BBa_K2149017
1
BBa_K2149017
Operon Lux C,E and D with an aldehyde decarbonylase
2016-10-17T11:00:00Z
2016-10-18T05:51:36Z
The sequences of operon Lux C, E and D was taken from Photorhabdus luminescens as describe by the work of Dr. Howard and collaborators called "Synthesis of customized petroleum-replica fuel molecules by targeted modification of free fatty acid pools in Escherichia coli".
The gene of the aldehyde decarbonylase was taken from Synechococcus elongatus, a unicellular cyanobacterium and add after the genes of operon Lux.
The operon Lux formed by the genes C, E and D are components of a fatty acid reductase system with reductase, synthetase and transferase activities, respectively. Using fatty acids as a substrate for alkanes biosynthesis, the Lux D gene provides a fatty acid substrate for the fatty acyl synthetase LuxE, that act cleaving activated fatty acids. The fatty acyl reductase subunit (LuxC) then uses the product of the LuxE to produce a fatty aldehyde, an aldehyde with a "fatty" aliphatic carbon chain attached that is typically eight carbon or more in length.
After the production of fatty aldehyde occurs the action of the aldehyde decarbonylase, that catalyzes the conversion of fatty aldehydes to alkanes by the retirade of the aliphatic carbon chain.
false
false
_2619_
31199
31199
9
false
This construction has a RBS before each gene (Lux C, Lux E and Lux D) and a UTR sequence before the gene that encodes an aldehyde decarbonylase.
false
Aline Larissa Gon??alves
annotation2519181
1
B0034
range2519181
1
1482
1495
annotation2519182
1
LuxE
range2519182
1
1496
2607
annotation2519179
1
B0034
range2519179
1
1
12
annotation2519186
1
Aldehyde decarbonylase
range2519186
1
3662
4358
annotation2519185
1
LuxD
range2519185
1
2649
3572
annotation2519184
1
B0034
range2519184
1
2637
2649
annotation2519180
1
LuxC
range2519180
1
13
1455
BBa_K2149017_sequence
1
aaagaggagaaaatgaacaagaaaatcagcttcatcatcaacggtcgcgtagaaatttttccggagtctgatgacctggttcaaagcatcaattttggtgacaatagcgtccacctgccggtgctgaacgatagccaagtgaaaaacattatcgactataatgagaataatgagctgcaactgcacaatatcattaactttctgtataccgtcggtcagcgctggaaaaacgaagaatacagccgtcgtcgtacctatattcgcgatctgaagcgttatatgggctacagcgaggaaatggcgaaactggaagccaattggattagcatgattctgtgctctaaaggtggtttgtacgatctggtgaaaaatgagctgggcagccgtcacattatggacgaatggctgccgcaagacgaaagctacatccgtgccttcccgaaaggcaagagcgttcatctgctgaccggtaatgtcccgctgtcgggcgtgctgtccatcctgcgcgcgattctgaccaagaaccagtgcatcattaagacgagcagcacggatcctttcacggcgaatgcgctggcgctgagcttcatcgacgttgacccacatcacccggtgacccgtagcctgtctgtcgtttattggcagcaccaaggtgacatcagcttggcgaaagagattatgcagcacgccgatgtggtcgttgcctggggtggtgaggatgcaattaactgggcggttaaacacgcaccgccggatatcgacgtcatgaaattcggtccgaaaaagagcttctgcatcattgacaacccggttgacttggttagcgcagcgaccggcgcagcacacgacgtctgtttttacgatcagcaggcatgctttagcacgcagaacatctactacatgggctcccattacgaggagtttaagctggctttgatcgaaaaactgaatctgtatgcacatatcctgcctaacaccaagaaggatttcgacgaaaaggcagcttattccttggtgcaaaaggagtgtctgttcgccggtttgaaagtggaagttgacgttcatcaacgctggatggttattgaatccaatgctggcgttgagctgaaccagccgctgggtcgttgtgtgtacttgcatcacgtggataacatcgagcagattttgccgtatgtgcgtaagaacaaaacccagacgattagcgtgtttccgtgggaggctgcgctgaagtaccgcgatctgctggccctgaaaggcgcggagcgtattgttgaggcgggtatgaataacattttccgtgtgggtggtgcgcacgatggcatgcgtccgctgcaacgcctggtcacttacattagccacgagcgtccgagccattacaccgcgaaggacgtcgcggtcgaaatcgaacagacgcgctttctggaagaggacaagttcctggtgtttgttccataattttcgttccactgagcgtcagaccccaaagaggagaaaatgactagctacgtcgacaaacaggaaatcaccgcgagcagcgagattgacgacctgatcttttccagcgatccgttggtgtggtcctatgatgagcaagaaaagattcgcaagaaactggtcctggatgcgttccgccaccactacaagcactgtcaagagtaccgtcattattgccaagcccataaagtcgacgataacattacggaaattgacgatatcccggttttcccgacctctgttttcaagttcacccgtctgctgacctccaacgagaatgagattgagagctggtttacttcgagcggtaccaatggtctgaaaagccaagtcccgcgtgatcgtctgagcattgaacgtctgctgggcagcgtgagctacggcatgaagtacatcggttcgtggtttgaccatcaaatggagctggttaacttgggtccggatcgctttaatgcccacaacatttggttcaagtacgttatgagcctggttgagctgttgtatccgacgagcttcaccgtgacggaagagcacatcgacttcgtgcagacgctgaacagcctggaacgcattaaacatcagggcaaagacatttgtctgatcggttctccgtatttcatctatctgctgtgccgttacatgaaggacaagaacatcagctttagcggtgacaagagcctgtatatcatcaccggtggcggttggaaaagctacgaaaaagagtccctgaagcgtaatgactttaatcacctgttgttcgatacgttcaatctgagcaacattaaccagatccgtgacatctttaaccaggtcgaactgaatacctgtttctttgaggacgagatgcagcgcaaacacgtcccgccgtgggtatacgcgcgtgcgctggatcctgaaaccttgaaaccggttccagatggcatgcctggtctgatgagctatatggatgctagctctacgagctacccggcatttatcgtgaccgacgatattggtattatcagccgcgagtacggtcaatatccgggcgtgctggttgaaattctgcgtcgtgtgaatacccgcaagcagaaaggctgcgcgttgtctctgacggaggcattcggttcctaaagagtcgatttttgtgatgctcgtcagggaaagaggagaaaatggaaaacgagagcaagtacaaaacgatcgaccacgtaatctgcgtggagggtaacaaaaagattcacgtgtgggagactttgccagaagagaacagcccgaaacgcaaaaacgcaatcattatcgcgagcggtttcgcacgccgcatggatcattttgcgggcctggccgaatacctgagccgtaacggcttccacgttatccgttatgacagcctgcatcacgtcggcctgtcgtctggtaccatcgacgagttcacgatgagcatcggcaagcaaagcctgttggcggttgttgattggctgaccacgcgtaagatcaacaattttggtatgctggcttccagcctgtccgcacgcattgcgtacgcttctctgagcgagattaatgccagctttctgatcaccgccgtgggtttcgtcaatctgcgttatagcctggagcgtgcgctgggtttcgattacttgagcctgccgattaacgagctgccgaataatctggactttgaaggccataagttgggtgcggaggtctttgcgcgtgattgcctggattttggttgggaagatctggcatcgacgattaacaatatgatgtatctggatatcccgtttattgctttcacggcgaataacgacaattgggttaagcaagacgaggttatcaccctgctgtctaacattcgttccaatcgctgtaaaatctatagcttgctgggcagcagccacgacttgagcgaaaatctggtcgtgctgcgcaacttctaccagagcgtgaccaaagcagcgattgcaatggataacgaccacctggacattgacgtggatatcaccgaaccgagcttcgaacatctgaccatcgcgaccgttaacgaacgtcgtatgcgtattgagattgagaatcaggccatttccctgagctaaataccgctagagtgtagattggtgcgtaccccctgggatccaccaaatcccacattctccctctcatccgtgtcaggagaccgcagactcatgccgcagcttgaagccagccttgaactggactttcaaagcgagtcctacaaagacgcttacagccgcatcaacgcgatcgtgattgaaggcgaacaagaggcgttcgacaactacaatcgccttgctgagatgctgcccgaccagcgggatgagcttcacaagctagccaagatggaacagcgccacatgaaaggctttatggcctgtggcaaaaatctctccgtcactcctgacatgggttttgcccagaaatttttcgagcgcttgcacgagaacttcaaagcggcggctgcggaaggcaaggtcgtcacctgcctactgattcaatcgctaatcatcgagtgctttgcgatcgcggcttacaacatctacatcccagtggcggatgcttttgcccgcaaaatcacggagggggtcgtgcgcgacgaatacctgcaccgcaacttcggtgaagagtggctgaaggcgaattttgatgcttccaaagccgaactggaagaagccaatcgtcagaacctgcccttggtttggctaatgctcaacgaagtggccgatgatgctcgcgaactcgggatggagcgtgagtcgctcgtcgaggactttatgattgcctacggtgaagctctggaaaacatcggcttcacaacgcgcgaaatcatgcgtatgtccgcctatggccttgcggccgtttga
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z