BBa_K2149017 1 BBa_K2149017 Operon Lux C,E and D with an aldehyde decarbonylase 2016-10-17T11:00:00Z 2016-10-18T05:51:36Z The sequences of operon Lux C, E and D was taken from Photorhabdus luminescens as describe by the work of Dr. Howard and collaborators called "Synthesis of customized petroleum-replica fuel molecules by targeted modification of free fatty acid pools in Escherichia coli". The gene of the aldehyde decarbonylase was taken from Synechococcus elongatus, a unicellular cyanobacterium and add after the genes of operon Lux. The operon Lux formed by the genes C, E and D are components of a fatty acid reductase system with reductase, synthetase and transferase activities, respectively. Using fatty acids as a substrate for alkanes biosynthesis, the Lux D gene provides a fatty acid substrate for the fatty acyl synthetase LuxE, that act cleaving activated fatty acids. The fatty acyl reductase subunit (LuxC) then uses the product of the LuxE to produce a fatty aldehyde, an aldehyde with a "fatty" aliphatic carbon chain attached that is typically eight carbon or more in length. After the production of fatty aldehyde occurs the action of the aldehyde decarbonylase, that catalyzes the conversion of fatty aldehydes to alkanes by the retirade of the aliphatic carbon chain. false false _2619_ 31199 31199 9 false This construction has a RBS before each gene (Lux C, Lux E and Lux D) and a UTR sequence before the gene that encodes an aldehyde decarbonylase. false Aline Larissa Gon??alves annotation2519181 1 B0034 range2519181 1 1482 1495 annotation2519182 1 LuxE range2519182 1 1496 2607 annotation2519179 1 B0034 range2519179 1 1 12 annotation2519186 1 Aldehyde decarbonylase range2519186 1 3662 4358 annotation2519185 1 LuxD range2519185 1 2649 3572 annotation2519184 1 B0034 range2519184 1 2637 2649 annotation2519180 1 LuxC range2519180 1 13 1455 BBa_K2149017_sequence 1 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igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z