BBa_K2148015
1
BBa_K2148015
gRNA for AGG PAM
2016-10-12T11:00:00Z
2016-10-13T01:23:59Z
Chlamydomonas reinhardtii
This contains an sgRNA which has 20 nucleotide complementary sequences with the Chlamydomonas reinhardtii chloroplast genome upstream of the cut-site, which is at the trnE2-psbH integenic region with psaA promoter. This can be used together with Cas9 to perform site-specific cutting. This part is coded as a Promoter/5UTR/NTAG/CDS/CTAG Phytobrick.
false
false
_2618_
33032
33032
9
false
The target sequence was generated by Alex Mayorov using Benchling software and the scaffold sequence was obtained from Addgene.
false
Chern Juin Ong
annotation2499292
1
Promoter
range2499292
1
5
149
annotation2499296
1
target RNA
range2499296
1
152
171
annotation2499294
1
Additional GG for stability
range2499294
1
150
151
annotation2499291
1
Phytobrick fusion site
range2499291
1
1
4
annotation2499293
1
TSS
range2499293
1
149
149
annotation2499297
1
gRNA scaffold
range2499297
1
172
254
annotation2499298
1
Phytobrick fusion site
range2499298
1
255
258
BBa_K2148015_sequence
1
ggagtcttaattcaacatttttaagtaaatactgtttaatgttatacttttacgaatacacatatggtaaaaaataaaacaatatctttaaaataagtaaaaataatttgtaaaccaataaaaaatatatttatggtataatataacatggaacgcgtttatcttaacggagttttagagctagaaatagcaagttaaaataaggctagtccgttatcaacttgaaaaagtggcaccgagtcggtgctttttttgctt
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z