BBa_K2150025
type: ComponentDefinition
persistent identity: BBa K2150025
display id: BBa_K2150025
version: 1
was generated by: iGEM to SBOL conversion
description: pCon+RBS+tetR+DT+pTet+RBS+tetX-GFP(fusion protein)+DT
created: 2016-10-11T11:00:00Z
modified: 2016-10-17T08:48:10Z
mutable provenance: pCon from BBa_J23119, RBS from BBa_B0034, tetR from BBa_C0040, DT from BBa_B0015,PTet from BBa_R0040 As for fusion protein tetX-GFP, tetX is the same as (xxx), the protein linker is provided by our advisor Li Hua, and the GFP is from BBa_E0040. We combine this three parts using overlap extension polymerase chain reaction (OE-PCR).
owned by: james
owned by: myers
mutable description: In this part, a fusion protein(referred to as tetX-GFP) of tetX(a tetracycline-degrading enzyme) and GFP combined with a 3*GGGGS linker is used to report the expression level of tetX. The first promoter(BBa_J23119) is constitutively on and can express tetR, a protein that can repress the downstream promoter pTet in the absence of tetracycline(Tc). As a result, no fusion protein tetX-GFP is expressed and no fluorescence can be detected. However, in the presence of Tc, tetR is repressed and repression over pTet is removed. This leads to the expression of tetX-GFP, degrading tetracycline and emitting fluorescence at the same time. This part can report activity of tetX in the presence of Tc by emission of green fluorescence.
discontinued: true
dominant: false
experience: experience/None
group u list: _2620_
m user id: 29891
owner id: 31283
owning group id: 9
status: status/Deleted
bookmark: false
mutable notes: Linker for fusion protein is carefully designed to avoid repeated sequence which may cause problems in assembly.
star: false
creator: Jianyi Huang
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_R0040
component: BBa_B0015
component: BBa_K2150013
component: BBa_B0034
component: BBa_J23119
component: BBa_C0040
sequence annotation: BBa_J23119
sequence annotation: BBa_B0034
sequence annotation: BBa_C0040
sequence annotation: BBa_B0015
sequence annotation: BBa_R0040
sequence annotation: BBa_K2150013
sequence: https://synbiohub.org/public/igem/BBa_K2150025_sequence/1

BBa_R0040
type: Component
persistent identity: component2495938
display id: component2495938
version: 1
top level: BBa_K2150025
definition: p(tetR)
access: public

BBa_B0015
type: Component
persistent identity: component2495937
display id: component2495937
version: 1
top level: BBa_K2150025
definition: BBa_B0015
access: public

BBa_K2150013
type: Component
persistent identity: component2495948
display id: component2495948
version: 1
top level: BBa_K2150025
definition: tetX-GFP
access: public

BBa_B0034
type: Component
persistent identity: component2495944
display id: component2495944
version: 1
top level: BBa_K2150025
definition: BBa_B0034
access: public

BBa_B0015
type: Component
persistent identity: component2495955
display id: component2495955
version: 1
top level: BBa_K2150025
definition: BBa_B0015
access: public

BBa_J23119
type: Component
persistent identity: component2495924
display id: component2495924
version: 1
top level: BBa_K2150025
definition: BBa_J23119
access: public

BBa_C0040
type: Component
persistent identity: component2495930
display id: component2495930
version: 1
top level: BBa_K2150025
definition: tetR
access: public

BBa_B0034
type: Component
persistent identity: component2495926
display id: component2495926
version: 1
top level: BBa_K2150025
definition: BBa_B0034
access: public

