BBa_K2149016 1 BBa_K2149016 luxE (Acyl-protein synthetase) 2016-10-12T11:00:00Z 2016-10-13T08:07:14Z This part was synthesized by IDT, and it comes from the genomic sequence of Photorhabdus luminescens, codon optimized for Escherichia coli. The sequence was taken from the accession number JQ901710, which was used it to write the article "Synthesis of customized petroleum-replica fuel molecules by targeted modification of free fatty acid pools in Escherichia coli". LuxE is an acyl-protein synthetase found in bioluminescent bacteria. LuxE catalyses the formation of an acyl-protein thiolester from a fatty acid and a protein. This is the second step in the bioluminescent fatty acid reduction system, which converts tetradecanoic acid to the aldehyde substrate of the luciferase-catalysed bioluminescence reaction [PMID: 8941351]. This enzyme takes the fatty acid substrate and catalyzes a reaction, forming a acyl-protein thioester product. Together with the LuxC gene, represents the main reaction of the fatty aldehyde synthesis, which can then be used to produce alkanes. false false _2619_ 31116 31116 9 false The gene was synthesized with thirty nucleotides in its ends to do a Gibson Assembly overlap. Its design takes into consideration a RBS and a coding sequence. false Andr?? Tomas Vilela Hermann annotation2500329 1 BBa_B0034 range2500329 1 28 39 annotation2500488 1 luxE range2500488 1 40 1152 BBa_K2149016_sequence 1 ttttcgttccactgagcgtcagaccccaaagaggagaaaatgactagctacgtcgacaaacaggaaatcaccgcgagcagcgagattgacgacctgatcttttccagcgatccgttggtgtggtcctatgatgagcaagaaaagattcgcaagaaactggtcctggatgcgttccgccaccactacaagcactgtcaagagtaccgtcattattgccaagcccataaagtcgacgataacattacggaaattgacgatatcccggttttcccgacctctgttttcaagttcacccgtctgctgacctccaacgagaatgagattgagagctggtttacttcgagcggtaccaatggtctgaaaagccaagtcccgcgtgatcgtctgagcattgaacgtctgctgggcagcgtgagctacggcatgaagtacatcggttcgtggtttgaccatcaaatggagctggttaacttgggtccggatcgctttaatgcccacaacatttggttcaagtacgttatgagcctggttgagctgttgtatccgacgagcttcaccgtgacggaagagcacatcgacttcgtgcagacgctgaacagcctggaacgcattaaacatcagggcaaagacatttgtctgatcggttctccgtatttcatctatctgctgtgccgttacatgaaggacaagaacatcagctttagcggtgacaagagcctgtatatcatcaccggtggcggttggaaaagctacgaaaaagagtccctgaagcgtaatgactttaatcacctgttgttcgatacgttcaatctgagcaacattaaccagatccgtgacatctttaaccaggtcgaactgaatacctgtttctttgaggacgagatgcagcgcaaacacgtcccgccgtgggtatacgcgcgtgcgctggatcctgaaaccttgaaaccggttccagatggcatgcctggtctgatgagctatatggatgctagctctacgagctacccggcatttatcgtgaccgacgatattggtattatcagccgcgagtacggtcaatatccgggcgtgctggttgaaattctgcgtcgtgtgaatacccgcaagcagaaaggctgcgcgttgtctctgacggaggcattcggttcctaaagagtcgatttttgtgatgctcgtcaggg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z