LOCUS BBa_K2145998 4438 bp DNA linear 01-Jul-2024 DEFINITION test ACCESSION BBa_K2145998 VERSION BBa_K2145998.1 FEATURES Location/Qualifiers misc_feature 18..324 /label=f1 origin misc_feature 1..455 /label=BBa_K314110 misc_feature 462..983 /label=5' AmyE homologous sequence misc_feature 462..983 /label=BBa_K143001 promoter 1054..1059 /label=Sigma A-35 promoter 1077..1082 /label=Sigma A -10 promoter 992..1088 /label=BBa_K143012 RBS 1097..1108 /label=rbs RBS 1097..1108 /label=BBa_K143021 misc_feature 992..1108 /label=BBa_K143053 CDS 1115..1879 /label=Aad9 Spectinomycin Acetyltransferase misc_feature 1880..1882 /label=stop misc_feature 1883..1885 /label=stop misc_feature 1115..1117 /label=start CDS 1115..1885 /label=BBa_K143031 stem_loop 1905..1948 /label=stem_loop misc_feature 1894..1973 /label=BBa_B0010 terminator 1894..1973 /label=BBa_B0010 stem_loop 1989..2008 /label=T7 TE misc_feature 2015..2015 /label=stop polyA_site 2009..2022 /label=polya misc_feature 1982..2022 /label=BBa_B0012 terminator 1982..2022 /label=BBa_B0012 misc_feature 1115..2022 /label=BBa_K143065 promoter 2093..2098 /label=Sigma A-35 promoter 2116..2121 /label=Sigma A -10 promoter 2031..2127 /label=BBa_K143012 RBS 2136..2147 /label=rbs RBS 2136..2147 /label=BBa_K143021 misc_feature 2031..2147 /label=BBa_K143053 CDS 2154..3233 /label=LacI (Lva-, N-terminal deletion) regulatory protein misc_feature 2154..2156 /label=start misc_feature 3234..3236 /label=stop misc_feature 3237..3239 /label=stop CDS 2154..3239 /label=BBa_K143033 stem_loop 3259..3302 /label=stem_loop misc_feature 3248..3327 /label=BBa_B0010 terminator 3248..3327 /label=BBa_B0010 stem_loop 3343..3362 /label=T7 TE misc_feature 3369..3369 /label=stop polyA_site 3363..3376 /label=polya misc_feature 3336..3376 /label=BBa_B0012 terminator 3336..3376 /label=BBa_B0012 misc_feature 2154..3376 /label=BBa_K143062 misc_feature 3385..3412 /label=BBa_K316002 misc_feature 3421..3424 /label=5' product misc_feature 3425..3428 /label=3' product misc_feature 3421..3428 /label=BBa_K316013 misc_feature 3385..3428 /label=BBa_K316014 misc_feature 3437..4441 /label=3' AmyE homologous sequence misc_feature 3437..4438 /label=BBa_K143002 misc_feature 462..4438 /label=BBa_K316027 ORIGIN 1 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg 61 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca 121 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta 181 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc 241 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg 301 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat 361 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta 421 acgcgaattt taacaaaata ttaacgttta caatttacta gatgtttgca aaacgattca 481 aaacctcttt actgccgtta ttcgctggat ttttattgct gtttcatttg gttctggcag 541 gaccggcggc tgcgagtgct gaaacggcga acaaatcgaa tgagcttaca gcaccgtcga 601 tcaaaagcgg aaccattctt catgcatgga attggtcgtt caatacgtta aaacacaata 661 tgaaggatat tcatgatgca ggatatacag ccattcagac atctccgatt aaccaagtaa 721 aggaagggaa tcaaggagat aaaagcatgt cgaactggta ctggctgtat cagccgacat 781 cgtatcaaat tggcaaccgt tacttaggta ctgaacaaga atttaaagaa atgtgtgcag 841 ccgctgaaga atatggcata aaggtcattg ttgacgcggt catcaatcat accaccagtg 901 attatgccgc gatttccaat gaggttaaga gtattccaaa ctggacacat ggaaacacac 961 aaattaaaaa ctggtctgat cgatactaga gaattttgtc aaaataattt tattgacaac 1021 gtcttattaa cgttgatata atttaaattt tatttgacaa aaatgggctc gtgttgtaca 1081 ataaatgtta ctagagaaag gtggtgaata ctagatgagg aggatatatt tgaatacata 1141 cgaacaaatt aataaagtga aaaaaatact tcggaaacat ttaaaaaata accttattgg 1201 tacttacatg tttggatcag gagttgagag tggactaaaa ccaaatagtg atcttgactt 1261 tttagtcgtc gtatctgaac cattgacaga tcaaagtaaa gaaatactta