BBa_J23119
type: SequenceAnnotation
persistent identity: annotation2495924
display id: annotation2495924
version: 1
top level: BBa_K2150025
location: https://synbiohub.org/public/igem/BBa_K2150025/annotation2495924/range2495924/1
component: BBa_J23119

https://synbiohub.org/public/igem/BBa_K2150025/annotation2495924/range2495924/1
type: Range
persistent identity: range2495924
display id: range2495924
version: 1
top level: BBa_K2150025
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2495926
display id: annotation2495926
version: 1
top level: BBa_K2150025
location: https://synbiohub.org/public/igem/BBa_K2150025/annotation2495926/range2495926/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K2150025/annotation2495926/range2495926/1
type: Range
persistent identity: range2495926
display id: range2495926
version: 1
top level: BBa_K2150025
direction: direction/reverse
start: 44
end: 55
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation2495930
display id: annotation2495930
version: 1
top level: BBa_K2150025
location: https://synbiohub.org/public/igem/BBa_K2150025/annotation2495930/range2495930/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_K2150025/annotation2495930/range2495930/1
type: Range
persistent identity: range2495930
display id: range2495930
version: 1
top level: BBa_K2150025
direction: direction/reverse
start: 62
end: 746
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2495937
display id: annotation2495937
version: 1
top level: BBa_K2150025
location: https://synbiohub.org/public/igem/BBa_K2150025/annotation2495937/range2495937/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K2150025/annotation2495937/range2495937/1
type: Range
persistent identity: range2495937
display id: range2495937
version: 1
top level: BBa_K2150025
direction: direction/reverse
start: 755
end: 883
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2495938
display id: annotation2495938
version: 1
top level: BBa_K2150025
location: https://synbiohub.org/public/igem/BBa_K2150025/annotation2495938/range2495938/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K2150025/annotation2495938/range2495938/1
type: Range
persistent identity: range2495938
display id: range2495938
version: 1
top level: BBa_K2150025
direction: direction/forward
start: 892
end: 945
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2495944
display id: annotation2495944
version: 1
top level: BBa_K2150025
location: https://synbiohub.org/public/igem/BBa_K2150025/annotation2495944/range2495944/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K2150025/annotation2495944/range2495944/1
type: Range
persistent identity: range2495944
display id: range2495944
version: 1
top level: BBa_K2150025
direction: direction/reverse
start: 954
end: 965
orientation: inline

BBa_K2150013
type: SequenceAnnotation
persistent identity: annotation2495948
display id: annotation2495948
version: 1
top level: BBa_K2150025
location: https://synbiohub.org/public/igem/BBa_K2150025/annotation2495948/range2495948/1
component: BBa_K2150013

https://synbiohub.org/public/igem/BBa_K2150025/annotation2495948/range2495948/1
type: Range
persistent identity: range2495948
display id: range2495948
version: 1
top level: BBa_K2150025
direction: direction/reverse
start: 972
end: 2906
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2495955
display id: annotation2495955
version: 1
top level: BBa_K2150025
location: https://synbiohub.org/public/igem/BBa_K2150025/annotation2495955/range2495955/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K2150025/annotation2495955/range2495955/1
type: Range
persistent identity: range2495955
display id: range2495955
version: 1
top level: BBa_K2150025
direction: direction/reverse
start: 2915
end: 3043
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

tetR
type: ComponentDefinition
persistent identity: BBa C0040
display id: BBa_C0040
version: 1
was generated by: iGEM to SBOL conversion
description: tetracycline repressor from transposon Tn10 (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the TetR protein without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission. TetR binds to the pTet regulator (BBa_R0040). aTc (anhydrotetracycline) binds to TetR and inhibits its operation.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 References (unparsed) here: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 BBa_C0040 TetR Protein is based on the TetR sequence from Elowitz's repressilator. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman.
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: tetR
sequence annotation: SsrA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0040_sequence/1

tetR
type: SequenceAnnotation
persistent identity: annotation23329
display id: annotation23329
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation23329/range23329/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0040/annotation23329/range23329/1
type: Range
persistent identity: range23329
display id: range23329
version: 1
top level: tetR
direction: direction/forward
start: 4
end: 620
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation23330
display id: annotation23330
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation23330/range23330/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0040/annotation23330/range23330/1
type: Range
persistent identity: range23330
display id: range23330
version: 1
top level: tetR
direction: direction/forward
start: 621
end: 654
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213989
display id: annotation2213989
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation2213989/range2213989/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0040/annotation2213989/range2213989/1
type: Range
persistent identity: range2213989
display id: range2213989
version: 1
top level: tetR
direction: direction/forward
start: 661
end: 685
orientation: inline

