BBa_K2149000
1
BBa_K2149000
crtE (geranylgeranyl diphosphate synthase)
2016-10-12T11:00:00Z
2016-10-13T06:20:47Z
This part was synthesized by IDT, and it comes from the genomic sequence of Pantoea ananatis. The sequence was assigned to us by Dr. Christoph Albermann, who used it to write the article "Biosynthesis of the Vitamin E Compound δ-Tocotrienol in Recombinant Escherichia coli Cells".
Geranylgeranylpyrophosphate (GGPP) requires the condensation of farnesylpyrophosphate (FPP) and isoprenylpyrophosphate (IPP) by geranylgeranylpyrophosphate synthase (crtE). Although this sequence of crtE is from Pantoea ananatis (one of the crt genes in the biosynthesis of carotenoids in this organism), Geranylgeranylpyrophosphate (GGPP) is also an intermediate in multiple terpenes and terpenoids biosynthesis pathways. In the Synechocystis genome, there is one gene, crtE (slr0739), annotated as geranylgeranyl diphosphate synthase, which is likely to encode the enzyme that performs these steps, and this gene has been used in the pathway for squalene synthesis. In plants it is also the precursor to carotenoids, gibberellins, tocopherols, and chlorophylls.
false
false
_2619_
31116
31116
9
false
The gene was synthesized with thirty nucleotides in its ends to do a Gibson Assembly overlap.
false
Andr?? Tomas Vilela Hermann
annotation2500126
1
crtE
range2500126
1
49
956
annotation2500115
1
BBa_B0034
range2500115
1
37
48
BBa_K2149000_sequence
1
ctaacactgtataacattaagaaggaggtaaaagataaagaggagaaaatgacggtctgcgcaaaaaaacacgttcatctcactcgcgatgctgcggagcagttactggctgatattgatcgacgccttgatcagttattgcccgtggagggagaacgggatgttgtgggtgccgcgatgcgtgaaggtgcgctggcaccgggaaaacgtattcgccccatgttgctgttgctgaccgcccgcgatctgggttgcgctgtcagccatgacggattactggatttggcctgtgcggtggaaatggtccacgcggcttcgctgatccttgacgatatgccctgcatggacgatgcgaagctgcggcgcggacgccctaccattcattctcattacggagagcatgtggcaatactggcggcggttgccttgctgagtaaagcctttggcgtaattgccgatgcagatggcctcacgccgctggcaaaaaatcgggcggtttctgaactgtcaaacgccatcggcatgcaaggattggttcagggtcagttcaaggatctgtctgaaggggataagccgcgcagcgctgaagctattttgatgacgaatcactttaaaaccagcacgctgttttgtgcctccatgcagatggcctcgattgttgcgaatgcctccagcgaagcgcgtgattgcctgcatcgtttttcacttgatcttggtcaggcatttcaactgctggacgatttgaccgatggcatgaccgacaccggtaaggatagcaatcaggacgccggtaaatcgacgctggtcaatctgttaggcccgagggcggttgaagaacgtctgagacaacatcttcagcttgccagtgagcatctctctgcggcctgccaacacgggcacgccactcaacattttattcaggcctggtttgacaaaaaactcgctgccgtcagttatcctcaacgacaggagcacgatcatgc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z