BBa_K2145005 1 BBa_K2145005 GFP/RFP reporter with spacer part (532bp) 2016-10-13T11:00:00Z 2016-10-14T10:10:17Z Constructed from BBa_J04450, BBa_I13522, and a spacer derived from a concatenation of several non-functional plasmid backbone sequences. This plasmid contains a GFP and an RFP reporter separated by a 532-bp spacer containing three gRNA binding sites for dCas. The reporter is designed to demonstrate the effect, if any, of binding a dCas "clamp" (or a random sequence in) between two genes (the idea being that such a clamp can limit propagation of supercoiling between the genes, functionally isolating them). true false _2615_ 31826 31826 9 false Constructed with Golden Gate Assembly. false Laura Xinyu Chen igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z