BBa_J23119
type: ComponentDefinition
persistent identity: BBa J23119
display id: BBa_J23119
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-23T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Overlap extension of synthetic oligonucleotides
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Later
discontinued: false
dominant: true
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_J23119_sequence/1

tetX-GFP
type: ComponentDefinition
persistent identity: BBa K2150013
display id: BBa_K2150013
version: 1
was generated by: iGEM to SBOL conversion
description: A fusion protein combining tetX(BBa_K2150101) with GFP(BBa_E0040)
created: 2016-10-08T11:00:00Z
modified: 2016-10-19T01:06:12Z
mutable provenance: The source of tetX is the same as (xxx), the protein linker is provided by our advisor Li Hua, and the GFP is from BBa_E0040. We combine this three parts using overlap extension polymerase chain reaction (OE-PCR).
owned by: james
owned by: myers
mutable description: This protein(noted as tetX-GFP) combines a tetracycline-degrading enzyme (tetX monooxygenase, ) with a green fluorescent protein (GFPmt3, BBa_E0040). TetX-GFP has activities of both the tetX and the GFP. As a degradation enzyme, it can degrade tetracycline(tc) and its analogs as we describe (here), and also give the tetracycline resistance to E.coli. As a green fluorescent protein, it inherits the normal function of BBa_E0040. However, the expression quantity of this protein may be smaller than BBa_E0040 under the same promoter or it may take longer time for tetX-GFP to mature, for we notice that when it is expressed under the same promoter as BBa_E0040, the fluorescent intensity(FI) of tetX-GFP is weaker than BBa_E0040 at the same growth stage of E.coli, and it takes longer for E.coli expressing tetX-GFP to reach the same FI as E.coli expressing BBa_E0040. We use tetX-GFP as a monitor of the quantity of tetX expressed in E.coli. TetX-GFP can also act as a reporter of whether our system is working (meaning whether our machine is degrading tetracycline). When tetX-GFP is expressed under a tetracycline-regulated promoter such as BBa_R0040, it will become a visible sensor of tetracycline in the presence of tetR, with no need for additional tetracycline-resistance genes.
discontinued: false
dominant: false
experience: experience/Works
group u list: _2620_
m user id: 29891
owner id: 29891
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: The protein linker is 3*GGGGS which provides flexibility to the fusion protein. When choosing the codon for this linker, we need to avoid repeated sequence which may cause problems when assemble these parts.
star: false
creator: Yang Ye
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: TetX
sequence annotation: protein linker
sequence annotation: GFP
sequence: https://synbiohub.org/public/igem/BBa_K2150013_sequence/1

TetX
type: SequenceAnnotation
persistent identity: annotation2489537
display id: annotation2489537
version: 1
top level: tetX-GFP
location: https://synbiohub.org/public/igem/BBa_K2150013/annotation2489537/range2489537/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_K2150013/annotation2489537/range2489537/1
type: Range
persistent identity: range2489537
display id: range2489537
version: 1
top level: tetX-GFP
direction: direction/forward
start: 1
end: 1171
orientation: inline

