##gff-version 3
##sequence-region BBa_K2150020 1 3646
BBa_K2150020	.	CDS	109	828	.	+	0	ID=BBa_E0040;Name=BBa_E0040
BBa_K2150020	.	CDS	109	828	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K2150020	.	CDS	855	3509	.	+	0	ID=BBa_I2032;Name=BBa_I2032
BBa_K2150020	.	promoter	3352	3379	.	+	0	ID=annotation1928674;Name=putative E. coli promoter;Parent=BBa_I2032
BBa_K2150020	.	promoter	1	20	.	+	0	ID=BBa_K2150031;Name=BBa_K2150031
BBa_K2150020	.	ribosome_entry_site	837	848	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K2150020	.	conserved_region	841	844	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K2150020	.	promoter	29	82	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K2150020	.	promoter	71	76	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K2150020	.	non_covalent_binding_site	29	47	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K2150020	.	engineered_region	29	82	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K2150020	.	non_covalent_binding_site	54	72	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K2150020	.	promoter	48	53	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K2150020	.	ribosome_entry_site	91	102	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K2150020	.	conserved_region	95	98	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K2150020	.	terminator	3518	3646	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K2150020	.	terminator	3606	3646	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K2150020	.	polyA_site	3633	3646	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K2150020	.	stem_loop	3613	3632	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K2150020	.	stop_codon	3639	3639	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K2150020	.	engineered_region	3606	3646	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K2150020	.	terminator	3518	3597	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K2150020	.	stem_loop	3529	3572	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K2150020	.	engineered_region	3518	3597	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_K2150020
taatacgactcactatagggtactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgag
cactactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttga
attagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacc
cttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaat
gctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaag
aactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaataga
atcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacaca
atgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaag
cgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctg
tccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctg
ggattacacatggcatggatgaactatacaaataataatactagagaaagaggagaaatactagatgaacacgattaac
atcgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctggctgaccattacggtgagcgtt
tagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtca
acttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgc
atcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgcaagaaatcaagc
cggaagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgctgacaatacaaccgttcaggctgt
agcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaag
aaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgaca
tgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaagactctattcatgtaggagtacgctg
catcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgag
actatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcgctggctggcatctctccgatgttcc
aaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaacggtcgtcgtcctctggc
gctggtgcgtactcacagtaagaaagcactgatgcgctacgaagacgtttacatgcctgaggtgtacaaagcgattaac
attgcgcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtcgccaacgtaatcaccaagtggaagcattgtc
cggtcgaggacatccctgcgattgagcgtgaagaactcccgatgaaaccggaagacatcgacatgaatcctgaggctct
caccgcgtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggctcgcaagtctcgccgtatcagccttgagttc
atgcttgagcaagccaataagtttgctaaccataaggccatctggttcccttacaacatggactggcgcggtcgtgttt
acgctgtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggactgcttacgctggcgaaaggtaaaccaatcgg
taaggaaggttactactggctgaaaatccacggtgcaaactgtgcgggtgtcgataaggttccgttccctgagcgcatc
aagttcattgaggaaaaccacgagaacatcatggcttgcgctaagtctccactggagaacacttggtgggctgagcaag
attctccgttctgcttccttgcgttctgctttgagtacgctggggtacagcaccacggcctgagctataactgctccct
tccgctggcgtttgacgggtcttgctctggcatccagcacttctccgcgatgctccgagatgaggtaggtggtcgcgcg
gttaacttgcttcctagtgaaaccgttcaggacatctacgggattgttgctaagaaagtcaacgagattctacaagcag
acgcaatcaatgggaccgataacgaagtagttaccgtgaccgatgagaacactggtgaaatctctgagaaagtcaagct
gggcactaaggcactggctggtcaatggctggcttacggtgttactcgcagtgtgactaagcgttcagtcatgacgctg
gcttacgggtccaaagagttcggcttccgtcaacaagtgctggaagataccattcagccagctattgattccggcaagg
gtctgatgttcactcagccgaatcaggctgctggatacatggctaagctgatttgggaatctgtgagcgtgacggtggt
agctgcggttgaagcaatgaactggcttaagtctgctgctaagctgctggctgctgaggtcaaagataagaagactgga
gagattcttcgcaagcgttgcgctgtgcattgggtaactcctgatggtttccctgtgtggcaggaatacaagaagccta
ttcagacgcgcttgaacctgatgttcctcggtcagttccgcttacagcctaccattaacaccaacaaagatagcgagat
tgatgcacacaaacaggagtctggtatcgctcctaactttgtacacagccaagacggtagccaccttcgtaagactgta
gtgtgggcacacgagaagtacggaatcgaatcttttgcactgattcacgactccttcggtaccattccggctgacgctg
cgaacctgttcaaagcagtgcgcgaaactatggttgacacatatgagtcttgtgatgtactggctgatttctacgacca
gttcgctgaccagttgcacgagtctcaattggacaaaatgccagcacttccggctaaaggtaacttgaacctccgtgac
atcttagagtcggacttcgcgttcgcgtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagac
tgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctt
tctgcgtttata