LOCUS       BBa_K2145000            2514 bp    DNA     linear       28-Jun-2024
DEFINITION  GFP/RFP reporter (Forward/Forward) with random 500bp spacer
ACCESSION   BBa_K2145000
VERSION     BBa_K2145000.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     misc_feature    63..74
                     /label=BBa_B0034
     misc_feature    67..70
                     /label=conserved
     misc_feature    81..800
                     /label=BBa_E0040
     misc_feature    809..888
                     /label=BBa_B0010
     stem_loop       820..863
                     /label=stem_loop
     misc_feature    897..937
                     /label=BBa_B0012
     stem_loop       904..923
                     /label=T7 TE
     polyA_site      924..937
                     /label=polya
     misc_feature    930..930
                     /label=stop
     misc_feature    1..937
                     /label=BBa_I13522
     misc_feature    1446..1645
                     /label=BBa_R0010
     misc_feature    1534..1571
                     /label=CAP
     promoter        1582..1587
                     /label=-35
     promoter        1606..1611
                     /label=-10
     misc_feature    1611..1645
                     /label=LacI
     misc_feature    1618..1618
                     /label=start
     misc_feature    1654..1665
                     /label=BBa_B0034
     misc_feature    1658..1661
                     /label=conserved
     misc_feature    1672..2352
                     /label=BBa_E1010
     CDS             1672..2346
                     /label=mrfp1
     misc_feature    2353..2377
                     /label=barcode
     misc_feature    2386..2465
                     /label=BBa_B0010
     stem_loop       2397..2440
                     /label=stem_loop
     misc_feature    2474..2514
                     /label=BBa_B0012
     stem_loop       2481..2500
                     /label=T7 TE
     polyA_site      2501..2514
                     /label=polya
     misc_feature    2507..2507
                     /label=stop
     misc_feature    1446..2506
                     /label=BBa_J04450
     misc_feature    938..941
                     /label=Golden Gate cloning scar
     misc_feature    942..1441
                     /label=500 bp spacer
     misc_feature    1442..1445
                     /label=Golden Gate cloning scar
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgcgtaaag gagaagaact tttcactgga gttgtcccaa
      121 ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg
      181 aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac
      241 ctgttccatg gccaacactt gtcactactt tcggttatgg tgttcaatgc tttgcgagat
      301 acccagatca tatgaaacag catgactttt tcaagagtgc catgcccgaa ggttatgtac
      361 aggaaagaac tatatttttc aaagatgacg ggaactacaa gacacgtgct gaagtcaagt
      421 ttgaaggtga tacccttgtt aatagaatcg agttaaaagg tattgatttt aaagaagatg
      481 gaaacattct tggacacaaa ttggaataca actataactc acacaatgta tacatcatgg
      541 cagacaaaca aaagaatgga atcaaagtta acttcaaaat tagacacaac attgaagatg
      601 gaagcgttca actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc
      661 ttttaccaga caaccattac ctgtccacac aatctgccct ttcgaaagat cccaacgaaa
      721 agagagacca catggtcctt cttgagtttg taacagctgc tgggattaca catggcatgg
      781 atgaactata caaataataa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg
      841 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac
      901 actggctcac cttcgggtgg gcctttctgc gtttattact ctctgaccaa aatcccgatt
      961 atagacttct caaaacataa acgaaaatat ctttcctctt gtcatctcca acaaacttcg
     1021 gaaatgcctt cacttctaca ctctcgatta aaggggacga gaagaggtcg tatatggtta
     1081 aacaatcgca tttggttagt atgcacatat tcagttgttt tgtgagtaag attttaagac
     1141 atcaatctaa agagatgatt tctcttggaa aatacactaa catttcaaaa caagcatggg
     1201 aaacatgtgg ccacttgaaa ggaaaagaca gcgagccttc tttctattcg gactatcaca
     1261 catcgtgcta atggaaggga acgtacattt ttccgtactt tctttcgttt gcccaaatgg
     1321 atgtggtggg gatttttggg tttatcttca acggatttga taatatttgc gctttatatg
     1381 tttgtgatag cacctcacag cttgagaatg gaactggtta gacatctact ttctgatccg
     1441 aggtacaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     1501 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     1561 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     1621 tgtgagcgga taacaatttc acacatacta gagaaagagg agaaatacta gatggcttcc
     1681 tccgaagacg ttatcaaaga gttcatgcgt ttcaaagttc gtatggaagg ttccgttaac
     1741 ggtcacgagt tcgaaatcga aggtgaaggt gaaggtcgtc cgtacgaagg tacccagacc
     1801 gctaaactga aagttaccaa aggtggtccg ctgccgttcg cttgggacat cctgtccccg
     1861 cagttccagt acggttccaa agcttacgtt aaacacccgg ctgacatccc ggactacctg
     1921 aaactgtcct tcccggaagg tttcaaatgg gaacgtgtta tgaacttcga agacggtggt
     1981 gttgttaccg ttacccagga ctcctccctg caagacggtg agttcatcta caaagttaaa
     2041 ctgcgtggta ccaacttccc gtccgacggt ccggttatgc agaaaaaaac catgggttgg
     2101 gaagcttcca ccgaacgtat gtacccggaa gacggtgctc tgaaaggtga aatcaaaatg
     2161 cgtctgaaac tgaaagacgg tggtcactac gacgctgaag ttaaaaccac ctacatggct
     2221 aaaaaaccgg ttcagctgcc gggtgcttac aaaaccgaca tcaaactgga catcacctcc
     2281 cacaacgaag actacaccat cgttgaacag tacgaacgtg ctgaaggtcg tcactccacc
     2341 ggtgcttaat aacgctgata gtgctagtgt agatcgctac tagagccagg catcaaataa
     2401 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
     2461 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata
//