LOCUS BBa_K2150021 4537 bp DNA linear 22-Dec-2024 DEFINITION pCon+RBS+tetR+DT+pT7+pTet+RBS+GFP+RBS+T7RNAP+DT ACCESSION BBa_K2150021 VERSION BBa_K2150021.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23119 misc_feature 48..51 /label=conserved RBS 44..55 /label=BBa_B0034 CDS 65..681 /label=tetR misc_feature 682..715 /label=SsrA misc_feature 722..746 /label=Help:Barcodes CDS 62..746 /label=BBa_C0040 stem_loop 766..809 /label=stem_loop misc_feature 755..834 /label=BBa_B0010 terminator 755..834 /label=BBa_B0010 stem_loop 850..869 /label=T7 TE misc_feature 876..876 /label=stop polyA_site 870..883 /label=polya misc_feature 843..883 /label=BBa_B0012 terminator 843..883 /label=BBa_B0012 terminator 755..883 /label=BBa_B0015 promoter 892..911 /label=BBa_K2150031 misc_feature 920..938 /label=TetR 1 misc_feature 920..973 /label=BBa_R0040 promoter 939..944 /label=-35 misc_feature 945..963 /label=TetR 2 promoter 962..967 /label=-10 promoter 920..973 /label=BBa_R0040 misc_feature 986..989 /label=conserved RBS 982..993 /label=BBa_B0034 CDS 1000..1719 /label=GFP protein CDS 1000..1719 /label=BBa_E0040 misc_feature 1732..1735 /label=conserved RBS 1728..1739 /label=BBa_B0034 promoter 4243..4270 /label=putative E. coli promoter CDS 1746..4400 /label=BBa_I2032 stem_loop 4420..4463 /label=stem_loop misc_feature 4409..4488 /label=BBa_B0010 terminator 4409..4488 /label=BBa_B0010 stem_loop 4504..4523 /label=T7 TE misc_feature 4530..4530 /label=stop polyA_site 4524..4537 /label=polya misc_feature 4497..4537 /label=BBa_B0012 terminator 4497..4537 /label=BBa_B0012 terminator 4409..4537 /label=BBa_B0015 ORIGIN 1 ttgacagcta gctcagtcct aggtataatg ctagctacta gagaaagagg agaaatacta 61 gatgtccaga ttagataaaa gtaaagtgat taacagcgca ttagagctgc ttaatgaggt 121 cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag ctaggtgtag agcagcctac 181 attgtattgg catgtaaaaa ataagcgggc tttgctcgac gccttagcca ttgagatgtt 241 agataggcac catactcact tttgcccttt agaaggggaa agctggcaag attttttacg 301 taataacgct aaaagtttta gatgtgcttt actaagtcat cgcgatggag caaaagtaca 361 tttaggtaca cggcctacag aaaaacagta tgaaactctc gaaaatcaat tagccttttt 421 atgccaacaa ggtttttcac tagagaatgc attatatgca ctcagcgctg tggggcattt 481 tactttaggt tgcgtattgg aagatcaaga gcatcaagtc gctaaagaag aaagggaaac 541 acctactact gatagtatgc cgccattatt acgacaagct atcgaattat ttgatcacca 601 aggtgcagag ccagccttct tattcggcct tgaattgatc atatgcggat tagaaaaaca 661 acttaaatgt gaaagtgggt ccgctgcaaa cgacgaaaac tacgctttag tagcttaata 721 acactgatag tgctagtgta gatcactact agagccaggc atcaaataaa acgaaaggct 781 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag 841 agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga gtaatacgac 901 tcactatagg gtactagagt ccctatcagt gatagagatt gacatcccta tcagtgatag 961 agatactgag cactactaga gaaagaggag aaatactaga tgcgtaaagg agaagaactt 1021 ttcactggag ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg gcacaaattt 1081 tctgtcagtg gagagggtga aggtgatgca acatacggaa aacttaccct taaatttatt 1141 tgcactactg gaaaactacc tgttccatgg ccaacacttg tcactacttt cggttatggt 1201 gttcaatgct ttgcgagata cccagatcat atgaaacagc atgacttttt caagagtgcc 1261 atgcccgaag gttatgtaca ggaaagaact atatttttca aagatgacgg gaactacaag 1321 acacgtgctg aagtcaagtt tgaaggtgat acccttgtta atagaatcga gttaaaaggt 1381 attgatttta aagaagatgg aaacattctt ggacacaaat tggaatacaa ctataactca 1441 cacaatgtat acatcatggc agacaaacaa aagaatggaa tcaaagttaa cttcaaaatt 1501 agacacaaca ttgaagatgg aagcgttcaa ctagcagacc attatcaaca aaatactcca 1561 attggcgatg gccctgtcct tttaccagac