LOCUS       BBa_K2150019            2180 bp    DNA     linear       07-Dec-2025
DEFINITION  pT7+pTet+RBS+tetX-GFP(fusion protein)+DT
ACCESSION   BBa_K2150019
VERSION     BBa_K2150019.1  
FEATURES             Location/Qualifiers
     promoter        1..20
                     /label=BBa_K2150031
     misc_feature    29..47
                     /label=TetR 1
     misc_feature    29..82
                     /label=BBa_R0040
     promoter        48..53
                     /label=-35
     misc_feature    54..72
                     /label=TetR 2
     promoter        71..76
                     /label=-10
     promoter        29..82
                     /label=BBa_R0040
     misc_feature    95..98
                     /label=conserved
     RBS             91..102
                     /label=BBa_B0034
     CDS             109..1279
                     /label=TetX
     misc_feature    1280..1323
                     /label=protein linker
     CDS             1324..2043
                     /label=GFP
     CDS             109..2043
                     /label=BBa_K2150013
     stem_loop       2063..2106
                     /label=stem_loop
     misc_feature    2052..2131
                     /label=BBa_B0010
     terminator      2052..2131
                     /label=BBa_B0010
     stem_loop       2147..2166
                     /label=T7 TE
     misc_feature    2173..2173
                     /label=stop
     polyA_site      2167..2180
                     /label=polya
     misc_feature    2140..2180
                     /label=BBa_B0012
     terminator      2140..2180
                     /label=BBa_B0012
     terminator      2052..2180
                     /label=BBa_B0015
ORIGIN
        1 taatacgact cactataggg tactagagtc cctatcagtg atagagattg acatccctat
       61 cagtgataga gatactgagc actactagag aaagaggaga aatactagat gaaaaaaacc
      121 atgcgtatcg acaccgacaa acagatgaac ctgctgtctg acaaaaacgt tgcgatcatc
      181 ggtggtggtc cggttggtct gaccatggcg aaactgcttc agcagaacgg tatcgacgtt
      241 tctgtttacg aacgtgacaa cgaccgtgaa gcgcgtatct tcggtggtac cctggacctg
      301 cacaaaggtt ctggtcagga agcgatgaaa aaagcgggtc tgcttcagac ctactacgac
      361 ctggcgctgc cgatgggtgt taacatcgcg gacaaaaaag gtaacatcct gtctaccaaa
      421 aacgttaaac cggaaaaccg tttcgacaac ccggaaatca accgtaacga cctgcgtgcg
      481 atcctgctga actctctgga aaacgacacc gttatctggg accgtaaact ggttatgctg
      541 gaaccgggta aaaaaaaatg gaccctgacc ttcgaaaaca aaccgtctga aaccgcggac
      601 ctggttatcc tggcgaacgg tggtatgtct aaagttcgta aattcgttac cgacaccgaa
      661 gttgaagaaa ccggtacctt caacatccag gcggacatcc accagccgga aatcaactgc
      721 ccgggtttct tccagctgtg caacggtaac cgtctgatgg cgtctcacca gggtaacctg
      781 ctgttcgcga acccgaacaa caacggtgcg ctgcacttcg gtatctcttt caaaaccccg
      841 gacgaatgga aaaaccagac ccaggttgac ttccagaacc gtaactctgt tgttgacttc
      901 ctgctgaaag agttctctga ctgggacgaa cgttacaaag aactgatcca caccaccctg
      961 tctttcgttg gtctggcgac ccgtatcttc ccgctggaaa aaccgtggaa atctaaacgt
     1021 ccgctgccga tcaccatgat cggtgacgcg gcgcacctga tgccgccgtt cgcgggtcag
     1081 ggtgttaact ctggtctggt tgacgcgctg atcctgtctg acaacctggc ggacggtaaa
     1141 ttcaactcta tcgaagaagc ggttaaaaac tacgaacagc agatgttcat gtacggtaaa
     1201 gaagcgcagg aagaatctac ccagaacgaa atcgaaatgt tcaaaccgga cttcaccttc
     1261 cagcagctgc tgaacgttgg tggaggaggt tctggaggcg gtggaagtgg tggcggaggt
     1321 agcatgcgta aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat
     1381 ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga tgcaacatac
     1441 ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc atggccaaca
     1501 cttgtcacta ctttcggtta tggtgttcaa tgctttgcga gatacccaga tcatatgaaa
     1561 cagcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaaag aactatattt
     1621 ttcaaagatg acgggaacta caagacacgt gctgaagtca agtttgaagg tgataccctt
     1681 gttaatagaa tcgagttaaa aggtattgat tttaaagaag atggaaacat tcttggacac
     1741 aaattggaat acaactataa ctcacacaat gtatacatca tggcagacaa acaaaagaat
     1801 ggaatcaaag ttaacttcaa aattagacac aacattgaag atggaagcgt tcaactagca
     1861 gaccattatc aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat
     1921 tacctgtcca cacaatctgc cctttcgaaa gatcccaacg aaaagagaga ccacatggtc
     1981 cttcttgagt ttgtaacagc tgctgggatt acacatggca tggatgaact atacaaataa
     2041 taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     2101 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg
     2161 tgggcctttc tgcgtttata
//