LOCUS BBa_K2150020 3646 bp DNA linear 22-Dec-2024 DEFINITION pT7+pTet+RBS+GFP+RBS+T7RNAP+DT ACCESSION BBa_K2150020 VERSION BBa_K2150020.1 FEATURES Location/Qualifiers promoter 1..20 /label=BBa_K2150031 misc_feature 29..47 /label=TetR 1 misc_feature 29..82 /label=BBa_R0040 promoter 48..53 /label=-35 misc_feature 54..72 /label=TetR 2 promoter 71..76 /label=-10 promoter 29..82 /label=BBa_R0040 misc_feature 95..98 /label=conserved RBS 91..102 /label=BBa_B0034 CDS 109..828 /label=GFP protein CDS 109..828 /label=BBa_E0040 misc_feature 841..844 /label=conserved RBS 837..848 /label=BBa_B0034 promoter 3352..3379 /label=putative E. coli promoter CDS 855..3509 /label=BBa_I2032 stem_loop 3529..3572 /label=stem_loop misc_feature 3518..3597 /label=BBa_B0010 terminator 3518..3597 /label=BBa_B0010 stem_loop 3613..3632 /label=T7 TE misc_feature 3639..3639 /label=stop polyA_site 3633..3646 /label=polya misc_feature 3606..3646 /label=BBa_B0012 terminator 3606..3646 /label=BBa_B0012 terminator 3518..3646 /label=BBa_B0015 ORIGIN 1 taatacgact cactataggg tactagagtc cctatcagtg atagagattg acatccctat 61 cagtgataga gatactgagc actactagag aaagaggaga aatactagat gcgtaaagga 121 gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg 181 cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa acttaccctt 241 aaatttattt gcactactgg aaaactacct gttccatggc caacacttgt cactactttc 301 ggttatggtg ttcaatgctt tgcgagatac ccagatcata tgaaacagca tgactttttc 361 aagagtgcca tgcccgaagg ttatgtacag gaaagaacta tatttttcaa agatgacggg 421 aactacaaga cacgtgctga agtcaagttt gaaggtgata cccttgttaa tagaatcgag 481 ttaaaaggta ttgattttaa agaagatgga aacattcttg gacacaaatt ggaatacaac 541 tataactcac acaatgtata catcatggca gacaaacaaa agaatggaat caaagttaac 601 ttcaaaatta gacacaacat tgaagatgga agcgttcaac tagcagacca ttatcaacaa 661 aatactccaa ttggcgatgg ccctgtcctt ttaccagaca accattacct gtccacacaa 721 tctgcccttt cgaaagatcc caacgaaaag agagaccaca tggtccttct tgagtttgta 781 acagctgctg ggattacaca tggcatggat gaactataca aataataata ctagagaaag 841 aggagaaata ctagatgaac acgattaaca tcgctaagaa cgacttctct gacatcgaac 901 tggctgctat cccgttcaac actctggctg accattacgg tgagcgttta gctcgcgaac 961 agttggccct tgagcatgag tcttacgaga tgggtgaagc acgcttccgc aagatgtttg 1021 agcgtcaact taaagctggt gaggttgcgg ataacgctgc cgccaagcct ctcatcacta 1081 ccctactccc taagatgatt gcacgcatca acgactggtt tgaggaagtg aaagctaagc 1141 gcggcaagcg cccgacagcc ttccagttcc tgcaagaaat caagccggaa gccgtagcgt 1201 acatcaccat taagaccact ctggcttgcc taaccagtgc tgacaataca accgttcagg 1261 ctgtagcaag cgcaatcggt cgggccattg aggacgaggc tcgcttcggt cgtatccgtg 1321 accttgaagc taagcacttc aagaaaaacg ttgaggaaca actcaacaag cgcgtagggc 1381 acgtctacaa gaaagcattt