##gff-version 3
##sequence-region BBa_K2145998 1 4438
BBa_K2145998	.	engineered_region	462	4438	.	+	0	ID=BBa_K316027;Name=BBa_K316027
BBa_K2145998	.	sequence_feature	462	983	.	+	0	ID=BBa_K143001;Name=BBa_K143001;Parent=BBa_K316027
BBa_K2145998	.	sequence_feature	462	983	.	+	0	ID=annotation1974145;Name=5' AmyE homologous sequence;Parent=BBa_K143001
BBa_K2145998	.	sequence_feature	3437	4438	.	+	0	ID=BBa_K143002;Name=BBa_K143002;Parent=BBa_K316027
BBa_K2145998	.	sequence_feature	3437	4441	.	+	0	ID=annotation1974146;Name=3' AmyE homologous sequence;Parent=BBa_K143002
BBa_K2145998	.	engineered_region	2154	3376	.	+	0	ID=BBa_K143062;Name=BBa_K143062;Parent=BBa_K316027
BBa_K2145998	.	terminator	3336	3376	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K143062
BBa_K2145998	.	polyA_site	3363	3376	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K2145998	.	engineered_region	3336	3376	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K2145998	.	stem_loop	3343	3362	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K2145998	.	stop_codon	3369	3369	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K2145998	.	terminator	3248	3327	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K143062
BBa_K2145998	.	stem_loop	3259	3302	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K2145998	.	engineered_region	3248	3327	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K2145998	.	CDS	2154	3239	.	+	0	ID=BBa_K143033;Name=BBa_K143033;Parent=BBa_K143062
BBa_K2145998	.	stop_codon	3234	3236	.	+	0	ID=annotation1994271;Name=stop;Parent=BBa_K143033
BBa_K2145998	.	start_codon	2154	2156	.	+	0	ID=annotation1992702;Name=start;Parent=BBa_K143033
BBa_K2145998	.	stop_codon	3237	3239	.	+	0	ID=annotation1994272;Name=stop;Parent=BBa_K143033
BBa_K2145998	.	CDS	2154	3233	.	+	0	ID=annotation1975974;Name=LacI (Lva-, N-terminal deletion) regulatory protein;Parent=BBa_K143033
BBa_K2145998	.	engineered_region	1115	2022	.	+	0	ID=BBa_K143065;Name=BBa_K143065;Parent=BBa_K316027
BBa_K2145998	.	terminator	1982	2022	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K143065
BBa_K2145998	.	polyA_site	2009	2022	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K2145998	.	engineered_region	1982	2022	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K2145998	.	stem_loop	1989	2008	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K2145998	.	stop_codon	2015	2015	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K2145998	.	terminator	1894	1973	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K143065
BBa_K2145998	.	stem_loop	1905	1948	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K2145998	.	engineered_region	1894	1973	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K2145998	.	CDS	1115	1885	.	+	0	ID=BBa_K143031;Name=BBa_K143031;Parent=BBa_K143065
BBa_K2145998	.	CDS	1115	1879	.	+	0	ID=annotation1975961;Name=Aad9 Spectinomycin Acetyltransferase;Parent=BBa_K143031
BBa_K2145998	.	stop_codon	1880	1882	.	+	0	ID=annotation1992696;Name=stop;Parent=BBa_K143031
BBa_K2145998	.	stop_codon	1883	1885	.	+	0	ID=annotation1992697;Name=stop;Parent=BBa_K143031
BBa_K2145998	.	start_codon	1115	1117	.	+	0	ID=annotation1992698;Name=start;Parent=BBa_K143031
BBa_K2145998	.	engineered_region	992	1108	.	+	0	ID=BBa_K143053;Name=BBa_K143053;Parent=BBa_K316027
BBa_K2145998	.	ribosome_entry_site	1097	1108	.	+	0	ID=BBa_K143021;Name=BBa_K143021;Parent=BBa_K143053
BBa_K2145998	.	ribosome_entry_site	1097	1108	.	+	0	ID=annotation1975997;Name=rbs;Parent=BBa_K143021
BBa_K2145998	.	promoter	992	1088	.	+	0	ID=BBa_K143012;Name=BBa_K143012;Parent=BBa_K143053
BBa_K2145998	.	promoter	1054	1059	.	+	0	ID=annotation1975704;Name=Sigma A-35;Parent=BBa_K143012
BBa_K2145998	.	promoter	1077	1082	.	+	0	ID=annotation1975705;Name=Sigma A -10;Parent=BBa_K143012
BBa_K2145998	.	engineered_region	2031	2147	.	+	0	ID=BBa_K143053;Name=BBa_K143053;Parent=BBa_K316027
BBa_K2145998	.	ribosome_entry_site	2136	2147	.	+	0	ID=BBa_K143021;Name=BBa_K143021;Parent=BBa_K143053
BBa_K2145998	.	ribosome_entry_site	2136	2147	.	+	0	ID=annotation1975997;Name=rbs;Parent=BBa_K143021
BBa_K2145998	.	promoter	2031	2127	.	+	0	ID=BBa_K143012;Name=BBa_K143012;Parent=BBa_K143053
BBa_K2145998	.	promoter	2093	2098	.	+	0	ID=annotation1975704;Name=Sigma A-35;Parent=BBa_K143012
BBa_K2145998	.	promoter	2116	2121	.	+	0	ID=annotation1975705;Name=Sigma A -10;Parent=BBa_K143012
BBa_K2145998	.	engineered_region	3385	3428	.	+	0	ID=BBa_K316014;Name=BBa_K316014;Parent=BBa_K316027
BBa_K2145998	.	sequence_feature	3421	3428	.	+	0	ID=BBa_K316013;Name=BBa_K316013;Parent=BBa_K316014
