LOCUS       BBa_K2150025            3043 bp    DNA     linear       01-Jun-2024
DEFINITION  pCon+RBS+tetR+DT+pTet+RBS+tetX-GFP(fusion protein)+DT
ACCESSION   BBa_K2150025
VERSION     BBa_K2150025.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23119
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             65..681
                     /label=tetR
     misc_feature    682..715
                     /label=SsrA
     misc_feature    722..746
                     /label=Help:Barcodes
     CDS             62..746
                     /label=BBa_C0040
     stem_loop       766..809
                     /label=stem_loop
     misc_feature    755..834
                     /label=BBa_B0010
     terminator      755..834
                     /label=BBa_B0010
     stem_loop       850..869
                     /label=T7 TE
     misc_feature    876..876
                     /label=stop
     polyA_site      870..883
                     /label=polya
     misc_feature    843..883
                     /label=BBa_B0012
     terminator      843..883
                     /label=BBa_B0012
     terminator      755..883
                     /label=BBa_B0015
     misc_feature    892..910
                     /label=TetR 1
     misc_feature    892..945
                     /label=BBa_R0040
     promoter        911..916
                     /label=-35
     misc_feature    917..935
                     /label=TetR 2
     promoter        934..939
                     /label=-10
     promoter        892..945
                     /label=BBa_R0040
     misc_feature    958..961
                     /label=conserved
     RBS             954..965
                     /label=BBa_B0034
     CDS             972..2142
                     /label=TetX
     misc_feature    2143..2186
                     /label=protein linker
     CDS             2187..2906
                     /label=GFP
     CDS             972..2906
                     /label=BBa_K2150013
     stem_loop       2926..2969
                     /label=stem_loop
     misc_feature    2915..2994
                     /label=BBa_B0010
     terminator      2915..2994
                     /label=BBa_B0010
     stem_loop       3010..3029
                     /label=T7 TE
     misc_feature    3036..3036
                     /label=stop
     polyA_site      3030..3043
                     /label=polya
     misc_feature    3003..3043
                     /label=BBa_B0012
     terminator      3003..3043
                     /label=BBa_B0012
     terminator      2915..3043
                     /label=BBa_B0015
ORIGIN
        1 ttgacagcta gctcagtcct aggtataatg ctagctacta gagaaagagg agaaatacta
       61 gatgtccaga ttagataaaa gtaaagtgat taacagcgca ttagagctgc ttaatgaggt
      121 cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag ctaggtgtag agcagcctac
      181 attgtattgg catgtaaaaa ataagcgggc tttgctcgac gccttagcca ttgagatgtt
      241 agataggcac catactcact tttgcccttt agaaggggaa agctggcaag attttttacg
      301 taataacgct aaaagtttta gatgtgcttt actaagtcat cgcgatggag caaaagtaca
      361 tttaggtaca cggcctacag aaaaacagta tgaaactctc gaaaatcaat tagccttttt
      421 atgccaacaa ggtttttcac tagagaatgc attatatgca ctcagcgctg tggggcattt
      481 tactttaggt tgcgtattgg aagatcaaga gcatcaagtc gctaaagaag aaagggaaac
      541 acctactact gatagtatgc cgccattatt acgacaagct atcgaattat ttgatcacca
      601 aggtgcagag ccagccttct tattcggcct tgaattgatc atatgcggat tagaaaaaca
      661 acttaaatgt gaaagtgggt ccgctgcaaa cgacgaaaac tacgctttag tagcttaata
      721 acactgatag tgctagtgta gatcactact agagccaggc atcaaataaa acgaaaggct
      781 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag
      841 agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga gtccctatca
      901 gtgatagaga ttgacatccc tatcagtgat agagatactg agcactacta gagaaagagg
      961 agaaatacta gatgaaaaaa accatgcgta tcgacaccga caaacagatg aacctgctgt
     1021 ctgacaaaaa cgttgcgatc atcggtggtg gtccggttgg tctgaccatg gcgaaactgc
     1081 ttcagcagaa cggtatcgac gtttctgttt acgaacgtga caacgaccgt gaagcgcgta
     1141 tcttcggtgg taccctggac ctgcacaaag gttctggtca ggaagcgatg aaaaaagcgg
     1201 gtctgcttca gacctactac gacctggcgc tgccgatggg tgttaacatc gcggacaaaa
     1261 aaggtaacat cctgtctacc aaaaacgtta aaccggaaaa ccgtttcgac aacccggaaa
     1321 tcaaccgtaa cgacctgcgt gcgatcctgc tgaactctct ggaaaacgac accgttatct
     1381 gggaccgtaa actggttatg ctggaaccgg gtaaaaaaaa atggaccctg accttcgaaa
     1441 acaaaccgtc tgaaaccgcg gacctggtta tcctggcgaa cggtggtatg tctaaagttc
     1501 gtaaattcgt taccgacacc gaagttgaag aaaccggtac cttcaacatc caggcggaca
     1561 tccaccagcc ggaaatcaac tgcccgggtt tcttccagct gtgcaacggt aaccgtctga
     1621 tggcgtctca ccagggtaac ctgctgttcg cgaacccgaa caacaacggt gcgctgcact
     1681 tcggtatctc tttcaaaacc ccggacgaat ggaaaaacca gacccaggtt gacttccaga
     1741 accgtaactc tgttgttgac ttcctgctga aagagttctc tgactgggac gaacgttaca
     1801 aagaactgat ccacaccacc ctgtctttcg ttggtctggc gacccgtatc ttcccgctgg
     1861 aaaaaccgtg gaaatctaaa cgtccgctgc cgatcaccat gatcggtgac gcggcgcacc
     1921 tgatgccgcc gttcgcgggt cagggtgtta actctggtct ggttgacgcg ctgatcctgt
     1981 ctgacaacct ggcggacggt aaattcaact ctatcgaaga agcggttaaa aactacgaac
     2041 agcagatgtt catgtacggt aaagaagcgc aggaagaatc tacccagaac gaaatcgaaa
     2101 tgttcaaacc ggacttcacc ttccagcagc tgctgaacgt tggtggagga ggttctggag
     2161 gcggtggaag tggtggcgga ggtagcatgc gtaaaggaga agaacttttc actggagttg
     2221 tcccaattct tgttgaatta gatggtgatg ttaatgggca caaattttct gtcagtggag
     2281 agggtgaagg tgatgcaaca tacggaaaac ttacccttaa atttatttgc actactggaa
     2341 aactacctgt tccatggcca acacttgtca ctactttcgg ttatggtgtt caatgctttg
     2401 cgagataccc agatcatatg aaacagcatg actttttcaa gagtgccatg cccgaaggtt
     2461 atgtacagga aagaactata tttttcaaag atgacgggaa ctacaagaca cgtgctgaag
     2521 tcaagtttga aggtgatacc cttgttaata gaatcgagtt aaaaggtatt gattttaaag
     2581 aagatggaaa cattcttgga cacaaattgg aatacaacta taactcacac aatgtataca
     2641 tcatggcaga caaacaaaag aatggaatca aagttaactt caaaattaga cacaacattg
     2701 aagatggaag cgttcaacta gcagaccatt atcaacaaaa tactccaatt ggcgatggcc
     2761 ctgtcctttt accagacaac cattacctgt ccacacaatc tgccctttcg aaagatccca
     2821 acgaaaagag agaccacatg gtccttcttg agtttgtaac agctgctggg attacacatg
     2881 gcatggatga actatacaaa taataatact agagccaggc atcaaataaa acgaaaggct
     2941 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag
     3001 agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata
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