LOCUS       BBa_K215001              865 bp    DNA     linear       25-Nov-2024
DEFINITION  Secretion Signal and Streptavidin Binding Tags
ACCESSION   BBa_K215001
VERSION     BBa_K215001.1  
FEATURES             Location/Qualifiers
     misc_feature    1..48
                     /label=nanotag
     misc_feature    49..57
                     /label=GSA linker
     misc_feature    58..75
                     /label=His tag
     misc_feature    76..84
                     /label=GSA linker
     misc_feature    85..104
                     /label=TEV protease site
     misc_feature    105..114
                     /label=GSA linker
     misc_feature    115..120
                     /label=NheI
     misc_feature    121..129
                     /label=GSA linker
     misc_feature    130..150
                     /label=TEV protease site
     misc_feature    151..159
                     /label=GSA linker
     misc_feature    160..177
                     /label=His tag
     misc_feature    178..186
                     /label=GSA linker
     misc_feature    187..735
                     /label=Secretion tag
     misc_feature    736..865
                     /label=terminator (B0015)
ORIGIN
        1 atggacgttg aagcatggct gggtgcccgt gtaccgctgg ttgaaacggg cagcgcacat
       61 caccaccatc atcatggttc cgccgaaaac ctctactttc agggcggctc tgctgctagc
      121 ggttctgccg agaatctgta cttccaaggt ggctccgctc atcaccacca tcatcacggt
      181 agcgcaggcg gcactgacac ctttgacttc tctggttact ctaacaatca gcgtatcaac
      241 ctgaacgagg gttctttctc tgacgttggt ggtctgaagg gcaacgtttc tatcgcgcat
      301 ggtgttacca tcgaaaacgc gatcggtggt tctggtaacg acatcctgat cggtaatggc
      361 gcggataaca tcctccaggg tggtgcgggt gacgacgttc tgtacggttc tacgggtgcg
      421 gacacgctga cgggtggcgc tggtcgtgac attttcgtat atggtagcgg tcaagactct
      481 accgtttctg catacgactg gatcacggat tttcagacgg gtatcgacaa aatcgacctg
      541 tccgctttcc gtaatgaagg tcagctgtct tttgttcagg accagtttac tggtaaaggc
      601 caggaggtta tgctgcaatg ggacgcggcg aactctacga ccaatctgtg gctgcacgaa
      661 gcgggtcact cttctgttga ctttctcgtt cgtatcgttg gccagaccgc gcagagcgac
      721 attatcgtct aataaccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
      781 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt
      841 cgggtgggcc tttctgcgtt tatat
//