tacaaaaaat 1321 tagacctatt tcaaaaaaaa taggagataa aagcaactta cgatatattg aattaacaat 1381 tattattcag caagaaatgg taccgtggaa tcatcctccc aaacaagaat ttatttatgg 1441 agaatggtta caagagcttt atgaacaagg atacattcct cagaaggaat taaattcaga 1501 tttaaccata atgctttacc aagcaaaacg aaaaaataaa agaatatacg gaaattatga 1561 cttagaggaa ttactacctg atattccatt ttctgatgtg agaagagcca ttatggattc 1621 gtcagaggaa ttaatagata attatcagga tgatgaaacc aactctatat taactttatg 1681 ccgtatgatt ttaactatgg acacgggtaa aatcatacca aaagatattg cgggaaatgc 1741 agtggctgaa tcttctccat tagaacatag ggagagaatt ttgttagcag ttcgtagtta 1801 tcttggagag aatattgaat ggactaatga aaatgtaaat ttaactataa actatttaaa 1861 taacagatta aaaaaattat aataatacta gagccaggca tcaaataaaa cgaaaggctc 1921 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga 1981 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tatactagag aattttgtca 2041 aaataatttt attgacaacg tcttattaac gttgatataa tttaaatttt atttgacaaa 2101 aatgggctcg tgttgtacaa taaatgttac tagagaaagg tggtgaatac tagatgaaac 2161 cagtaacgtt atacgatgtc gcagagtatg ccggtgtctc ttatcagacc gtttcccgcg 2221 tggtgaacca ggccagccac gtttctgcga aaacgcggga aaaagtggaa gcggcgatgg 2281 cggagctgaa ttacattccc aaccgcgtgg cacaacaact ggcgggcaaa cagtcgttgc 2341 tgattggcgt tgccacctcc agtctggccc tgcacgcgcc gtcgcaaatt gtcgcggcga 2401 ttaaatctcg cgccgatcaa ctgggtgcca gcgtggtggt gtcgatggta gaacgaagcg 2461 gcgtcgaagc ctgtaaaacg gcggtgcaca atcttctcgc gcaacgcgtc agtgggctga 2521 tcattaacta tccgctggat gaccaggatg ccattgctgt ggaagctgcc tgcactaatg 2581 ttccggcgtt atttcttgat gtctctgacc agacacccat caacagtatt attttctccc 2641 atgaagacgg tacgcgactg ggcgtggagc atctggtcgc attgggtcac cagcaaatcg 2701 cgctgttagc gggcccatta agttctgtct cggcgcgtct gcgtctggct ggctggcata 2761 aatatctcac tcgcaatcaa attcagccga tagcggaacg ggaaggcgac tggagtgcca 2821 tgtccggttt tcaacaaacc atgcaaatgc tgaatgaggg catcgttccc actgcgatgc 2881 tggttgccaa cgatcagatg gcgctgggcg caatgcgcgc cattaccgag tccgggctgc 2941 gcgttggtgc ggatatctcg gtagtgggat acgacgatac cgaagacagc tcatgttata 3001 tcccgccgtc aaccaccatc aaacaggatt ttcgcctgct ggggcaaacc agcgtggacc 3061 gcttgctgca actctctcag ggccaggcgg tgaagggcaa tcagctgttg cccgtctcac 3121 tggtgaaaag aaaaaccacc ctggcgccca atacgcaaac cgcctctccc cgcgcgttgg 3181 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtaataat 3241 actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt 3301 atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg 3361 cctttctgcg tttatatact agagatctcc tagaatatat attatgtaaa cttactagag 3421 gaaatttcta ctagagatcc gtttaggctg ggcggtgata gcttctcgtt caggcagtac 3481 gcctcttttc ttttccagac ctgagggagg cggaaatggt gtgaggttcc cggggaaaag 3541 ccaaataggc gatcgcggga gtgctttatt tgaagatcag gctatcactg cggtcaatag 3601 atttcacaat gtgatggctg gacagcctga ggaactctcg aacccgaatg gaaacaacca 3661 gatatttatg aatcagcgcg gctcacatgg cgttgtgctg gcaaatgcag gttcatcctc 3721 tgtctctatc aatacggcaa caaaattgcc tgatggcagg tatgacaata aagctggagc 3781 gggttcattt caagtgaacg atggtaaact gacaggcacg atcaatgcca ggtctgtagc 3841 tgtgctttat cctgatgata ttgcaaaagc gcctcatgtt ttccttgaga attacaaaac 3901 aggtgtaaca cattctttca atgatcaact gacgattacc ttgcgtgcag atgcgaatac 3961 aacaaaagcc gtttatcaaa tcaataatgg accagagacg gcgtttaagg atggagatca 4021 attcacaatc ggaaaaggag atccatttgg caaaacatac accatcatgt taaaaggaac 4081 gaacagtgat ggtgtaacga ggaccgagaa atacagtttt gttaaaagag atccagcgtc 4141 ggccaaaacc atcggctatc aaaatccgaa tcattggagc caggtaaatg cttatatcta 4201 taaacatgat gggagccgag taattgaatt gaccggatct tggcctggaa aaccaatgac 4261 taaaaatgca gacggaattt acacgctgac gctgcctgcg gacacggata caaccaacgc 4321 aaaagtgatt tttaataatg gcagcgccca agtgcccggt cagaatcagc ctggctttga 4381 ttacgtgcta aatggtttat ataatgactc gggcttaagc ggttctcttc cccattga //