BBa_K2145124 1 GG99 GFP/RFP reporter (Forward/Reverse) with random 500bp spacer 2016-10-17T11:00:00Z 2016-10-30T06:22:18Z The RFP part is BBa_J04450 from the iGEM registry and the GFP part is BBa_I13522 from the iGEM registry. The backbone is a pSB1C3 backbone and the spacer is derived from a number of different backbones. This part contains two fluorescent protein coding sites (RFP and GFP) and was assembled using Golden Gate Assembly. In order to make the plasmid, parts were taken from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) along with a 500 base pair "spacer" part derived from different backbones, and a pSB1C3 backbone assembled to make the final part. It can be used to express both GFP and RFP proteins. false false _2615_ 31903 31829 9 false This plasmid was assembled using Golden Gate Assembly. false Katie Maruna, Laura Chen, Melody Wu annotation2519787 1 BBa_I13522 range2519787 1 1622 2558 annotation2519785 1 500 bp spacer range2519785 1 1118 1617 annotation2519783 1 BBa_J04450 range2519783 1 45 1113 BBa_K2145124_sequence 1 gctgggagttcgtagacggaaacaaacgcagaatccaagcggagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactctctgaccaaaatcccgattatagacttctcaaaacataaacgaaaatatctttcctcttgtcatctccaacaaacttcggaaatgccttcacttctacactctcgattaaaggggacgagaagaggtcgtatatggttaaacaatcgcatttggttagtatgcacatattcagttgttttgtgagtaagattttaagacatcaatctaaagagatgatttctcttggaaaatacactaacatttcaaaacaagcatgggaaacatgtggccacttgaaaggaaaagacagcgagccttctttctattcggactatcacacatcgtgctaatggaagggaacgtacatttttccgtactttctttcgtttgcccaaatggatgtggtggggatttttgggtttatcttcaacggatttgataatatttgcgctttatatgtttgtgatagcacctcacagcttgagaatggaactggttagacatctactttctgatccgaggttccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagggaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatagcttgcactgaaggtcctcaatcgcactggaaacatcaaggtcg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z