protein linker
type: SequenceAnnotation
persistent identity: annotation2489538
display id: annotation2489538
version: 1
top level: tetX-GFP
location: https://synbiohub.org/public/igem/BBa_K2150013/annotation2489538/range2489538/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_K2150013/annotation2489538/range2489538/1
type: Range
persistent identity: range2489538
display id: range2489538
version: 1
top level: tetX-GFP
direction: direction/forward
start: 1172
end: 1215
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation2489539
display id: annotation2489539
version: 1
top level: tetX-GFP
location: https://synbiohub.org/public/igem/BBa_K2150013/annotation2489539/range2489539/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K2150013/annotation2489539/range2489539/1
type: Range
persistent identity: range2489539
display id: range2489539
version: 1
top level: tetX-GFP
direction: direction/forward
start: 1216
end: 1935
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_K2150025_sequence/1
type: Sequence
persistent identity: BBa K2150025 sequence
display id: BBa_K2150025_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctaggtataatgctagctactagagaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgaaaaaaaccatgcgtatcgacaccgacaaacagatgaacctgctgtctgacaaaaacgttgcgatcatcggtggtggtccggttggtctgaccatggcgaaactgcttcagcagaacggtatcgacgtttctgtttacgaacgtgacaacgaccgtgaagcgcgtatcttcggtggtaccctggacctgcacaaaggttctggtcaggaagcgatgaaaaaagcgggtctgcttcagacctactacgacctggcgctgccgatgggtgttaacatcgcggacaaaaaaggtaacatcctgtctaccaaaaacgttaaaccggaaaaccgtttcgacaacccggaaatcaaccgtaacgacctgcgtgcgatcctgctgaactctctggaaaacgacaccgttatctgggaccgtaaactggttatgctggaaccgggtaaaaaaaaatggaccctgaccttcgaaaacaaaccgtctgaaaccgcggacctggttatcctggcgaacggtggtatgtctaaagttcgtaaattcgttaccgacaccgaagttgaagaaaccggtaccttcaacatccaggcggacatccaccagccggaaatcaactgcccgggtttcttccagctgtgcaacggtaaccgtctgatggcgtctcaccagggtaacctgctgttcgcgaacccgaacaacaacggtgcgctgcacttcggtatctctttcaaaaccccggacgaatggaaaaaccagacccaggttgacttccagaaccgtaactctgttgttgacttcctgctgaaagagttctctgactgggacgaacgttacaaagaactgatccacaccaccctgtctttcgttggtctggcgacccgtatcttcccgctggaaaaaccgtggaaatctaaacgtccgctgccgatcaccatgatcggtgacgcggcgcacctgatgccgccgttcgcgggtcagggtgttaactctggtctggttgacgcgctgatcctgtctgacaacctggcggacggt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0040_sequence/1
type: Sequence
persistent identity: BBa C0040 sequence
display id: BBa_C0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23119_sequence/1
type: Sequence
persistent identity: BBa J23119 sequence
display id: BBa_J23119_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctaggtataatgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2150013_sequence/1
type: Sequence
persistent identity: BBa K2150013 sequence
display id: BBa_K2150013_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaaaaaccatgcgtatcgacaccgacaaacagatgaacctgctgtctgacaaaaacgttgcgatcatcggtggtggtccggttggtctgaccatggcgaaactgcttcagcagaacggtatcgacgtttctgtttacgaacgtgacaacgaccgtgaagcgcgtatcttcggtggtaccctggacctgcacaaaggttctggtcaggaagcgatgaaaaaagcgggtctgcttcagacctactacgacctggcgctgccgatgggtgttaacatcgcggacaaaaaaggtaacatcctgtctaccaaaaacgttaaaccggaaaaccgtttcgacaacccggaaatcaaccgtaacgacctgcgtgcgatcctgctgaactctctggaaaacgacaccgttatctgggaccgtaaactggttatgctggaaccgggtaaaaaaaaatggaccctgaccttcgaaaacaaaccgtctgaaaccgcggacctggttatcctggcgaacggtggtatgtctaaagttcgtaaattcgttaccgacaccgaagttgaagaaaccggtaccttcaacatccaggcggacatccaccagccggaaatcaactgcccgggtttcttccagctgtgcaacggtaaccgtctgatggcgtctcaccagggtaacctgctgttcgcgaacccgaacaacaacggtgcgctgcacttcggtatctctttcaaaaccccggacgaatggaaaaaccagacccaggttgacttccagaaccgtaactctgttgttgacttcctgctgaaagagttctctgactgggacgaacgttacaaagaactgatccacaccaccctgtctttcgttggtctggcgacccgtatcttcccgctggaaaaaccgtggaaatctaaacgtccgctgccgatcaccatgatcggtgacgcggcgcacctgatgccgccgttcgcgggtcagggtgttaactctggtctggttgacgcgctgatcctgtctgacaacctggcggacggtaaattcaactctatcgaagaagcggttaaaaactacgaacagcagatgttcatgtacggtaaagaagcgcaggaagaatctacccagaacgaaatcgaaatgttcaaaccggacttcaccttccagcagctgctgaacgttggtggaggaggttctggaggcggtggaagtggtggcggaggtagcatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z