BBa_K2145000 1 GG95 GFP/RFP reporter (Forward/Forward) with random 500bp spacer 2016-10-12T11:00:00Z 2016-10-30T08:09:51Z The GFP and RFP parts come from the iGEM GFP & RFP coding devices. This part contains two fluorescent protein coding sites (for RFP and GFP) and was assembled using Golden Gate Assembly. To make plasmid, parts were taken from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) and assembled to make new part. false false _2615_ 21363 31826 9 false This is one part of a series of parts, each with different orientations of GFP and RFP. It was used to test the expression of fluorescence under different conditions of GFP and RFP. false Laura Xinyu Chen, Melody Wu, Katie Maruna annotation2531614 1 500 bp spacer range2531614 1 942 1441 annotation2531601 1 BBa_B0034 range2531601 1 1654 1665 annotation2531612 1 BBa_J04450 range2531612 1 1446 2506 annotation2531593 1 stop range2531593 1 930 930 annotation2531604 1 mrfp1 range2531604 1 1672 2346 annotation2531585 1 BBa_B0034 range2531585 1 63 74 annotation2531613 1 Golden Gate cloning scar range2531613 1 938 941 annotation2531606 1 BBa_B0010 range2531606 1 2386 2465 annotation2531599 1 LacI range2531599 1 1611 1645 annotation2531605 1 barcode range2531605 1 2353 2377 annotation2531587 1 BBa_E0040 range2531587 1 81 800 annotation2531596 1 CAP range2531596 1 1534 1571 annotation2531590 1 BBa_B0012 range2531590 1 897 937 annotation2531598 1 -10 range2531598 1 1606 1611 annotation2531607 1 stem_loop range2531607 1 2397 2440 annotation2531610 1 polya range2531610 1 2501 2514 annotation2531588 1 BBa_B0010 range2531588 1 809 888 annotation2531586 1 conserved range2531586 1 67 70 annotation2531602 1 conserved range2531602 1 1658 1661 annotation2531592 1 polya range2531592 1 924 937 annotation2531584 1 -10 range2531584 1 43 48 annotation2531615 1 Golden Gate cloning scar range2531615 1 1442 1445 annotation2531603 1 BBa_E1010 range2531603 1 1672 2352 annotation2531600 1 start range2531600 1 1618 1618 annotation2531581 1 BBa_R0040 range2531581 1 1 54 annotation2531611 1 stop range2531611 1 2507 2507 annotation2531595 1 BBa_R0010 range2531595 1 1446 1645 annotation2531591 1 T7 TE range2531591 1 904 923 annotation2531583 1 TetR 2 range2531583 1 26 44 annotation2531608 1 BBa_B0012 range2531608 1 2474 2514 annotation2531594 1 BBa_I13522 range2531594 1 1 937 annotation2531589 1 stem_loop range2531589 1 820 863 annotation2531582 1 -35 range2531582 1 20 25 annotation2531609 1 T7 TE range2531609 1 2481 2500 annotation2531597 1 -35 range2531597 1 1582 1587 annotation2531580 1 TetR 1 range2531580 1 1 19 BBa_K2145000_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttattactctctgaccaaaatcccgattatagacttctcaaaacataaacgaaaatatctttcctcttgtcatctccaacaaacttcggaaatgccttcacttctacactctcgattaaaggggacgagaagaggtcgtatatggttaaacaatcgcatttggttagtatgcacatattcagttgttttgtgagtaagattttaagacatcaatctaaagagatgatttctcttggaaaatacactaacatttcaaaacaagcatgggaaacatgtggccacttgaaaggaaaagacagcgagccttctttctattcggactatcacacatcgtgctaatggaagggaacgtacatttttccgtactttctttcgtttgcccaaatggatgtggtggggatttttgggtttatcttcaacggatttgataatatttgcgctttatatgtttgtgatagcacctcacagcttgagaatggaactggttagacatctactttctgatccgaggtacaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z