atgcaagttg tcgaggctga catgctctct aagggtctac 1441 tcggtggcga ggcgtggtct tcgtggcata aggaagactc tattcatgta ggagtacgct 1501 gcatcgagat gctcattgag tcaaccggaa tggttagctt acaccgccaa aatgctggcg 1561 tagtaggtca agactctgag actatcgaac tcgcacctga atacgctgag gctatcgcaa 1621 cccgtgcagg tgcgctggct ggcatctctc cgatgttcca accttgcgta gttcctccta 1681 agccgtggac tggcattact ggtggtggct attgggctaa cggtcgtcgt cctctggcgc 1741 tggtgcgtac tcacagtaag aaagcactga tgcgctacga agacgtttac atgcctgagg 1801 tgtacaaagc gattaacatt gcgcaaaaca ccgcatggaa aatcaacaag aaagtcctag 1861 cggtcgccaa cgtaatcacc aagtggaagc attgtccggt cgaggacatc cctgcgattg 1921 agcgtgaaga actcccgatg aaaccggaag acatcgacat gaatcctgag gctctcaccg 1981 cgtggaaacg tgctgccgct gctgtgtacc gcaaggacaa ggctcgcaag tctcgccgta 2041 tcagccttga gttcatgctt gagcaagcca ataagtttgc taaccataag gccatctggt 2101 tcccttacaa catggactgg cgcggtcgtg tttacgctgt gtcaatgttc aacccgcaag 2161 gtaacgatat gaccaaagga ctgcttacgc tggcgaaagg taaaccaatc ggtaaggaag 2221 gttactactg gctgaaaatc cacggtgcaa actgtgcggg tgtcgataag gttccgttcc 2281 ctgagcgcat caagttcatt gaggaaaacc acgagaacat catggcttgc gctaagtctc 2341 cactggagaa cacttggtgg gctgagcaag attctccgtt ctgcttcctt gcgttctgct 2401 ttgagtacgc tggggtacag caccacggcc tgagctataa ctgctccctt ccgctggcgt 2461 ttgacgggtc ttgctctggc atccagcact tctccgcgat gctccgagat gaggtaggtg 2521 gtcgcgcggt taacttgctt cctagtgaaa ccgttcagga catctacggg attgttgcta 2581 agaaagtcaa cgagattcta caagcagacg caatcaatgg gaccgataac gaagtagtta 2641 ccgtgaccga tgagaacact ggtgaaatct ctgagaaagt caagctgggc actaaggcac 2701 tggctggtca atggctggct tacggtgtta ctcgcagtgt gactaagcgt tcagtcatga 2761 cgctggctta cgggtccaaa gagttcggct tccgtcaaca agtgctggaa gataccattc 2821 agccagctat tgattccggc aagggtctga tgttcactca gccgaatcag gctgctggat 2881 acatggctaa gctgatttgg gaatctgtga gcgtgacggt ggtagctgcg gttgaagcaa 2941 tgaactggct taagtctgct gctaagctgc tggctgctga ggtcaaagat aagaagactg 3001 gagagattct tcgcaagcgt tgcgctgtgc attgggtaac tcctgatggt ttccctgtgt 3061 ggcaggaata caagaagcct attcagacgc gcttgaacct gatgttcctc ggtcagttcc 3121 gcttacagcc taccattaac accaacaaag atagcgagat tgatgcacac aaacaggagt 3181 ctggtatcgc tcctaacttt gtacacagcc aagacggtag ccaccttcgt aagactgtag 3241 tgtgggcaca cgagaagtac ggaatcgaat cttttgcact gattcacgac tccttcggta 3301 ccattccggc tgacgctgcg aacctgttca aagcagtgcg cgaaactatg gttgacacat 3361 atgagtcttg tgatgtactg gctgatttct acgaccagtt cgctgaccag ttgcacgagt 3421 ctcaattgga caaaatgcca gcacttccgg ctaaaggtaa cttgaacctc cgtgacatct 3481 tagagtcgga cttcgcgttc gcgtaataat actagagcca ggcatcaaat aaaacgaaag 3541 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac 3601 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata //