LOCUS       BBa_K2145100            2352 bp    DNA     linear       28-Jun-2024
DEFINITION  GFP/RFP reporter (Forward/Forward) separated by three dCas clamp 
            sites
ACCESSION   BBa_K2145100
VERSION     BBa_K2145100.1  
FEATURES             Location/Qualifiers
     misc_feature    45..981
                     /label=BBa_I13522
     misc_feature    986..1235
                     /label=spacer
     misc_feature    1025..1047
                     /label=gRNA_268
     misc_feature    1081..1103
                     /label=gRNA_355
     misc_feature    1148..1170
                     /label=gRNA_525
     misc_feature    1240..2308
                     /label=BBa_J04450
ORIGIN
        1 gctgggagtt cgtagacgga aacaaacgca gaatccaagc ggagtcccta tcagtgatag
       61 agattgacat ccctatcagt gatagagata ctgagcacta ctagagaaag aggagaaata
      121 ctagatgcgt aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga
      181 tggtgatgtt aatgggcaca aattttctgt cagtggagag ggtgaaggtg atgcaacata
      241 cggaaaactt acccttaaat ttatttgcac tactggaaaa ctacctgttc catggccaac
      301 acttgtcact actttcggtt atggtgttca atgctttgcg agatacccag atcatatgaa
      361 acagcatgac tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa gaactatatt
      421 tttcaaagat gacgggaact acaagacacg tgctgaagtc aagtttgaag gtgataccct
      481 tgttaataga atcgagttaa aaggtattga ttttaaagaa gatggaaaca ttcttggaca
      541 caaattggaa tacaactata actcacacaa tgtatacatc atggcagaca aacaaaagaa
      601 tggaatcaaa gttaacttca aaattagaca caacattgaa gatggaagcg ttcaactagc
      661 agaccattat caacaaaata ctccaattgg cgatggccct gtccttttac cagacaacca
      721 ttacctgtcc acacaatctg ccctttcgaa agatcccaac gaaaagaggg accacatggt
      781 ccttcttgag tttgtaacag ctgctgggat tacacatggc atggatgaac tatacaaata
      841 ataatactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
      901 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg
      961 gtgggccttt ctgcgtttat atactgatta tagacttctc aattttgtca tctccaacaa
     1021 acttacacca tgagcaatag caattggcgg aaatgccttc acttctacac tctcgattaa
     1081 aagaggtaca gctttcgcaa aggaggggac gagaagaggt cgtatatggt taaacaatcg
     1141 catttggtaa atcagcaaaa ctggcggagg ttagtatgca catattcagt tgaatctaaa
     1201 gaacactaac atttcaaaac aagcatggga aacataggtc aatacgcaaa ccgcctctcc
     1261 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     1321 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     1381 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacat
     1441 actagagaaa gaggagaaat actagatggc ttcctccgaa gacgttatca aagagttcat
     1501 gcgtttcaaa gttcgtatgg aaggttccgt taacggtcac gagttcgaaa tcgaaggtga
     1561 aggtgaaggt cgtccgtacg aaggtaccca gaccgctaaa ctgaaagtta ccaaaggtgg
     1621 tccgctgccg ttcgcttggg acatcctgtc cccgcagttc cagtacggtt ccaaagctta
     1681 cgttaaacac ccggctgaca tcccggacta cctgaaactg tccttcccgg aaggtttcaa
     1741 atgggaacgt gttatgaact tcgaagacgg tggtgttgtt accgttaccc aggactcctc
     1801 cctgcaagac ggtgagttca tctacaaagt taaactgcgt ggtaccaact tcccgtccga
     1861 cggtccggtt atgcagaaaa aaaccatggg ttgggaagct tccaccgaac gtatgtaccc
     1921 ggaagacggt gctctgaaag gtgaaatcaa aatgcgtctg aaactgaaag acggtggtca
     1981 ctacgacgct gaagttaaaa ccacctacat ggctaaaaaa ccggttcagc tgccgggtgc
     2041 ttacaaaacc gacatcaaac tggacatcac ctcccacaac gaagactaca ccatcgttga
     2101 acagtacgaa cgtgctgaag gtcgtcactc caccggtgct taataacgct gatagtgcta
     2161 gtgtagatcg ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg
     2221 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca
     2281 ccttcgggtg ggcctttctg cgtttatagc ttgcactgaa ggtcctcaat cgcactggaa
     2341 acatcaaggt cg
//