LOCUS BBa_K2150022 3701 bp DNA linear 18-May-2024 DEFINITION pTet+RBS+T7RNAP+Ter+pT7+RBS+GFP+DT ACCESSION BBa_K2150022 VERSION BBa_K2150022.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 promoter 2578..2605 /label=putative E. coli promoter CDS 81..2735 /label=BBa_I2032 stem_loop 2757..2776 /label=T7 TE misc_feature 2783..2783 /label=stop misc_feature 2780..2780 /label=C misc_feature 2753..2753 /label=C polyA_site 2744..2749 /label='' polyA_site 2777..2790 /label=polya misc_feature 2744..2790 /label=BBa_B0013 terminator 2744..2790 /label=BBa_B0013 promoter 2799..2818 /label=BBa_K2150031 misc_feature 2831..2834 /label=conserved RBS 2827..2838 /label=BBa_B0034 CDS 2845..3564 /label=GFP protein CDS 2845..3564 /label=BBa_E0040 stem_loop 3584..3627 /label=stem_loop misc_feature 3573..3652 /label=BBa_B0010 terminator 3573..3652 /label=BBa_B0010 stem_loop 3668..3687 /label=T7 TE misc_feature 3694..3694 /label=stop polyA_site 3688..3701 /label=polya misc_feature 3661..3701 /label=BBa_B0012 terminator 3661..3701 /label=BBa_B0012 terminator 3573..3701 /label=BBa_B0015 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atgaacacga ttaacatcgc taagaacgac ttctctgaca 121 tcgaactggc tgctatcccg ttcaacactc tggctgacca ttacggtgag cgtttagctc 181 gcgaacagtt ggcccttgag catgagtctt acgagatggg tgaagcacgc ttccgcaaga 241 tgtttgagcg tcaacttaaa gctggtgagg ttgcggataa cgctgccgcc aagcctctca 301 tcactaccct actccctaag atgattgcac gcatcaacga ctggtttgag gaagtgaaag 361 ctaagcgcgg caagcgcccg acagccttcc agttcctgca agaaatcaag ccggaagccg 421 tagcgtacat caccattaag accactctgg cttgcctaac cagtgctgac aatacaaccg 481 ttcaggctgt agcaagcgca atcggtcggg ccattgagga cgaggctcgc ttcggtcgta 541 tccgtgacct tgaagctaag cacttcaaga aaaacgttga ggaacaactc aacaagcgcg 601 tagggcacgt ctacaagaaa gcatttatgc aagttgtcga ggctgacatg ctctctaagg 661 gtctactcgg tggcgaggcg tggtcttcgt ggcataagga agactctatt catgtaggag 721 tacgctgcat cgagatgctc attgagtcaa ccggaatggt tagcttacac cgccaaaatg 781 ctggcgtagt aggtcaagac tctgagacta tcgaactcgc acctgaatac gctgaggcta 841 tcgcaacccg tgcaggtgcg ctggctggca tctctccgat gttccaacct tgcgtagttc 901 ctcctaagcc gtggactggc attactggtg gtggctattg ggctaacggt cgtcgtcctc 961 tggcgctggt gcgtactcac agtaagaaag cactgatgcg ctacgaagac gtttacatgc 1021 ctgaggtgta caaagcgatt aacattgcgc aaaacaccgc atggaaaatc aacaagaaag 1081 tcctagcggt cgccaacgta atcaccaagt ggaagcattg tccggtcgag gacatccctg 1141 cgattgagcg tgaagaactc ccgatgaaac cggaagacat cgacatgaat cctgaggctc 1201 tcaccgcgtg gaaacgtgct gccgctgctg tgtaccgcaa ggacaaggct cgcaagtctc 1261 gccgtatcag ccttgagttc atgcttgagc aagccaataa gtttgctaac cataaggcca 1321 tctggttccc ttacaacatg gactggcgcg gtcgtgttta cgctgtgtca atgttcaacc 1381 cgcaaggtaa cgatatgacc aaaggactgc ttacgctggc