BBa_K2145002 1 GG97 GFP/RFP reporter with spacer (500bp) 2016-10-13T11:00:00Z 2016-10-19T02:59:37Z Constructed from BBa_J04450, BBa_I13522, and a spacer derived from a concatenation of several non-functional plasmid backbone sequences. This part contains two fluorescent protein coding sites (for RFP and GFP) and was assembled using Golden Gate Assembly. To make plasmid, parts were taken from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) along with a "spacer" part (500bp) and pSB1C3 backbone assembled to make the final part. It can be used to express both GFP and RFP proteins. true false _2615_ 31829 31837 9 false Constructed with Golden Gate assembly. false Melody Wu annotation2511882 1 BBa_B0012 range2511882 1 1029 1069 annotation2511886 1 BBa_J04450 range2511886 1 1 1069 annotation2511900 1 stop range2511900 1 2007 2007 annotation2511874 1 start range2511874 1 173 173 annotation2511897 1 BBa_B0012 range2511897 1 1974 2014 annotation2511884 1 polya range2511884 1 1056 1069 annotation2511894 1 BBa_E0040 range2511894 1 1158 1877 annotation2511883 1 T7 TE range2511883 1 1036 1055 annotation2511881 1 stem_loop range2511881 1 952 995 annotation2511898 1 T7 TE range2511898 1 1981 2000 annotation2511878 1 mrfp1 range2511878 1 227 901 annotation2511871 1 -35 range2511871 1 137 142 annotation2511890 1 TetR 2 range2511890 1 1103 1121 annotation2511870 1 CAP range2511870 1 89 126 annotation2511873 1 LacI range2511873 1 166 200 annotation2511893 1 conserved range2511893 1 1144 1147 annotation2511891 1 -10 range2511891 1 1120 1125 annotation2511879 1 barcode range2511879 1 908 932 annotation2511887 1 TetR 1 range2511887 1 1078 1096 annotation2511901 1 BBa_I13522 range2511901 1 1078 2014 annotation2511888 1 BBa_R0040 range2511888 1 1078 1131 annotation2511875 1 BBa_B0034 range2511875 1 209 220 annotation2511869 1 BBa_R0010 range2511869 1 1 200 annotation2511895 1 BBa_B0010 range2511895 1 1886 1965 annotation2511892 1 BBa_B0034 range2511892 1 1140 1151 annotation2511876 1 conserved range2511876 1 213 216 annotation2511877 1 BBa_E1010 range2511877 1 227 907 annotation2511872 1 -10 range2511872 1 161 166 annotation2511880 1 BBa_B0010 range2511880 1 941 1020 annotation2511896 1 stem_loop range2511896 1 1897 1940 annotation2511899 1 polya range2511899 1 2001 2014 annotation2511889 1 -35 range2511889 1 1097 1102 annotation2511885 1 stop range2511885 1 1062 1062 BBa_K2145002_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z