LOCUS       BBa_K2148014             258 bp    DNA     linear       23-May-2024
DEFINITION  gRNA for CGG PAM
ACCESSION   BBa_K2148014
VERSION     BBa_K2148014.1  
FEATURES             Location/Qualifiers
     promoter        5..149
                     /label=psaA1 promoter
     misc_feature    152..171
                     /label=target gRNA
     misc_feature    172..254
                     /label=gRNA scaffold
     misc_feature    255..258
                     /label=Phytobrick fusion site
     misc_feature    1..4
                     /label=Phytobrick fusion site
     misc_feature    150..151
                     /label=two extra Guanine for gRNA stability
ORIGIN
        1 ggagtcttaa ttcaacattt ttaagtaaat actgtttaat gttatacttt tacgaataca
       61 catatggtaa aaaataaaac aatatcttta aaataagtaa aaataatttg taaaccaata
      121 aaaaatatat ttatggtata atataacatg gtattaacgc gtttatctta agttttagag
      181 ctagaaatag caagttaaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag
      241 tcggtgcttt ttttgctt
//