gaaaggtaaa ccaatcggta 1441 aggaaggtta ctactggctg aaaatccacg gtgcaaactg tgcgggtgtc gataaggttc 1501 cgttccctga gcgcatcaag ttcattgagg aaaaccacga gaacatcatg gcttgcgcta 1561 agtctccact ggagaacact tggtgggctg agcaagattc tccgttctgc ttccttgcgt 1621 tctgctttga gtacgctggg gtacagcacc acggcctgag ctataactgc tcccttccgc 1681 tggcgtttga cgggtcttgc tctggcatcc agcacttctc cgcgatgctc cgagatgagg 1741 taggtggtcg cgcggttaac ttgcttccta gtgaaaccgt tcaggacatc tacgggattg 1801 ttgctaagaa agtcaacgag attctacaag cagacgcaat caatgggacc gataacgaag 1861 tagttaccgt gaccgatgag aacactggtg aaatctctga gaaagtcaag ctgggcacta 1921 aggcactggc tggtcaatgg ctggcttacg gtgttactcg cagtgtgact aagcgttcag 1981 tcatgacgct ggcttacggg tccaaagagt tcggcttccg tcaacaagtg ctggaagata 2041 ccattcagcc agctattgat tccggcaagg gtctgatgtt cactcagccg aatcaggctg 2101 ctggatacat ggctaagctg atttgggaat ctgtgagcgt gacggtggta gctgcggttg 2161 aagcaatgaa ctggcttaag tctgctgcta agctgctggc tgctgaggtc aaagataaga 2221 agactggaga gattcttcgc aagcgttgcg ctgtgcattg ggtaactcct gatggtttcc 2281 ctgtgtggca ggaatacaag aagcctattc agacgcgctt gaacctgatg ttcctcggtc 2341 agttccgctt acagcctacc attaacacca acaaagatag cgagattgat gcacacaaac 2401 aggagtctgg tatcgctcct aactttgtac acagccaaga cggtagccac cttcgtaaga 2461 ctgtagtgtg ggcacacgag aagtacggaa tcgaatcttt tgcactgatt cacgactcct 2521 tcggtaccat tccggctgac gctgcgaacc tgttcaaagc agtgcgcgaa actatggttg 2581 acacatatga gtcttgtgat gtactggctg atttctacga ccagttcgct gaccagttgc 2641 acgagtctca attggacaaa atgccagcac ttccggctaa aggtaacttg aacctccgtg 2701 acatcttaga gtcggacttc gcgttcgcgt aataatacta gagaaaaaat caaactggct 2761 caccttcggg tgggcctttt tgcgtttata tactagagta atacgactca ctatagggta 2821 ctagagaaag aggagaaata ctagatgcgt aaaggagaag aacttttcac tggagttgtc 2881 ccaattcttg ttgaattaga tggtgatgtt aatgggcaca aattttctgt cagtggagag 2941 ggtgaaggtg atgcaacata cggaaaactt acccttaaat ttatttgcac tactggaaaa 3001 ctacctgttc catggccaac acttgtcact actttcggtt atggtgttca atgctttgcg 3061 agatacccag atcatatgaa acagcatgac tttttcaaga gtgccatgcc cgaaggttat 3121 gtacaggaaa gaactatatt tttcaaagat gacgggaact acaagacacg tgctgaagtc 3181 aagtttgaag gtgataccct tgttaataga atcgagttaa aaggtattga ttttaaagaa 3241 gatggaaaca ttcttggaca caaattggaa tacaactata actcacacaa tgtatacatc 3301 atggcagaca aacaaaagaa tggaatcaaa gttaacttca aaattagaca caacattgaa 3361 gatggaagcg ttcaactagc agaccattat caacaaaata ctccaattgg cgatggccct 3421 gtccttttac cagacaacca ttacctgtcc acacaatctg ccctttcgaa agatcccaac 3481 gaaaagagag accacatggt ccttcttgag tttgtaacag ctgctgggat tacacatggc 3541 atggatgaac tatacaaata ataatactag agccaggcat caaataaaac gaaaggctca 3601 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag 3661 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a //