LOCUS       BBa_K2145101            2352 bp    DNA     linear       22-Dec-2024
DEFINITION  GFP/RFP reporter (Reverse/Forward) separated by three dCas clamp 
            sites
ACCESSION   BBa_K2145101
VERSION     BBa_K2145101.1  
FEATURES             Location/Qualifiers
     misc_feature    182..901
                     /label=BBa_I13522
     misc_feature    986..1235
                     /label=spacer
     misc_feature    1025..1047
                     /label=gRNA_268
     misc_feature    1081..1103
                     /label=gRNA_355
     misc_feature    1148..1170
                     /label=gRNA_525
     misc_feature    1240..2308
                     /label=BBa_J04450
ORIGIN
        1 gctgggagtt cgtagacgga aacaaacgca gaatccaagc ggagtataaa cgcagaaagg
       61 cccacccgaa ggtgagccag tgtgactcta gtagagagcg ttcaccgaca aacaacagat
      121 aaaacgaaag gcccagtctt tcgactgagc ctttcgtttt atttgatgcc tggctctagt
      181 attattattt gtatagttca tccatgccat gtgtaatccc agcagctgtt acaaactcaa
      241 gaaggaccat gtggtccctc ttttcgttgg gatctttcga aagggcagat tgtgtggaca
      301 ggtaatggtt gtctggtaaa aggacagggc catcgccaat tggagtattt tgttgataat
      361 ggtctgctag ttgaacgctt ccatcttcaa tgttgtgtct aattttgaag ttaactttga
      421 ttccattctt ttgtttgtct gccatgatgt atacattgtg tgagttatag ttgtattcca
      481 atttgtgtcc aagaatgttt ccatcttctt taaaatcaat accttttaac tcgattctat
      541 taacaagggt atcaccttca aacttgactt cagcacgtgt cttgtagttc ccgtcatctt
      601 tgaaaaatat agttctttcc tgtacataac cttcgggcat ggcactcttg aaaaagtcat
      661 gctgtttcat atgatctggg tatctcgcaa agcattgaac accataaccg aaagtagtga
      721 caagtgttgg ccatggaaca ggtagttttc cagtagtgca aataaattta agggtaagtt
      781 ttccgtatgt tgcatcacct tcaccctctc cactgacaga aaatttgtgc ccattaacat
      841 caccatctaa ttcaacaaga attgggacaa ctccagtgaa aagttcttct cctttacgca
      901 tctagtattt ctcctctttc tctagtagtg ctcagtatct ctatcactga tagggatgtc
      961 aatctctatc actgataggg atactgatta tagacttctc aattttgtca tctccaacaa
     1021 acttacacca tgagcaatag caattggcgg aaatgccttc acttctacac tctcgattaa
     1081 aagaggtaca gctttcgcaa aggaggggac gagaagaggt cgtatatggt taaacaatcg
     1141 catttggtaa atcagcaaaa ctggcggagg ttagtatgca catattcagt tgaatctaaa
     1201 gaacactaac atttcaaaac aagcatggga aacataggtc aatacgcaaa ccgcctctcc
     1261 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     1321 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     1381 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacat
     1441 actagagaaa gaggagaaat actagatggc ttcctccgaa gacgttatca aagagttcat
     1501 gcgtttcaaa gttcgtatgg aaggttccgt taacggtcac gagttcgaaa tcgaaggtga
     1561 aggtgaaggt cgtccgtacg aaggtaccca gaccgctaaa ctgaaagtta ccaaaggtgg
     1621 tccgctgccg ttcgcttggg acatcctgtc cccgcagttc cagtacggtt ccaaagctta
     1681 cgttaaacac ccggctgaca tcccggacta cctgaaactg tccttcccgg aaggtttcaa
     1741 atgggaacgt gttatgaact tcgaagacgg tggtgttgtt accgttaccc aggactcctc
     1801 cctgcaagac ggtgagttca tctacaaagt taaactgcgt ggtaccaact tcccgtccga
     1861 cggtccggtt atgcagaaaa aaaccatggg ttgggaagct tccaccgaac gtatgtaccc
     1921 ggaagacggt gctctgaaag gtgaaatcaa aatgcgtctg aaactgaaag acggtggtca
     1981 ctacgacgct gaagttaaaa ccacctacat ggctaaaaaa ccggttcagc tgccgggtgc
     2041 ttacaaaacc gacatcaaac tggacatcac ctcccacaac gaagactaca ccatcgttga
     2101 acagtacgaa cgtgctgaag gtcgtcactc caccggtgct taataacgct gatagtgcta
     2161 gtgtagatcg ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg
     2221 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca
     2281 ccttcgggtg ggcctttctg cgtttatagc ttgcactgaa ggtcctcaat cgcactggaa
     2341 acatcaaggt cg
//