LOCUS       BBa_K2150015            2152 bp    DNA     linear       22-Nov-2024
DEFINITION  pTet TetX-GFP(fusion protein)
ACCESSION   BBa_K2150015
VERSION     BBa_K2150015.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..1251
                     /label=TetX
     misc_feature    1252..1295
                     /label=protein linker
     CDS             1296..2015
                     /label=GFP
     CDS             81..2015
                     /label=BBa_K2150013
     stem_loop       2035..2078
                     /label=stem_loop
     misc_feature    2024..2103
                     /label=BBa_B0010
     terminator      2024..2103
                     /label=BBa_B0010
     stem_loop       2119..2138
                     /label=T7 TE
     misc_feature    2145..2145
                     /label=stop
     polyA_site      2139..2152
                     /label=polya
     misc_feature    2112..2152
                     /label=BBa_B0012
     terminator      2112..2152
                     /label=BBa_B0012
     terminator      2024..2152
                     /label=BBa_B0015
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgaaaaaaa ccatgcgtat cgacaccgac aaacagatga
      121 acctgctgtc tgacaaaaac gttgcgatca tcggtggtgg tccggttggt ctgaccatgg
      181 cgaaactgct tcagcagaac ggtatcgacg tttctgttta cgaacgtgac aacgaccgtg
      241 aagcgcgtat cttcggtggt accctggacc tgcacaaagg ttctggtcag gaagcgatga
      301 aaaaagcggg tctgcttcag acctactacg acctggcgct gccgatgggt gttaacatcg
      361 cggacaaaaa aggtaacatc ctgtctacca aaaacgttaa accggaaaac cgtttcgaca
      421 acccggaaat caaccgtaac gacctgcgtg cgatcctgct gaactctctg gaaaacgaca
      481 ccgttatctg ggaccgtaaa ctggttatgc tggaaccggg taaaaaaaaa tggaccctga
      541 ccttcgaaaa caaaccgtct gaaaccgcgg acctggttat cctggcgaac ggtggtatgt
      601 ctaaagttcg taaattcgtt accgacaccg aagttgaaga aaccggtacc ttcaacatcc
      661 aggcggacat ccaccagccg gaaatcaact gcccgggttt cttccagctg tgcaacggta
      721 accgtctgat ggcgtctcac cagggtaacc tgctgttcgc gaacccgaac aacaacggtg
      781 cgctgcactt cggtatctct ttcaaaaccc cggacgaatg gaaaaaccag acccaggttg
      841 acttccagaa ccgtaactct gttgttgact tcctgctgaa agagttctct gactgggacg
      901 aacgttacaa agaactgatc cacaccaccc tgtctttcgt tggtctggcg acccgtatct
      961 tcccgctgga aaaaccgtgg aaatctaaac gtccgctgcc gatcaccatg atcggtgacg
     1021 cggcgcacct gatgccgccg ttcgcgggtc agggtgttaa ctctggtctg gttgacgcgc
     1081 tgatcctgtc tgacaacctg gcggacggta aattcaactc tatcgaagaa gcggttaaaa
     1141 actacgaaca gcagatgttc atgtacggta aagaagcgca ggaagaatct acccagaacg
     1201 aaatcgaaat gttcaaaccg gacttcacct tccagcagct gctgaacgtt ggtggaggag
     1261 gttctggagg cggtggaagt ggtggcggag gtagcatgcg taaaggagaa gaacttttca
     1321 ctggagttgt cccaattctt gttgaattag atggtgatgt taatgggcac aaattttctg
     1381 tcagtggaga gggtgaaggt gatgcaacat acggaaaact tacccttaaa tttatttgca
     1441 ctactggaaa actacctgtt ccatggccaa cacttgtcac tactttcggt tatggtgttc
     1501 aatgctttgc gagataccca gatcatatga aacagcatga ctttttcaag agtgccatgc
     1561 ccgaaggtta tgtacaggaa agaactatat ttttcaaaga tgacgggaac tacaagacac
     1621 gtgctgaagt caagtttgaa ggtgataccc ttgttaatag aatcgagtta aaaggtattg
     1681 attttaaaga agatggaaac attcttggac acaaattgga atacaactat aactcacaca
     1741 atgtatacat catggcagac aaacaaaaga atggaatcaa agttaacttc aaaattagac
     1801 acaacattga agatggaagc gttcaactag cagaccatta tcaacaaaat actccaattg
     1861 gcgatggccc tgtcctttta ccagacaacc attacctgtc cacacaatct gccctttcga
     1921 aagatcccaa cgaaaagaga gaccacatgg tccttcttga gtttgtaaca gctgctggga
     1981 ttacacatgg catggatgaa ctatacaaat aataatacta gagccaggca tcaaataaaa
     2041 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
     2101 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta ta
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