##gff-version 3
##sequence-region BBa_K215002 1 946
BBa_K215002	.	ribosome_entry_site	64	75	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K215002	.	conserved_region	68	71	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K215002	.	tag	82	946	.	+	0	ID=BBa_K215001;Name=BBa_K215001
BBa_K215002	.	sequence_feature	196	201	.	+	0	ID=annotation2027593;Name=NheI;Parent=BBa_K215001
BBa_K215002	.	sequence_feature	130	138	.	+	0	ID=annotation2027588;Name=GSA linker;Parent=BBa_K215001
BBa_K215002	.	stop_codon	817	946	.	+	0	ID=annotation2027600;Name=terminator (B0015);Parent=BBa_K215001
BBa_K215002	.	sequence_feature	166	185	.	+	0	ID=annotation2027591;Name=TEV protease site;Parent=BBa_K215001
BBa_K215002	.	tag	82	129	.	+	0	ID=annotation2027587;Name=nanotag;Parent=BBa_K215001
BBa_K215002	.	sequence_feature	202	210	.	+	0	ID=annotation2027594;Name=GSA linker;Parent=BBa_K215001
BBa_K215002	.	sequence_feature	232	240	.	+	0	ID=annotation2027596;Name=GSA linker;Parent=BBa_K215001
BBa_K215002	.	sequence_feature	211	231	.	+	0	ID=annotation2027595;Name=TEV protease site;Parent=BBa_K215001
BBa_K215002	.	tag	241	258	.	+	0	ID=annotation2027597;Name=His tag;Parent=BBa_K215001
BBa_K215002	.	sequence_feature	186	195	.	+	0	ID=annotation2027592;Name=GSA linker;Parent=BBa_K215001
BBa_K215002	.	sequence_feature	157	165	.	+	0	ID=annotation2027590;Name=GSA linker;Parent=BBa_K215001
BBa_K215002	.	sequence_feature	259	267	.	+	0	ID=annotation2027598;Name=GSA linker;Parent=BBa_K215001
BBa_K215002	.	tag	268	816	.	+	0	ID=annotation2027599;Name=Secretion tag;Parent=BBa_K215001
BBa_K215002	.	tag	139	156	.	+	0	ID=annotation2027589;Name=His tag;Parent=BBa_K215001
BBa_K215002	.	promoter	1	54	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_K215002	.	promoter	20	25	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_K215002	.	promoter	43	48	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_K215002	.	operator	3	19	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_K215002	.	operator	26	42	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_K215002	.	engineered_region	1	54	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
>BBa_K215002
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatact
agatggacgttgaagcatggctgggtgcccgtgtaccgctggttgaaacgggcagcgcacatcaccaccatcatcatgg
ttccgccgaaaacctctactttcagggcggctctgctgctagcggttctgccgagaatctgtacttccaaggtggctcc
gctcatcaccaccatcatcacggtagcgcaggcggcactgacacctttgacttctctggttactctaacaatcagcgta
tcaacctgaacgagggttctttctctgacgttggtggtctgaagggcaacgtttctatcgcgcatggtgttaccatcga
aaacgcgatcggtggttctggtaacgacatcctgatcggtaatggcgcggataacatcctccagggtggtgcgggtgac
gacgttctgtacggttctacgggtgcggacacgctgacgggtggcgctggtcgtgacattttcgtatatggtagcggtc
aagactctaccgtttctgcatacgactggatcacggattttcagacgggtatcgacaaaatcgacctgtccgctttccg
taatgaaggtcagctgtcttttgttcaggaccagtttactggtaaaggccaggaggttatgctgcaatgggacgcggcg
aactctacgaccaatctgtggctgcacgaagcgggtcactcttctgttgactttctcgttcgtatcgttggccagaccg
cgcagagcgacattatcgtctaataaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtttt
atctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatat