##gff-version 3
##sequence-region BBa_K2145000 1 2514
BBa_K2145000	.	stem_loop	2481	2500	.	+	0	ID=annotation2531609;Name=T7 TE
BBa_K2145000	.	polyA_site	924	937	.	+	0	ID=annotation2531592;Name=polya
BBa_K2145000	.	stop_codon	2507	2507	.	+	0	ID=annotation2531611;Name=stop
BBa_K2145000	.	engineered_region	1672	2352	.	+	0	ID=annotation2531603;Name=BBa_E1010
BBa_K2145000	.	null	1442	1445	.	+	0	ID=annotation2531615;Name=Golden Gate cloning scar
BBa_K2145000	.	engineered_region	1	54	.	+	0	ID=annotation2531581;Name=BBa_R0040
BBa_K2145000	.	promoter	1582	1587	.	+	0	ID=annotation2531597;Name=-35
BBa_K2145000	.	promoter	1606	1611	.	+	0	ID=annotation2531598;Name=-10
BBa_K2145000	.	engineered_region	1	937	.	+	0	ID=annotation2531594;Name=BBa_I13522
BBa_K2145000	.	stem_loop	904	923	.	+	0	ID=annotation2531591;Name=T7 TE
BBa_K2145000	.	CDS	1672	2346	.	+	0	ID=annotation2531604;Name=mrfp1
BBa_K2145000	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation2531583;Name=TetR 2
BBa_K2145000	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation2531580;Name=TetR 1
BBa_K2145000	.	engineered_region	1446	1645	.	+	0	ID=annotation2531595;Name=BBa_R0010
BBa_K2145000	.	engineered_region	2474	2514	.	+	0	ID=annotation2531608;Name=BBa_B0012
BBa_K2145000	.	non_covalent_binding_site	1611	1645	.	+	0	ID=annotation2531599;Name=LacI
BBa_K2145000	.	stem_loop	820	863	.	+	0	ID=annotation2531589;Name=stem_loop
BBa_K2145000	.	engineered_region	1446	2506	.	+	0	ID=annotation2531612;Name=BBa_J04450
BBa_K2145000	.	sequence_feature	942	1441	.	+	0	ID=annotation2531614;Name=500 bp spacer
BBa_K2145000	.	null	938	941	.	+	0	ID=annotation2531613;Name=Golden Gate cloning scar
BBa_K2145000	.	engineered_region	897	937	.	+	0	ID=annotation2531590;Name=BBa_B0012
BBa_K2145000	.	promoter	43	48	.	+	0	ID=annotation2531584;Name=-10
BBa_K2145000	.	engineered_region	63	74	.	+	0	ID=annotation2531585;Name=BBa_B0034
BBa_K2145000	.	engineered_region	81	800	.	+	0	ID=annotation2531587;Name=BBa_E0040
BBa_K2145000	.	engineered_region	1654	1665	.	+	0	ID=annotation2531601;Name=BBa_B0034
BBa_K2145000	.	engineered_region	2386	2465	.	+	0	ID=annotation2531606;Name=BBa_B0010
BBa_K2145000	.	promoter	20	25	.	+	0	ID=annotation2531582;Name=-35
BBa_K2145000	.	polyA_site	2501	2514	.	+	0	ID=annotation2531610;Name=polya
BBa_K2145000	.	conserved_region	1658	1661	.	+	0	ID=annotation2531602;Name=conserved
BBa_K2145000	.	stop_codon	930	930	.	+	0	ID=annotation2531593;Name=stop
BBa_K2145000	.	stem_loop	2397	2440	.	+	0	ID=annotation2531607;Name=stem_loop
BBa_K2145000	.	non_covalent_binding_site	1534	1571	.	+	0	ID=annotation2531596;Name=CAP
BBa_K2145000	.	engineered_region	809	888	.	+	0	ID=annotation2531588;Name=BBa_B0010
BBa_K2145000	.	conserved_region	67	70	.	+	0	ID=annotation2531586;Name=conserved
BBa_K2145000	.	start_codon	1618	1618	.	+	0	ID=annotation2531600;Name=start
BBa_K2145000	.	engineered_tag	2353	2377	.	+	0	ID=annotation2531605;Name=barcode
>BBa_K2145000
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaa
ttttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaac
tacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaa
acagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaac
tacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaag
aagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaa
gaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaa
aatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatc
ccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactata
caaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttg
tttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttattactctctgacc
aaaatcccgattatagacttctcaaaacataaacgaaaatatctttcctcttgtcatctccaacaaacttcggaaatgc
cttcacttctacactctcgattaaaggggacgagaagaggtcgtatatggttaaacaatcgcatttggttagtatgcac
atattcagttgttttgtgagtaagattttaagacatcaatctaaagagatgatttctcttggaaaatacactaacattt
caaaacaagcatgggaaacatgtggccacttgaaaggaaaagacagcgagccttctttctattcggactatcacacatc
gtgctaatggaagggaacgtacatttttccgtactttctttcgtttgcccaaatggatgtggtggggatttttgggttt
atcttcaacggatttgataatatttgcgctttatatgtttgtgatagcacctcacagcttgagaatggaactggttaga
catctactttctgatccgaggtacaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcac
gacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccagg
ctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagag
gagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgtta
acggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttac
caaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacac
ccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacg
gtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaa
cttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagac
ggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacct
acatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaaga
ctacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgt
agatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgttt
gtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata