LOCUS       BBa_K2145103            2352 bp    DNA     linear       22-Nov-2024
DEFINITION  GFP/RFP reporter (Reverse/Reverse) separated by three dCas clamp 
            sites
ACCESSION   BBa_K2145103
VERSION     BBa_K2145103.1  
FEATURES             Location/Qualifiers
     misc_feature    1148..1170
                     /label=gRNA_525
     misc_feature    45..981
                     /label=BBa_I13522
     misc_feature    986..1235
                     /label=spacer
     misc_feature    1025..1047
                     /label=gRNA_268
     misc_feature    1081..1103
                     /label=gRNA_355
     misc_feature    1240..2308
                     /label=BBa_J04450
ORIGIN
        1 gctgggagtt cgtagacgga aacaaacgca gaatccaagc ggagtataaa cgcagaaagg
       61 cccacccgaa ggtgagccag tgtgactcta gtagagagcg ttcaccgaca aacaacagat
      121 aaaacgaaag gcccagtctt tcgactgagc ctttcgtttt atttgatgcc tggctctagt
      181 attattattt gtatagttca tccatgccat gtgtaatccc agcagctgtt acaaactcaa
      241 gaaggaccat gtggtccctc ttttcgttgg gatctttcga aagggcagat tgtgtggaca
      301 ggtaatggtt gtctggtaaa aggacagggc catcgccaat tggagtattt tgttgataat
      361 ggtctgctag ttgaacgctt ccatcttcaa tgttgtgtct aattttgaag ttaactttga
      421 ttccattctt ttgtttgtct gccatgatgt atacattgtg tgagttatag ttgtattcca
      481 atttgtgtcc aagaatgttt ccatcttctt taaaatcaat accttttaac tcgattctat
      541 taacaagggt atcaccttca aacttgactt cagcacgtgt cttgtagttc ccgtcatctt
      601 tgaaaaatat agttctttcc tgtacataac cttcgggcat ggcactcttg aaaaagtcat
      661 gctgtttcat atgatctggg tatctcgcaa agcattgaac accataaccg aaagtagtga
      721 caagtgttgg ccatggaaca ggtagttttc cagtagtgca aataaattta agggtaagtt
      781 ttccgtatgt tgcatcacct tcaccctctc cactgacaga aaatttgtgc ccattaacat
      841 caccatctaa ttcaacaaga attgggacaa ctccagtgaa aagttcttct cctttacgca
      901 tctagtattt ctcctctttc tctagtagtg ctcagtatct ctatcactga tagggatgtc
      961 aatctctatc actgataggg atactgatta tagacttctc aattttgtca tctccaacaa
     1021 acttacacca tgagcaatag caattggcgg aaatgccttc acttctacac tctcgattaa
     1081 aagaggtaca gctttcgcaa aggaggggac gagaagaggt cgtatatggt taaacaatcg
     1141 catttggtaa atcagcaaaa ctggcggagg ttagtatgca catattcagt tgaatctaaa
     1201 gaacactaac atttcaaaac aagcatggga aacataggtt ataaacgcag aaaggcccac
     1261 ccgaaggtga gccagtgtga ctctagtaga gagcgttcac cgacaaacaa cagataaaac
     1321 gaaaggccca gtctttcgac tgagcctttc gttttatttg atgcctggct ctagtagcga
     1381 tctacactag cactatcagc gttattaagc accggtggag tgacgacctt cagcacgttc
     1441 gtactgttca acgatggtgt agtcttcgtt gtgggaggtg atgtccagtt tgatgtcggt
     1501 tttgtaagca cccggcagct gaaccggttt tttagccatg taggtggttt taacttcagc
     1561 gtcgtagtga ccaccgtctt tcagtttcag acgcattttg atttcacctt tcagagcacc
     1621 gtcttccggg tacatacgtt cggtggaagc ttcccaaccc atggtttttt tctgcataac
     1681 cggaccgtcg gacgggaagt tggtaccacg cagtttaact ttgtagatga actcaccgtc
     1741 ttgcagggag gagtcctggg taacggtaac aacaccaccg tcttcgaagt tcataacacg
     1801 ttcccatttg aaaccttccg ggaaggacag tttcaggtag tccgggatgt cagccgggtg
     1861 tttaacgtaa gctttggaac cgtactggaa ctgcggggac aggatgtccc aagcgaacgg
     1921 cagcggacca cctttggtaa ctttcagttt agcggtctgg gtaccttcgt acggacgacc
     1981 ttcaccttca ccttcgattt cgaactcgtg accgttaacg gaaccttcca tacgaacttt
     2041 gaaacgcatg aactctttga taacgtcttc ggaggaagcc atctagtatt tctcctcttt
     2101 ctctagtatg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     2161 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     2221 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     2281 cgcgcgggga gaggcggttt gcgtattggc ttgcactgaa ggtcctcaat cgcactggaa
     2341 acatcaaggt cg
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