate this sequence with another unit carrying an oligo glycine motif. false false _2614_ 31517 31517 9 false Coming soon false Oskar ??hman annotation2493495 1 His6 range2493495 1 32 48 annotation2493493 1 Linker range2493493 1 1 14 annotation2493494 1 LPXTG range2493494 1 15 31 BBa_K525998 1 pT7 Promoter T7 and RBS 2011-09-12T11:00:00Z 2015-05-08T01:12:35Z Synthesis Released HQ 2013 Promoter T7 and RBS false false _690_ 0 8543 9 In stock false Synthesis false Anna Drong annotation2129433 1 RBS range2129433 1 24 27 annotation2129432 1 T7 promoter range2129432 1 1 20 annotation2127788 1 B0034 range2127788 1 21 32 BBa_K531003_sequence 1 gtctgaatatttatatcaggtatattgtttcttaggaattgttgaacgaaatcattaatataaacactgctattgtatttattatctacgccaccataatgtattccaataacttgatttttaccattaaatactggagatccagagtttccacctacagtacttaggtcatatcttaattcctcgccaccaatatagacaactttacctacactttcccacattgtggctaatggtttgtcaccagggtaaccagtaacagtgatgttttcattaattctagtgtctgtattgctacttatagttgcaggtttaactacttgaccaatatgttgattatgttcgtttggtgacactcttaagattgctaaatcactattaccaggatacggtatgatttcttgacccacaaatttgccattaggataatcattttcatttttagctgaaggatggacactaatatttcttggattacccttagctccattaacaacgtgtttattagttaatatttcattttcacctacaacaacacctgaaccactcatatatccaccatctattggtatataaataaaactaacagcatcataatgaccttgtgtagtattaaaaatttgatgtctattattattaggtaatattacacttgggtaacttttattttgacttggtttgatattatgactcgaactattaatatctaaaacatttttgttttctgtgccaagtgaggaatgattatggctttctgtatcggtttttgcataagaagtatttaccattgtagtagttgctaacgctgcaattgtcattgtacatatagacat BBa_K2144012_sequence 1 taatacgactcactatagggaaagaggagaaatactagaggtctgaatatttatatcaggtatattgtttcttaggaattgttgaacgaaatcattaatataaacactgctattgtatttattatctacgccaccataatgtattccaataacttgatttttaccattaaatactggagatccagagtttccacctacagtacttaggtcatatcttaattcctcgccaccaatatagacaactttacctacactttcccacattgtggctaatggtttgtcaccagggtaaccagtaacagtgatgttttcattaattctagtgtctgtattgctacttatagttgcaggtttaactacttgaccaatatgttgattatgttcgtttggtgacactcttaagattgctaaatcactattaccaggatacggtatgatttcttgacccacaaatttgccattaggataatcattttcatttttagctgaaggatggacactaatatttcttggattacccttagctccattaacaacgtgtttattagttaatatttcattttcacctacaacaacacctgaaccactcatatatccaccatctattggtatataaataaaactaacagcatcataatgaccttgtgtagtattaaaaatttgatgtctattattattaggtaatattacacttgggtaacttttattttgacttggtttgatattatgactcgaactattaatatctaaaacatttttgttttctgtgccaagtgaggaatgattatggctttctgtatcggtttttgcataagaagtatttaccattgtagtagttgctaacgctgcaattgtcattgtacatatagacattactagagggcggcggcggcagccttcctgaaactggcggccaccaccaccaccaccac BBa_K2144010_sequence 1 taatacgactcactatagggaaagaggagaaatactagaggtctgaatatttatatcaggtatattgtttcttaggaattgttgaacgaaatcattaatataaacactgctattgtatttattatctacgccaccataatgtattccaataacttgatttttaccattaaatactggagatccagagtttccacctacagtacttaggtcatatcttaattcctcgccaccaatatagacaactttacctacactttcccacattgtggctaatggtttgtcaccagggtaaccagtaacagtgatgttttcattaattctagtgtctgtattgctacttatagttgcaggtttaactacttgaccaatatgttgattatgttcgtttggtgacactcttaagattgctaaatcactattaccaggatacggtatgatttcttgacccacaaatttgccattaggataatcattttcatttttagctgaaggatggacactaatatttcttggattacccttagctccattaacaacgtgtttattagttaatatttcattttcacctacaacaacacctgaaccactcatatatccaccatctattggtatataaataaaactaacagcatcataatgaccttgtgtagtattaaaaatttgatgtctattattattaggtaatattacacttgggtaacttttattttgacttggtttgatattatgactcgaactattaatatctaaaacatttttgttttctgtgccaagtgaggaatgattatggctttctgtatcggtttttgcataagaagtatttaccattgtagtagttgctaacgctgcaattgtcattgtacatatagacat BBa_K525998_sequence 1 taatacgactcactatagggaaagaggagaaa BBa_K2144009_sequence 1 ggcggcggcggcagccttcctgaaactggcggccaccaccaccaccaccac igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z