BBa_K2148014 1 BBa_K2148014 gRNA for CGG PAM 2016-10-11T11:00:00Z 2016-10-19T01:30:20Z Target sequence designed using Benchling software (https://benchling.com/crispr), scaffold sequence from Addgene (https://www.addgene.org/static/cms/files/hCRISPR_gRNA_Synthesis.pdf) gRNA guiding Cas9 to trnE2-psbH intergenic region of the Chlamydomonas reinhardtii chloroplast genome. The part has a psaA promoter, coded as PROMOTER/5UTR/NTAG/CDS/CTAG Phytobrick. false false _2618_ 33032 33024 9 false 2 Gs added after TSS to stabilise gRNA, following advice of N. Patron (Earlham Institute) false Ciara McCarthy annotation2496581 1 psaA1 promoter range2496581 1 5 149 annotation2522182 1 Phytobrick fusion site range2522182 1 1 4 annotation2497362 1 Phytobrick fusion site range2497362 1 255 258 annotation2496583 1 target gRNA range2496583 1 152 171 annotation2496585 1 gRNA scaffold range2496585 1 172 254 annotation2522183 1 two extra Guanine for gRNA stability range2522183 1 150 151 BBa_K2148014_sequence 1 ggagtcttaattcaacatttttaagtaaatactgtttaatgttatacttttacgaatacacatatggtaaaaaataaaacaatatctttaaaataagtaaaaataatttgtaaaccaataaaaaatatatttatggtataatataacatggtattaacgcgtttatcttaagttttagagctagaaatagcaagttaaaataaggctagtccgttatcaacttgaaaaagtggcaccgagtcggtgctttttttgctt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z