LOCUS       BBa_K2150016             903 bp    DNA     linear       23-Dec-2024
DEFINITION  pT7 GFP
ACCESSION   BBa_K2150016
VERSION     BBa_K2150016.1  
FEATURES             Location/Qualifiers
     promoter        1..20
                     /label=BBa_K2150031
     misc_feature    33..36
                     /label=conserved
     RBS             29..40
                     /label=BBa_B0034
     CDS             47..766
                     /label=GFP protein
     CDS             47..766
                     /label=BBa_E0040
     stem_loop       786..829
                     /label=stem_loop
     misc_feature    775..854
                     /label=BBa_B0010
     terminator      775..854
                     /label=BBa_B0010
     stem_loop       870..889
                     /label=T7 TE
     misc_feature    896..896
                     /label=stop
     polyA_site      890..903
                     /label=polya
     misc_feature    863..903
                     /label=BBa_B0012
     terminator      863..903
                     /label=BBa_B0012
     terminator      775..903
                     /label=BBa_B0015
ORIGIN
        1 taatacgact cactataggg tactagagaa agaggagaaa tactagatgc gtaaaggaga
       61 agaacttttc actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca
      121 caaattttct gtcagtggag agggtgaagg tgatgcaaca tacggaaaac ttacccttaa
      181 atttatttgc actactggaa aactacctgt tccatggcca acacttgtca ctactttcgg
      241 ttatggtgtt caatgctttg cgagataccc agatcatatg aaacagcatg actttttcaa
      301 gagtgccatg cccgaaggtt atgtacagga aagaactata tttttcaaag atgacgggaa
      361 ctacaagaca cgtgctgaag tcaagtttga aggtgatacc cttgttaata gaatcgagtt
      421 aaaaggtatt gattttaaag aagatggaaa cattcttgga cacaaattgg aatacaacta
      481 taactcacac aatgtataca tcatggcaga caaacaaaag aatggaatca aagttaactt
      541 caaaattaga cacaacattg aagatggaag cgttcaacta gcagaccatt atcaacaaaa
      601 tactccaatt ggcgatggcc ctgtcctttt accagacaac cattacctgt ccacacaatc
      661 tgccctttcg aaagatccca acgaaaagag agaccacatg gtccttcttg agtttgtaac
      721 agctgctggg attacacatg gcatggatga actatacaaa taataatact agagccaggc
      781 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
      841 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
      901 ata
//