BBa_K2145998	.	sequence_feature	3425	3428	.	+	0	ID=annotation2098166;Name=3' product;Parent=BBa_K316013
BBa_K2145998	.	sequence_feature	3421	3424	.	+	0	ID=annotation2098160;Name=5' product;Parent=BBa_K316013
BBa_K2145998	.	sequence_feature	3385	3412	.	+	0	ID=BBa_K316002;Name=BBa_K316002;Parent=BBa_K316014
BBa_K2145998	.	engineered_region	1	455	.	+	0	ID=BBa_K314110;Name=BBa_K314110
BBa_K2145998	.	sequence_feature	18	324	.	+	0	ID=annotation2115768;Name=f1 origin;Parent=BBa_K314110
>BBa_K2145998
acgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccct
agcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcggggg
ctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtagtg
ggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaac
tggaacaacactcaaccctatctcggtctattcttttgatttataagggattttgccgatttcggcctattggttaaaa
aatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaatttactagatgtttgcaaaac
gattcaaaacctctttactgccgttattcgctggatttttattgctgtttcatttggttctggcaggaccggcggctgc
gagtgctgaaacggcgaacaaatcgaatgagcttacagcaccgtcgatcaaaagcggaaccattcttcatgcatggaat
tggtcgttcaatacgttaaaacacaatatgaaggatattcatgatgcaggatatacagccattcagacatctccgatta
accaagtaaaggaagggaatcaaggagataaaagcatgtcgaactggtactggctgtatcagccgacatcgtatcaaat
tggcaaccgttacttaggtactgaacaagaatttaaagaaatgtgtgcagccgctgaagaatatggcataaaggtcatt
gttgacgcggtcatcaatcataccaccagtgattatgccgcgatttccaatgaggttaagagtattccaaactggacac
atggaaacacacaaattaaaaactggtctgatcgatactagagaattttgtcaaaataattttattgacaacgtcttat
taacgttgatataatttaaattttatttgacaaaaatgggctcgtgttgtacaataaatgttactagagaaaggtggtg
aatactagatgaggaggatatatttgaatacatacgaacaaattaataaagtgaaaaaaatacttcggaaacatttaaa
aaataaccttattggtacttacatgtttggatcaggagttgagagtggactaaaaccaaatagtgatcttgacttttta
gtcgtcgtatctgaaccattgacagatcaaagtaaagaaatacttatacaaaaaattagacctatttcaaaaaaaatag
gagataaaagcaacttacgatatattgaattaacaattattattcagcaagaaatggtaccgtggaatcatcctcccaa
acaagaatttatttatggagaatggttacaagagctttatgaacaaggatacattcctcagaaggaattaaattcagat
ttaaccataatgctttaccaagcaaaacgaaaaaataaaagaatatacggaaattatgacttagaggaattactacctg
atattccattttctgatgtgagaagagccattatggattcgtcagaggaattaatagataattatcaggatgatgaaac
caactctatattaactttatgccgtatgattttaactatggacacgggtaaaatcataccaaaagatattgcgggaaat
gcagtggctgaatcttctccattagaacatagggagagaattttgttagcagttcgtagttatcttggagagaatattg
aatggactaatgaaaatgtaaatttaactataaactatttaaataacagattaaaaaaattataataatactagagcca
ggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcta
ctagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaattttgtcaaaataattttattg
acaacgtcttattaacgttgatataatttaaattttatttgacaaaaatgggctcgtgttgtacaataaatgttactag
agaaaggtggtgaatactagatgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgt
ttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaat
tacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccc
tgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggt
agaacgaagcggcgtcgaagcctgtaaaacggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaac
tatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctg
accagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattggg
tcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatat
ctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgc
aaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccat
taccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatc
ccgccgtcaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagg
gccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaac
cgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtaataa
tactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtg
aacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagatctcctagaata
tatattatgtaaacttactagaggaaatttctactagagatccgtttaggctgggcggtgatagcttctcgttcaggca
gtacgcctcttttcttttccagacctgagggaggcggaaatggtgtgaggttcccggggaaaagccaaataggcgatcg
cgggagtgctttatttgaagatcaggctatcactgcggtcaatagatttcacaatgtgatggctggacagcctgaggaa
ctctcgaacccgaatggaaacaaccagatatttatgaatcagcgcggctcacatggcgttgtgctggcaaatgcaggtt
catcctctgtctctatcaatacggcaacaaaattgcctgatggcaggtatgacaataaagctggagcgggttcatttca
agtgaacgatggtaaactgacaggcacgatcaatgccaggtctgtagctgtgctttatcctgatgatattgcaaaagcg
cctcatgttttccttgagaattacaaaacaggtgtaacacattctttcaatgatcaactgacgattaccttgcgtgcag
atgcgaatacaacaaaagccgtttatcaaatcaataatggaccagagacggcgtttaaggatggagatcaattcacaat
cggaaaaggagatccatttggcaaaacatacaccatcatgttaaaaggaacgaacagtgatggtgtaacgaggaccgag
aaatacagttttgttaaaagagatccagcgtcggccaaaaccatcggctatcaaaatccgaatcattggagccaggtaa
atgcttatatctataaacatgatgggagccgagtaattgaattgaccggatcttggcctggaaaaccaatgactaaaaa
tgcagacggaatttacacgctgacgctgcctgcggacacggatacaaccaacgcaaaagtgatttttaataatggcagc
gcccaagtgcccggtcagaatcagcctggctttgattacgtgctaaatggtttatataatgactcgggcttaagcggtt
ctcttccccattga