aaccattacc tgtccacaca atctgccctt 1621 tcgaaagatc ccaacgaaaa gagagaccac atggtccttc ttgagtttgt aacagctgct 1681 gggattacac atggcatgga tgaactatac aaataataat actagagaaa gaggagaaat 1741 actagatgaa cacgattaac atcgctaaga acgacttctc tgacatcgaa ctggctgcta 1801 tcccgttcaa cactctggct gaccattacg gtgagcgttt agctcgcgaa cagttggccc 1861 ttgagcatga gtcttacgag atgggtgaag cacgcttccg caagatgttt gagcgtcaac 1921 ttaaagctgg tgaggttgcg gataacgctg ccgccaagcc tctcatcact accctactcc 1981 ctaagatgat tgcacgcatc aacgactggt ttgaggaagt gaaagctaag cgcggcaagc 2041 gcccgacagc cttccagttc ctgcaagaaa tcaagccgga agccgtagcg tacatcacca 2101 ttaagaccac tctggcttgc ctaaccagtg ctgacaatac aaccgttcag gctgtagcaa 2161 gcgcaatcgg tcgggccatt gaggacgagg ctcgcttcgg tcgtatccgt gaccttgaag 2221 ctaagcactt caagaaaaac gttgaggaac aactcaacaa gcgcgtaggg cacgtctaca 2281 agaaagcatt tatgcaagtt gtcgaggctg acatgctctc taagggtcta ctcggtggcg 2341 aggcgtggtc ttcgtggcat aaggaagact ctattcatgt aggagtacgc tgcatcgaga 2401 tgctcattga gtcaaccgga atggttagct tacaccgcca aaatgctggc gtagtaggtc 2461 aagactctga gactatcgaa ctcgcacctg aatacgctga ggctatcgca acccgtgcag 2521 gtgcgctggc tggcatctct ccgatgttcc aaccttgcgt agttcctcct aagccgtgga 2581 ctggcattac tggtggtggc tattgggcta acggtcgtcg tcctctggcg ctggtgcgta 2641 ctcacagtaa gaaagcactg atgcgctacg aagacgttta catgcctgag gtgtacaaag 2701 cgattaacat tgcgcaaaac accgcatgga aaatcaacaa gaaagtccta gcggtcgcca 2761 acgtaatcac caagtggaag cattgtccgg tcgaggacat ccctgcgatt gagcgtgaag 2821 aactcccgat gaaaccggaa gacatcgaca tgaatcctga ggctctcacc gcgtggaaac 2881 gtgctgccgc tgctgtgtac cgcaaggaca aggctcgcaa gtctcgccgt atcagccttg 2941 agttcatgct tgagcaagcc aataagtttg ctaaccataa ggccatctgg ttcccttaca 3001 acatggactg gcgcggtcgt gtttacgctg tgtcaatgtt caacccgcaa ggtaacgata 3061 tgaccaaagg actgcttacg ctggcgaaag gtaaaccaat cggtaaggaa ggttactact 3121 ggctgaaaat ccacggtgca aactgtgcgg gtgtcgataa ggttccgttc cctgagcgca 3181 tcaagttcat tgaggaaaac cacgagaaca tcatggcttg cgctaagtct ccactggaga 3241 acacttggtg ggctgagcaa gattctccgt tctgcttcct tgcgttctgc tttgagtacg 3301 ctggggtaca gcaccacggc ctgagctata actgctccct tccgctggcg tttgacgggt 3361 cttgctctgg catccagcac ttctccgcga tgctccgaga tgaggtaggt ggtcgcgcgg 3421 ttaacttgct tcctagtgaa accgttcagg acatctacgg gattgttgct aagaaagtca 3481 acgagattct acaagcagac gcaatcaatg ggaccgataa cgaagtagtt accgtgaccg 3541 atgagaacac tggtgaaatc tctgagaaag tcaagctggg cactaaggca ctggctggtc 3601 aatggctggc ttacggtgtt actcgcagtg tgactaagcg ttcagtcatg acgctggctt 3661 acgggtccaa agagttcggc ttccgtcaac aagtgctgga agataccatt cagccagcta 3721 ttgattccgg caagggtctg atgttcactc agccgaatca ggctgctgga tacatggcta 3781 agctgatttg ggaatctgtg agcgtgacgg tggtagctgc ggttgaagca atgaactggc 3841 ttaagtctgc tgctaagctg ctggctgctg aggtcaaaga taagaagact ggagagattc 3901 ttcgcaagcg ttgcgctgtg cattgggtaa ctcctgatgg tttccctgtg tggcaggaat 3961 acaagaagcc tattcagacg cgcttgaacc tgatgttcct cggtcagttc cgcttacagc 4021 ctaccattaa caccaacaaa gatagcgaga ttgatgcaca caaacaggag tctggtatcg 4081 ctcctaactt tgtacacagc caagacggta gccaccttcg taagactgta gtgtgggcac 4141 acgagaagta cggaatcgaa tcttttgcac tgattcacga ctccttcggt accattccgg 4201 ctgacgctgc gaacctgttc aaagcagtgc gcgaaactat ggttgacaca tatgagtctt 4261 gtgatgtact ggctgatttc tacgaccagt tcgctgacca gttgcacgag tctcaattgg 4321 acaaaatgcc agcacttccg gctaaaggta acttgaacct ccgtgacatc ttagagtcgg 4381 acttcgcgtt cgcgtaataa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg 4441 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac 4501 actggctcac cttcgggtgg gcctttctgc gtttata //