BBa_K2150010
1
BBa_K2150010
This part consists of a gene encoding toxin 134 with a LacI gene, a Ptac promoter included
2016-10-05T11:00:00Z
2016-10-13T10:02:17Z
This gene is acquired by PCR from genome of Bacillus subtilis
This part contain sequence encoding EndoA, a toxin gene in bacteria.
true
false
_2620_
29734
29734
9
false
We used RBS BBa_B0033 to reduce leakage, and there is a RiboJ (an insulator element) directly upstream to the CDS in order to reduce the background noise.
false
Zepeng Mu
component2486562
1
BBa_B0033
annotation2486562
1
BBa_B0033
range2486562
1
1
11
BBa_B0033
1
BBa_B0033
RBS.4 (weaker) -- derivative of BBa_0030
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Weaker RBS based on Ron Weiss thesis. Strengths relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>.
false
true
_41_44_48_46_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-3" in figure 4-14 of thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation7028
1
BBa_B0033
range7028
1
1
11
annotation1713
1
RBS-4\Weaker
range1713
1
1
11
annotation1714
1
RBS
range1714
1
7
10
BBa_K2150010_sequence
1
tcacacaggac
BBa_B0033_sequence
1
tcacacaggac
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z