LOCUS       BBa_K2150017            2118 bp    DNA     linear       22-Dec-2024
DEFINITION  pT7 TetX-GFP(fusion protein)
ACCESSION   BBa_K2150017
VERSION     BBa_K2150017.1  
FEATURES             Location/Qualifiers
     promoter        1..20
                     /label=BBa_K2150031
     misc_feature    33..36
                     /label=conserved
     RBS             29..40
                     /label=BBa_B0034
     CDS             47..1217
                     /label=TetX
     misc_feature    1218..1261
                     /label=protein linker
     CDS             1262..1981
                     /label=GFP
     CDS             47..1981
                     /label=BBa_K2150013
     stem_loop       2001..2044
                     /label=stem_loop
     misc_feature    1990..2069
                     /label=BBa_B0010
     terminator      1990..2069
                     /label=BBa_B0010
     stem_loop       2085..2104
                     /label=T7 TE
     misc_feature    2111..2111
                     /label=stop
     polyA_site      2105..2118
                     /label=polya
     misc_feature    2078..2118
                     /label=BBa_B0012
     terminator      2078..2118
                     /label=BBa_B0012
     terminator      1990..2118
                     /label=BBa_B0015
ORIGIN
        1 taatacgact cactataggg tactagagaa agaggagaaa tactagatga aaaaaaccat
       61 gcgtatcgac accgacaaac agatgaacct gctgtctgac aaaaacgttg cgatcatcgg
      121 tggtggtccg gttggtctga ccatggcgaa actgcttcag cagaacggta tcgacgtttc
      181 tgtttacgaa cgtgacaacg accgtgaagc gcgtatcttc ggtggtaccc tggacctgca
      241 caaaggttct ggtcaggaag cgatgaaaaa agcgggtctg cttcagacct actacgacct
      301 ggcgctgccg atgggtgtta acatcgcgga caaaaaaggt aacatcctgt ctaccaaaaa
      361 cgttaaaccg gaaaaccgtt tcgacaaccc ggaaatcaac cgtaacgacc tgcgtgcgat
      421 cctgctgaac tctctggaaa acgacaccgt tatctgggac cgtaaactgg ttatgctgga
      481 accgggtaaa aaaaaatgga ccctgacctt cgaaaacaaa ccgtctgaaa ccgcggacct
      541 ggttatcctg gcgaacggtg gtatgtctaa agttcgtaaa ttcgttaccg acaccgaagt
      601 tgaagaaacc ggtaccttca acatccaggc ggacatccac cagccggaaa tcaactgccc
      661 gggtttcttc cagctgtgca acggtaaccg tctgatggcg tctcaccagg gtaacctgct
      721 gttcgcgaac ccgaacaaca acggtgcgct gcacttcggt atctctttca aaaccccgga
      781 cgaatggaaa aaccagaccc aggttgactt ccagaaccgt aactctgttg ttgacttcct
      841 gctgaaagag ttctctgact gggacgaacg ttacaaagaa ctgatccaca ccaccctgtc
      901 tttcgttggt ctggcgaccc gtatcttccc gctggaaaaa ccgtggaaat ctaaacgtcc
      961 gctgccgatc accatgatcg gtgacgcggc gcacctgatg ccgccgttcg cgggtcaggg
     1021 tgttaactct ggtctggttg acgcgctgat cctgtctgac aacctggcgg acggtaaatt
     1081 caactctatc gaagaagcgg ttaaaaacta cgaacagcag atgttcatgt acggtaaaga
     1141 agcgcaggaa gaatctaccc agaacgaaat cgaaatgttc aaaccggact tcaccttcca
     1201 gcagctgctg aacgttggtg gaggaggttc tggaggcggt ggaagtggtg gcggaggtag
     1261 catgcgtaaa ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg
     1321 tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg caacatacgg
     1381 aaaacttacc cttaaattta tttgcactac tggaaaacta cctgttccat ggccaacact
     1441 tgtcactact ttcggttatg gtgttcaatg ctttgcgaga tacccagatc atatgaaaca
     1501 gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaaagaa ctatattttt
     1561 caaagatgac gggaactaca agacacgtgc tgaagtcaag tttgaaggtg atacccttgt
     1621 taatagaatc gagttaaaag gtattgattt taaagaagat ggaaacattc ttggacacaa
     1681 attggaatac aactataact cacacaatgt atacatcatg gcagacaaac aaaagaatgg
     1741 aatcaaagtt aacttcaaaa ttagacacaa cattgaagat ggaagcgttc aactagcaga
     1801 ccattatcaa caaaatactc caattggcga tggccctgtc cttttaccag acaaccatta
     1861 cctgtccaca caatctgccc tttcgaaaga tcccaacgaa aagagagacc acatggtcct
     1921 tcttgagttt gtaacagctg ctgggattac acatggcatg gatgaactat acaaataata
     1981 atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
     2041 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg
     2101 ggcctttctg cgtttata
//