##gff-version 3
##sequence-region BBa_K2144101 1 2097
BBa_K2144101	.	promoter	137	142	.	+	0	ID=annotation2509037;Name=-35
BBa_K2144101	.	engineered_tag	908	932	.	+	0	ID=annotation2509044;Name=barcode
BBa_K2144101	.	conserved_region	213	216	.	+	0	ID=annotation2509042;Name=conserved
BBa_K2144101	.	CDS	2077	2094	.	+	0	ID=annotation2509052;Name=Hisx6
BBa_K2144101	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation2509039;Name=LacI
BBa_K2144101	.	stop_codon	1062	1062	.	+	0	ID=annotation2509050;Name=stop
BBa_K2144101	.	polyA_site	1056	1069	.	+	0	ID=annotation2509049;Name=polya
BBa_K2144101	.	stem_loop	952	995	.	+	0	ID=annotation2509046;Name=stem_loop
BBa_K2144101	.	stem_loop	1036	1055	.	+	0	ID=annotation2509048;Name=T7 TE
BBa_K2144101	.	sequence_feature	254	842	.	+	0	ID=annotation2509043;Name=ori
BBa_K2144101	.	engineered_region	1029	1069	.	+	0	ID=annotation2509047;Name=BBa_B0012
BBa_K2144101	.	promoter	161	166	.	+	0	ID=annotation2509038;Name=-10
BBa_K2144101	.	engineered_region	1	200	.	+	0	ID=annotation2509035;Name=BBa_R0010
BBa_K2144101	.	stop_codon	2095	2097	.	+	0	ID=annotation2509054;Name=Stop
BBa_K2144101	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation2509036;Name=CAP
BBa_K2144101	.	engineered_region	941	1020	.	+	0	ID=annotation2509045;Name=BBa_B0010
BBa_K2144101	.	engineered_region	209	220	.	+	0	ID=annotation2509041;Name=BBa_B0034
BBa_K2144101	.	CDS	1140	1799	.	+	0	ID=annotation2509051;Name=CmR
BBa_K2144101	.	CDS	2059	2076	.	+	0	ID=annotation2509053;Name=Sortase A Tag
BBa_K2144101	.	start_codon	173	173	.	+	0	ID=annotation2509040;Name=start
>BBa_K2144101
gtccggcaaaaaagggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgttatgcaggc
ttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacgg
ttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaagg
ccgcgttgctggcgtttttccacaggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcg
aaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccg
cttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagtt
cggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaa
ctatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcg
aggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaagaacagtatttggtatct
gcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcgg
tggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacgggg
tctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatcc
ttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagctcgaggcttggatt
ctcaccaataaaaaacgcccggcggcaaccgagcgttctgaacaaatccagatggagttctgaggtcattactggatct
atcaacaggagtccaagcgagctcgatatcaaattacgccccgccctgccactcatcgcagtactgttgtaattcatta
agcattctgccgacatggaagccatcacaaacggcatgatgaacctgaatcgccagcggcatcagcaccttgtcgcctt
gcgtataatatttgcccatggtgaaaacgggggcgaagaagttgtccatattggccacgtttaaatcaaaactggtgaa
actcacccagggattggctgagacgaaaaacatattctcaataaaccctttagggaaataggccaggttttcaccgtaa
cacgccacatcttgcgaatatatgtgtagaaactgccggaaatcgtcgtggtattcactccagagcgatgaaaacgttt
cagtttgctcatggaaaacggtgtaacaagggtgaacactatcccatatcaccagctcaccgtctttcattgccatacg
aaattccggatgagcattcatcaggcgggcaagaatgtgaataaaggccggataaaacttgtgcttatttttctttacg
gtctttaaaaaggccgtaatatccagctgaacggtctggttataggtacattgagcaactgactgaaatgcctcaaaat
gttctttacgatgccattgggatatatcaacggtggtatatccagtgatttttttctccattttagcttccttagctcc
tgaaaatctcgataactcaaaaaatacgcccggtagtgatcttatttcattatggtgaaagttggaacctcttacgtgc
ccgatcaactcgagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacg
aggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcgcgggatccggcggcggcgg
cagccttcctgaaactggcggccatcaccatcaccatcactgatactagtagcggccgctgca